Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
Equine herpesvirus type 1 induces both neurological and respiratory disease in Syrian hamsters.
Abstract: The equine herpesvirus type 1 (EHV-1) is an important cause of myeloencephalopathy and respiratory disease in horses. Animal models for EHV-1 infection have been specially developed using mice and Syrian hamsters (Mesocricetus auratus). However, few studies have attempted to evaluate the pathogenesis of EHV-1 infection in the central nervous system (CNS) and respiratory system of hamsters. Therefore, the aim of this study was to evaluate the pathogenesis of four Brazilian EHV-1 strains within the CNS and lungs of Syrian hamsters. Hamsters intranasally infected with A4/72, A9/92, A3/97, and Iso/72 EHV-1 strains developed severe neurological and respiratory signs and died during acute EHV-1 infection within 3 to 5days post-inoculation. However, neurological signs were more severe in A4/72 and A9/92-infected hamsters, whereas respiratory signs were more prominent in A3/97 and Iso/72-infected hamsters. In the latter, lesions in the CNS were predominantly inflammatory, whereas in A4/72 and A9/92-infected hamsters, neuronal and liquefactive necrosis were the predominant lesions. EHV-1 infected hamsters also developed an interstitial pneumonia with infiltration of alveolar septa by macrophages, neutrophils, and lymphocytes, with the exception of A9/92-infected hamsters, which developed severe hemorrhages within the airways. EHV-1 antigens were detected along with CNS and pulmonary lesions. EHV-1 was also recovered from CNS of all infected hamsters, whereas the virus was recovered from the lungs of A4/72, A9/92, and Iso/72-infected hamsters. Brazilian EHV-1 strains caused both severe CNS and respiratory disease in hamsters, thus making this species an interesting model for EHV-1 infection in the CNS and respiratory system.
Copyright © 2017 Elsevier B.V. All rights reserved.
Publication Date: 2017-03-07 PubMed ID: 28619133DOI: 10.1016/j.vetmic.2017.03.007Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the pathogenic effects of the Equine herpesvirus type 1 (EHV-1) on the Central Nervous System (CNS) and respiratory system of Syrian hamsters. Specifically, the study assesses the impact of four different Brazilian EHV-1 strains and finds all tested strains result in both severe CNS and respiratory diseases.
Background
- The Equine herpesvirus type 1 (EHV-1) is a significant agent of myeloencephalopathy and respiratory disease in horses.
- Syrian hamsters have often been used as animal models to study the impact of EHV-1 infections.
- However, past research has seldom focused on exploring the pathogenesis of EHV-1 in the CNS and respiratory system of these hamsters.
Research Aim
- The study aims to provide an understanding of the pathogenic effects of four different Brazilian strains of EHV-1 on the CNS and lungs of Syrian hamsters.
Methodology and Results
- Hamsters were intranasally infected with one of the four EHV-1 strains: A4/72, A9/92, A3/97, and Iso/72.
- All infected hamsters developed severe neurological and respiratory signs and resulted in death within 3 to 5 days.
- The A4/72 and A9/92 strains caused more intense neurological signs, while the A3/97 and Iso/72 strains induced more severe respiratory symptoms.
- Hamsters infected with A3/97 and Iso/72 exhibited predominantly inflammatory lesions in the CNS, whereas those infected by A4/72 and A9/92 strains primarily developed neuronal and liquefactive necrosis.
- Interstitial pneumonia resulting from infiltration of macrophages, neutrophils, and lymphocytes was observed across the hamsters.
- Exceptionally, hamsters infected with the A9/92 strain experienced severe hemorrhaging within the airways.
- EHV-1 antigens were detected in the CNS and pulmonary lesions post-infection.
- The virus was successfully recovered from the CNS of all infected hamsters, and from the lungs of A4/72, A9/92, and Iso/72 infected hamsters.
Conclusion
- This research concluded that EHV-1 infection results in both serious CNS and respiratory diseases in Syrian hamsters, irrespective of the strain.
- As the study establishes the detrimental effects of EHV-1 strains on both the respiratory and CNS of Syrian hamsters, it likely paves the way for the animal becoming an important research model for future studies on EHV-1 infection.
Cite This Article
APA
Mesquita LP, Arévalo AF, Zanatto DA, Miyashiro SI, Cunha EMS, de Souza MDCC, Villalobos EMC, Mori CMC, Maiorka PC, Mori E.
(2017).
Equine herpesvirus type 1 induces both neurological and respiratory disease in Syrian hamsters.
Vet Microbiol, 203, 117-124.
https://doi.org/10.1016/j.vetmic.2017.03.007 Publication
Researcher Affiliations
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Biological Institute, Sao Paulo, Brazil.
- Biological Institute, Sao Paulo, Brazil.
- Biological Institute, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
- Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil; Pasteur Institute, Sao Paulo, Brazil. Electronic address: eniomori@gmail.com.
MeSH Terms
- Administration, Intranasal / veterinary
- Animals
- Cricetinae
- Herpesviridae Infections / pathology
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 1, Equid / pathogenicity
- Horse Diseases / pathology
- Horse Diseases / virology
- Horses / virology
- Male
- Mesocricetus
- Mice
- Nervous System Diseases / pathology
- Nervous System Diseases / veterinary
- Nervous System Diseases / virology
- Respiratory Tract Diseases / pathology
- Respiratory Tract Diseases / veterinary
- Respiratory Tract Diseases / virology
Citations
This article has been cited 8 times.- Hu Y, Jia Q, Liu J, Sun W, Bao Z, Che C, Wu G, Fan B, Jarhen, Ran D. Molecular characteristics and pathogenicity of an equid alphaherpesvirus 1 strain isolated in China. Virus Genes 2022 Aug;58(4):284-293.
- Wang T, Hu L, Liu M, Wang T, Hu X, Li Y, Liu W, Li Y, Wang Y, Ren H, Zhang W, Wang C, Li L. The Emergence of Viral Encephalitis in Donkeys by Equid Herpesvirus 8 in China. Front Microbiol 2022;13:840754.
- Warda FF, Ahmed HES, Shafik NG, Mikhael CA, Abd-ElAziz HMG, Mohammed WA, Shosha EA. Application of equine herpesvirus-1 vaccine inactivated by both formaldehyde and binary ethylenimine in equine. Vet World 2021 Jul;14(7):1815-1821.
- Ruan L, Li L, Yang R, You A, Khan MZ, Yu Y, Chen L, Li Y, Liu G, Wang C, Wang T. Equine Herpesvirus-1 Induced Respiratory Disease in Dezhou Donkey Foals: Case Study from China, 2024. Vet Sci 2025 Jan 14;12(1).
- Nishimura F, Fukushi N, Sakai H, Fukushi H. Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30. J Vet Med Sci 2024 Dec 1;86(12):1273-1278.
- Hu Y, Zhang SY, Sun WC, Feng YR, Gong HR, Ran DL, Zhang BZ, Liu JH. Breaking Latent Infection: How ORF37/38-Deletion Mutants Offer New Hope against EHV-1 Neuropathogenicity. Viruses 2024 Sep 16;16(9).
- Hu Y, Wu G, Jia Q, Zhang B, Sun W, Sa R, Zhang S, Cai W, Jarhen, Ran D, Liu J. Development of a live attenuated vaccine candidate for equid alphaherpesvirus 1 control: a step towards efficient protection. Front Immunol 2024;15:1408510.
- Li Z, He Y, Ge L, Quan R, Chen J, Hu Y, Sa R, Liu J, Ran D, Fu Q, Shi H. Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo. Front Vet Sci 2023;10:1163780.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
