Equine herpes virus 1 (EHV-1) in liver, spleen, and lung as demonstrated by immunohistology and electron microscopy.
Abstract: Ten aborted foals, diagnosed as infected with Equine Herpes Virus 1 (EHV-1) on histopathological criteria, were examined for the presence of EHV-1 using immunohistology as the investigative instrument. The primary reagent was an antiserum specific for viral envelope glycoproteins. Immunohistology localised EHV-1 to areas of liver necrosis and to the cytoplasm of infected Kupffer cells and hepatocytes. Cytoplasmic immunolabelling was also prominent in reticular cells of the red pulp of the spleen and in intact and degenerated bronchiolar epithelium. Cytoplasmic immunolabelling was seen in morphologically unchanged cells and in cells containing intranuclear inclusion bodies. Three aborted foetuses with no histological signs of EHV-1 infection were negative when immunostained for EHV-1. Detection by electron microscopy of EHV-1 virions confirmed the EHV-1 specificity of the immunolabelling procedure.
Publication Date: 1989-01-01 PubMed ID: 2556904PubMed Central: PMC8142178DOI: 10.1186/BF03548050Google Scholar: Lookup
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- Journal Article
Summary
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This research article investigates the presence and localization of Equine Herpes Virus 1 (EHV-1) in the liver, spleen, and lungs of aborted foals using immunohistology and electron microscopy methods.
Introduction to the Study
- The study begins with the examination of ten aborted foals previously diagnosed with EHV-1 based on histopathological criteria.
- Immunohistology, a method that uses antibodies to visualize specific antigens in tissue sections, was the primary investigative tool used in the study.
Methodology & Tools Used
- The primary reagent applied was an antiserum that is specifically used for the detection of viral envelope glycoproteins.
- Electron microscopy was used to confirm the presence of the EHV-1 virions, thus validating the specificity of the immunolabelling process.
Findings
- The immunohistology method localized the presence of EHV-1 to areas of liver necrosis, the cytoplasm of infected hepatocytes, and in Kupffer cells (which are a type of macrophage found in the liver).
- Notably, the study also found that cytoplasmic immunolabelling was prominent in the reticular cells of the spleen’s red pulp and in the bronchiolar epithelium, both in its intact and degenerated state.
- The labelling technique identified EHV-1 in morphologically unchanged cells and also in cells containing intranuclear inclusion bodies, which are a common feature of virus-infected cells.
Control Group
- Three aborted fetuses with no histological signs of EHV-1 infection served as the control group in this study.
- These foals came out negative when immunostained for EHV-1, providing further validation to the method’s specificity.
Conclusion
- The use of immunohistology and electron microscopy in this study effectively demonstrated the presence and localization of EHV-1 in the liver, spleen, and lungs of infected foals.
- Distinct immunolabelling was observed in particular regions and types of cells, shedding light on the virus’s behavior in the host’s body.
Cite This Article
APA
Jönsson L, Beck-Friis J, Renström LH, Nikkilä T, Thebo P, Sundquist B.
(1989).
Equine herpes virus 1 (EHV-1) in liver, spleen, and lung as demonstrated by immunohistology and electron microscopy.
Acta Vet Scand, 30(2), 141-146.
https://doi.org/10.1186/BF03548050 Publication
Researcher Affiliations
MeSH Terms
- Abortion, Veterinary / microbiology
- Animals
- Female
- Herpesviridae / isolation & purification
- Herpesviridae Infections / microbiology
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / microbiology
- Horses
- Liver / microbiology
- Lung / microbiology
- Pregnancy
- Spleen / microbiology
References
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Citations
This article has been cited 1 times.- Perez-Ecija A, Mendoza FJ, Estepa JC, Bautista MJ, Pérez J. Equid herpesvirus 1 and rhodococcus equi coinfection in a foal with bronchointerstitial pneumonia. J Vet Med Sci 2016 Oct 1;78(9):1511-1513.
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