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Home / Equine Research Bank / Arch Virol / Equine herpesvirus type 1 (EHV-1) induced abortions and para...
Archives of virology1986; 90(3-4); 273-288; doi: 10.1007/BF01317376

Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a Lipizzaner stud: a contribution to the classification of equine herpesviruses.

Abstract: Out of 30 cases of abortion and perinatal deaths in a Lipizzaner stud in Austria 10 mares died after having shown central nervous system disturbances, ataxias and paralysis. The etiological agent of this "abortion storm" was equine herpesvirus type 1 (EHV-1). The restriction enzyme pattern of the DNA from 5 isolates recovered from fetuses has been analyzed and compared with the known reference strains of EHV-1, -2, -4 and an Austrian vaccine strain. The DNA restriction profiles of the Lipizzaner isolates as well as of the vaccine strain could be identified as being typical of abortigenic strains with minor variations. Such variations on the molecular biological level of the DNA do not justify characterization of the strains as neuro-variants. The vaccine strain differed from other isolates investigated with 4 restriction endonucleases (Bam HI, Bgl II, EcoRI, Kpn I) which was due to a deletion in the unique short segment of the genome. The lack of similar DNA bands in two EHV-1 viruses, causing mild respiratory disease, as well as in the vaccine strain Prevaccinol is suggestive of lowered virulence. In contrast to one Lipizzaner isolate tested (strain Austria IV) the Austrian vaccine strain proved to be of strong neurovirulence for suckling mice.
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Publication Date: 1986-01-01 PubMed ID: 3015084DOI: 10.1007/BF01317376Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a severe outbreak of equine herpesvirus type 1 (EHV-1) in a Lipizzaner stud in Austria. The researchers studied the DNA patterns from different isolates and compared them to known reference strains, finding that they were typical of abortigenic strains. They also investigated the vaccine strain and its potential virulence.

Outbreak of EHV-1 in a Lipizzaner Stud

  • The research focuses on an outbreak of EHV-1 among a Lipizzaner stud in Austria. The outbreak manifested in symptoms such as central nervous system disorders, ataxias, paralysis, and led to abortions and perinatal deaths.
  • Of the 30 cases observed, ten affected mares died following the presentation of these symptoms. The agent causing these fatal and abortion-inducing symptoms was identified as EHV-1.

DNA Analysis of EHV-1 Isolates

  • The herpesvirus was isolated from aborted fetuses, and an analysis of the DNA restriction patterns was conducted by the researchers.
  • This analysis was compared with known reference strains to EHV-1, EHV-2, EHV-4, and an Austrian vaccine strain.
  • It was determined that the DNA restriction patterns of the isolates in question were typical of abortigenic strains, with some minor variations. Despite these variations, the researchers indicated that the differences did not warrant classifying the strains as neuro-variants.

Examination of the Vaccine Strain

  • The vaccine strain studied was found to have differences with the other isolates, particularly with 4 restriction endonucleases (Bam HI, Bgl II, EcoRI, Kpn I). This is a consequence of a deletion in the genome’s unique short segment.
  • The fact that two other EHV-1 viruses causing mild respiratory disease, and the vaccine strain Prevaccinol, lacked similar DNA bands suggests a possibly reduced virulence in these variants. In contrast, one Lipizzaner isolate tested (strain Austria IV) showed a high degree of neurovirulence.
  • This unexpected finding reveals the need for further research into the vaccine, as despite it showing signs of lowered virulence compared to other strains, it still showed strong neurovirulence on suckling mice.

Cite This Article

APA
Chowdhury SI, Kubin G, Ludwig H. (1986). Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a Lipizzaner stud: a contribution to the classification of equine herpesviruses. Arch Virol, 90(3-4), 273-288. https://doi.org/10.1007/BF01317376

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 90
Issue: 3-4
Pages: 273-288

Researcher Affiliations

Chowdhury, S I
    Kubin, G
      Ludwig, H

        MeSH Terms

        • Abortion, Veterinary / microbiology
        • Animals
        • Chromosome Mapping
        • DNA Restriction Enzymes
        • DNA, Viral / genetics
        • Female
        • Herpesviridae / classification
        • Herpesviridae / genetics
        • Herpesviridae / pathogenicity
        • Horse Diseases / microbiology
        • Horses / microbiology
        • Male
        • Neurons / microbiology
        • Paralysis / microbiology
        • Pregnancy
        • Viral Vaccines

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        Citations

        This article has been cited 9 times.
        1. Kamel M, Pavulraj S, Fauler B, Mielke T, Azab W. Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells.. iScience 2020 Oct 23;23(10):101615.
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        2. Seeber PA, Quintard B, Sicks F, Dehnhard M, Greenwood AD, Franz M. Environmental stressors may cause equine herpesvirus reactivation in captive Grévy's zebras (Equus grevyi).. PeerJ 2018;6:e5422.
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        3. Romera SA, Puntel M, Quattrocchi V, Del Médico Zajac P, Zamorano P, Blanco Viera J, Carrillo C, Chowdhury S, Borca MV, Sadir AM. Protection induced by a glycoprotein E-deleted bovine herpesvirus type 1 marker strain used either as an inactivated or live attenuated vaccine in cattle.. BMC Vet Res 2014 Jan 8;10:8.
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