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Home / Equine Research Bank / J Virol / Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Targ...
Journal of virology2019; 93(7); e02098-18; doi: 10.1128/JVI.02098-18

Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs.

Abstract: Equine herpesvirus 1 (EHV1) replicates in the respiratory epithelium and disseminates through the body via a cell-associated viremia in leukocytes, despite the presence of neutralizing antibodies. "Hijacked" leukocytes, previously identified as monocytic cells and T lymphocytes, transmit EHV1 to endothelial cells of the endometrium or central nervous system, causing reproductive (abortigenic variants) or neurological (neurological variants) disorders. In the present study, we questioned the potential route of EHV1 infection of T lymphocytes and how EHV1 misuses T lymphocytes as a vehicle to reach the endothelium of the target organs in the absence or presence of immune surveillance. Viral replication was evaluated in activated and quiescent primary T lymphocytes, and the results demonstrated increased infection of activated versus quiescent, CD4 versus CD8, and blood- versus lymph node-derived T cells. Moreover, primarily infected respiratory epithelial cells and circulating monocytic cells efficiently transferred virions to T lymphocytes in the presence of neutralizing antibodies. Albeit T-lymphocytes express all classes of viral proteins early in infection, the expression of viral glycoproteins on their cell surface was restricted. In addition, the release of viral progeny was hampered, resulting in the accumulation of viral nucleocapsids in the T cell nucleus. During contact of infected T lymphocytes with endothelial cells, a late viral protein(s) orchestrates T cell polarization and synapse formation, followed by anterograde dynein-mediated transport and transfer of viral progeny to the engaged cell. This represents a sophisticated but efficient immune evasion strategy to allow transfer of progeny virus from T lymphocytes to adjacent target cells. These results demonstrate that T lymphocytes are susceptible to EHV1 infection and that cell-cell contact transmits infectious virus to and from T lymphocytes. Equine herpesvirus 1 (EHV1) is an ancestral alphaherpesvirus that is related to herpes simplex virus 1 and causes respiratory, reproductive, and neurological disorders in Equidae. EHV1 is indisputably a master at exploiting leukocytes to reach its target organs, accordingly evading the host immunity. However, the role of T lymphocytes in cell-associated viremia remains poorly understood. Here we show that activated T lymphocytes efficiently become infected and support viral replication despite the presence of protective immunity. We demonstrate a restricted expression of viral proteins on the surfaces of infected T cells, which prevents immune recognition. In addition, we indicate a hampered release of progeny, which results in the accumulation of nucleocapsids in the T cell nucleus. Upon engagement with the target endothelium, late viral proteins orchestrate viral synapse formation and viral transfer to the contact cell. Our findings have significant implications for the understanding of EHV1 pathogenesis, which is essential for developing innovative therapies to prevent the devastating clinical symptoms of infection.
Copyright © 2019 Poelaert et al.
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Publication Date: 2019-03-21 PubMed ID: 30651370PubMed Central: PMC6430527DOI: 10.1128/JVI.02098-18Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on how Equine herpesvirus 1 (EHV1), a disease-causing agent in horses, leverages T lymphocytes (a type of white blood cell) to reach its target organs, causing reproductive or neurological disorders.

Methodology and Key Findings

The researchers examined how the EHV1 infection progresses in T lymphocytes under various conditions:

  • Viral replication was studied in different states of primary T lymphocytes – both activated (responsive) and quiescent (inactive).
  • It was observed that the virus infected a higher number of activated, CD4, and blood-derived T cells compared to quiescent, CD8, and lymph-node derived T cells.
  • Notably, the EHV1 virus was discovered to transfer efficiently from primarily infected respiratory epithelial cells and circulating monocytic cells (another type of white blood cell) to T lymphocytes, even in the presence of neutralizing antibodies.
  • In infected T lymphocytes, while all classes of viral proteins were expressed early, the surface expression of viral glycoproteins was limited.
  • The release of new viruses from the T lymphocytes was found to be limited, causing an accumulation of viral elements in the T cell nucleus.

Interaction of Infected T Lymphocytes with Endothelial Cells

When infected T lymphocytes contact endothelial cells (that line the interior of blood vessels), some interesting processes were observed:

  • A late viral protein helps in organizing cell polarization and synapse formation – a connection between the T lymphocyte and the endothelial cell.
  • This connection enables the forward transport (mediated by a motor protein called dynein) and transfer of new viruses to the endothelial cell.

Conclusion and Implications

The study demonstrated that T lymphocytes are susceptible to EHV1 infection and can transfer the virus to other cells through direct contact. It highlighted a sophisticated immune evasion tactic used by the virus, where restricted expression of viral proteins on infected T cells prevents immune recognition. The study’s findings have significant implications for understanding EHV1’s pathogenesis and potentially developing innovative therapies to prevent the severe clinical symptoms of this infection.

Cite This Article

APA
Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Couck L, Van den Broeck W, Azab W, Nauwynck HJ. (2019). Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs. J Virol, 93(7), e02098-18. https://doi.org/10.1128/JVI.02098-18

Publication

Journal of virology
ISSN: 1098-5514
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 93
Issue: 7
PII: e02098-18

Researcher Affiliations

Poelaert, Katrien C K
  • Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Van Cleemput, Jolien
  • Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Laval, Kathlyn
  • Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
Favoreel, Herman W
  • Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Couck, Liesbeth
  • Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Van den Broeck, Wim
  • Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Azab, Walid
  • Institut fur Virologie, Zentrum fur Infektionsmedezin, Freie Universitat Berlin, Berlin, Germany.
Nauwynck, Hans J
  • Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium hans.nauwynck@ugent.be.

MeSH Terms

  • Animals
  • Cells, Cultured
  • Endothelial Cells / immunology
  • Endothelial Cells / virology
  • Epithelial Cells / immunology
  • Epithelial Cells / virology
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid / immunology
  • Horse Diseases / immunology
  • Horse Diseases / virology
  • Horses / immunology
  • Horses / virology
  • Immune Evasion / immunology
  • Monocytes / immunology
  • Monocytes / virology
  • Respiratory Mucosa / immunology
  • Respiratory Mucosa / virology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / virology
  • Viral Proteins / immunology
  • Viremia / immunology
  • Viremia / virology
  • Virus Replication / immunology

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