Equine herpesvirus-1 infection induces IFN-gamma production by equine T lymphocyte subsets.
Abstract: A commercial bovine IFN-gamma-specific monoclonal antibody was used to measure antigen-specific IFN-gamma production by equine lymphocytes. Paired PBMC samples were collected from six ponies prior to and 10 days after challenge infection with equine herpesvirus-1 (EHV-1). Each sample was stimulated in vitro with EHV-1, virus-free medium, or PMA and ionomycin, and labelled with monoclonal antibodies specific for various equine lymphocyte subset markers. Following fixation, intracellular IFN-gamma was detected using a FITC-conjugated bovine IFN-gamma-specific monoclonal antibody. In vitro restimulation of PBMC with EHV-1 induced IFN-gamma production by a significantly higher percentage of total (CD5(+)) T lymphocytes, and CD4(+) and CD8(+) T lymphocyte subsets among post-EHV-1 infection PBMC samples compared to pre-infection samples. This response was associated with an increase in virus-specific CTL activity, a critical immune effector for the control of EHV-1 infection and disease. No significant increase in IFN-gamma production by B lymphocytes was observed. These data demonstrate that EHV-1 challenge infection of ponies results in increased production of IFN-gamma by virus-specific T lymphocytes, and that this response can be quantitated using flow cytometry.
Publication Date: 2004-12-29 PubMed ID: 15621307DOI: 10.1016/j.vetimm.2004.09.024Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The study investigates how infection with equine herpesvirus-1 (EHV-1) in ponies affects the production of a particular immune protein, IFN-gamma, in different types of T cells, a group of white blood cells crucial for immune responses.
Methodology
- A monoclonal antibody specific to bovine IFN-gamma was used to detect its production in equine, or horse, lymphocytes, which are white blood cells.
- Blood samples were withdrawn from six ponies before and ten days after experimental infection with EHV-1.
- Cultures from these samples were stimulated in the lab with either EHV-1, a virus-free medium as a control, or a powerful combination of cell stimulants.
- The cultures were then treated with antibodies specific to different markers on lymphocyte subsets, to distinguish between the different types of these cells.
- These antibodies were used to detect IFN-gamma within the cells.
Findings
- The researchers found a significant increase in IFN-gamma production in three types of T cells (total T lymphocytes, and CD4(+) and CD8(+) T lymphocyte subsets) in the samples taken following EHV-1 infection, compared to the pre-infection samples.
- The increase in IFN-gamma production was associated with a heightened activity of certain T cells called cytotoxic T lymphocytes, which are crucial for controlled EHV-1 infection.
- However, there was no significant rise in IFN-gamma production by B lymphocytes, another type of white blood cell.
Conclusion
- The study shows that infection with EHV-1 leads to a pronounced increase in the production of IFN-gamma by T lymphocytes, an immune response that can be measured using flow cytometry. This finding has potential implications for understanding the interplay between the immune system and viral infections in horses.
Cite This Article
APA
Breathnach CC, Soboll G, Suresh M, Lunn DP.
(2004).
Equine herpesvirus-1 infection induces IFN-gamma production by equine T lymphocyte subsets.
Vet Immunol Immunopathol, 103(3-4), 207-215.
https://doi.org/10.1016/j.vetimm.2004.09.024 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
MeSH Terms
- Animals
- Antibodies, Monoclonal / immunology
- Flow Cytometry / veterinary
- Herpesviridae Infections / immunology
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 1, Equid / immunology
- Horse Diseases / immunology
- Horse Diseases / virology
- Horses
- Interferon-gamma / biosynthesis
- Interferon-gamma / immunology
- Ionomycin / immunology
- Lymphocyte Activation / immunology
- Retrospective Studies
- T-Lymphocyte Subsets / immunology
- T-Lymphocyte Subsets / virology
- T-Lymphocytes, Cytotoxic / immunology
- Tetradecanoylphorbol Acetate / immunology
Citations
This article has been cited 14 times.- Schnabel CL, Fletemeyer B, Lübke S, Marti E, Wagner B, Alber G. CD154 Expression Indicates T Cell Activation Following Tetanus Toxoid Vaccination of Horses.. Front Immunol 2022;13:805026.
- Zarski LM, Weber PSD, Lee Y, Soboll Hussey G. Transcriptomic Profiling of Equine and Viral Genes in Peripheral Blood Mononuclear Cells in Horses during Equine Herpesvirus 1 Infection.. Pathogens 2021 Jan 7;10(1).
- Poelaert KCK, Van Cleemput J, Laval K, Favoreel HW, Couck L, Van den Broeck W, Azab W, Nauwynck HJ. Equine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs.. J Virol 2019 Apr 1;93(7).
- Wimer CL, Schnabel CL, Perkins G, Babasyan S, Freer H, Stout AE, Rollins A, Osterrieder N, Goodman LB, Glaser A, Wagner B. The deletion of the ORF1 and ORF71 genes reduces virulence of the neuropathogenic EHV-1 strain Ab4 without compromising host immunity in horses.. PLoS One 2018;13(11):e0206679.
- Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity.. BMC Vet Res 2018 Aug 22;14(1):245.
- Hue ES, Richard EA, Fortier CI, Fortier GD, Paillot R, Raue R, Pronost SL. Equine PBMC Cytokines Profile after In Vitro α- and γ-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment.. Vaccines (Basel) 2017 Sep 19;5(3).
- Liu SA, Stanfield BA, Chouljenko VN, Naidu S, Langohr I, Del Piero F, Ferracone J, Roy AA, Kousoulas KG. Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice.. J Virol 2017 Jun 15;91(12).
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- Lili Y, Yi W, Ji Y, Yue S, Weimin S, Ming L. Global activation of CD8+ cytotoxic T lymphocytes correlates with an impairment in regulatory T cells in patients with generalized vitiligo.. PLoS One 2012;7(5):e37513.
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