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Home / Equine Research Bank / Infect Immun / Equine herpesviruses: antigenic relationships and deoxyribon...
Infection and immunity1973; 8(4); 621-627; doi: 10.1128/iai.8.4.621-627.1973

Equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities.

Abstract: Equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. Of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes formed a series of gradations with no clear hiatus which would permit the unequivocal delineation of the abortion viruses from the slowly growing strains. The 10 slowly growing isolates showed antigenic heterogeneity even though complement was present; the neutralizing capacity of an antiserum against the heterologous strains was, in most instances, markedly less than against the homologous strains, the range of the 50% endpoints being much greater than that observed among the equine abortion viruses, or among isolates of herpes simplex type 1. There was no cross neutralization between the equine abortion viruses and any of the 10 slowly growing isolates. An extra band of deoxyribonucleic acid, at 1.723 to 1.725 g/cm(3), was present in two of the slowly growing strains when originally grown in rabbit cells, but was no longer present after passage in cat cells. This band occupied the same position as one reported in the hamster-passaged strain of equine abortion virus, and had a density similar to that of the equine genital herpesvirus. Although the taxonomic demarcation of the equine abortion viruses and the slowly growing herpesviruses from one another is still open to question, they can be conveniently labeled equine herpesviruses 1 and 2, respectively; the genital virus would be termed equine herpesvirus 3.
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Publication Date: 1973-10-01 PubMed ID: 4742974PubMed Central: PMC422900DOI: 10.1128/iai.8.4.621-627.1973Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers compared characteristics of equine herpesviruses and found variations among different strains, leading them to propose a provisional labelling system.

Overview of the Study

  • The researchers compared equine herpesviruses with deoxyribonucleic acid (DNA) density of 1.716 to 1.717 g/cm(3). Comparison methods included the plaque-reduction test and the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures.
  • A total of 19 isolates were studied, of which 9 had been tentatively labeled as equine abortion viruses.

Findings

  • The equine abortion viruses grew more quickly than the other 10 isolates, and though there were gradations in mean plaque sizes, there was no clear delineation to unequivocally identify these viruses based on plaque sizes.
  • Among the 10 slower-growing isolates, there was an observed antigenic heterogeneity even when complement was present. However, the neutralizing capacity of antiserum was markedly reduced against heterologous (differing in characteristics) strains compared to homologous strains.
  • There was no cross neutralization observed between the equine abortion viruses and any of the slowly growing isolates indicating a strong distinguishing feature between these groups.

Notable Observations

  • An extra band of DNA, was noted in two of the slowly growing strains when originally grown in rabbit cells, but it disappeared after passage in cat cells. This band’s density was similar to the equine genital herpesvirus density and occupied the same position as the one reported in the hamster-passaged strain of equine abortion virus.
  • Despite these observations, the differentiation between equine abortion viruses and slowly growing herpesviruses remained uncertain, leading the researchers to suggest a temporary naming convention.

Suggested Naming Convention

  • The researchers proposed that the equine abortion viruses can be conveniently labeled as equine herpesviruses 1 and the slower growing herpesviruses as equine herpesviruses 2. They suggested the genital virus be referred to as equine herpesvirus 3.

Cite This Article

APA
Plummer G, Goodheart CR, Studdert MJ. (1973). Equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities. Infect Immun, 8(4), 621-627. https://doi.org/10.1128/iai.8.4.621-627.1973

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 8
Issue: 4
Pages: 621-627

Researcher Affiliations

Plummer, G
    Goodheart, C R
      Studdert, M J

        MeSH Terms

        • Animals
        • Antigens, Viral / analysis
        • Cytopathogenic Effect, Viral
        • DNA, Viral / analysis
        • Densitometry
        • Herpesviridae / immunology
        • Horses / immunology
        • Immune Sera
        • Kidney / microbiology
        • Neutralization Tests
        • Rabbits / immunology
        • Viral Plaque Assay
        • Virus Cultivation

        References

        This article includes 21 references
        1. Hsiung GD, Fischman HR, Fong CK, Green RH. Characterization of a cytomegalo-like virus isolated from spontaneously degenerated equine kidney cell culture.. Proc Soc Exp Biol Med 1969 Jan;130(1):80-4.
          pubmed: 4302962doi: 10.3181/00379727-130-33492google scholar: lookup
        2. Studdert MJ, Turner AJ, Peterson JE. Equine herpesviruses. I. Isolation and characterisation of equine rhinopneumonitis virus and other equine herpesviruses from horses.. Aust Vet J 1970 Mar;46(3):83-9.
          pubmed: 4317854doi: 10.1111/j.1751-0813.1970.tb15927.xgoogle scholar: lookup
        3. Andersen HK. Studies of human cytomegalovirus strain variations by kinetic neutralization tests.. Arch Gesamte Virusforsch 1972;38(4):297-305.
          pubmed: 4343371doi: 10.1007/BF01262820google scholar: lookup
        4. RUSSELL WC, CRAWFORD LV. PROPERTIES OF THE NUCLEIC ACIDS FROM SOME HERPES GROUP VIRUSES.. Virology 1964 Feb;22:288-92.
          pubmed: 14165337doi: 10.1016/0042-6822(64)90017-0google scholar: lookup
        5. PLUMMER G, WATERSON AP. Equine herpes viruses.. Virology 1963 Mar;19:412-6.
          pubmed: 13944111doi: 10.1016/0042-6822(63)90083-7google scholar: lookup
        6. Karpas A. Characterization of a new herpes-like virus isolated from foal kidney.. Ann Inst Pasteur (Paris) 1966 May;110(5):688-96.
          pubmed: 5909615
        7. Birnbaum G, Lynch JI, Margileth AM, Lonergan WM, Sever JL. Cytomegalovirus infections in newborn infants.. J Pediatr 1969 Nov;75(5):789-95.
          pubmed: 4310468doi: 10.1016/s0022-3476(69)80301-xgoogle scholar: lookup
        8. Hampar B, Martos LM, Ablashi DV, Siguënza RF, Wells GA. Differentiation of cross-reacting simian cytomegalovirus strains by late 19S rabbit neutralizing antibodies.. J Immunol 1969 Nov;103(5):1155-6.
          pubmed: 4310839
        9. Dreesman GR, Benyesh-Melnick M. Spectrum of human cytomegalovirus complement-fixing antigens.. J Immunol 1967 Dec;99(6):1106-14.
          pubmed: 4293994
        10. Ludwig H, Biswal N, Bryans JT, McCombs RM. Some properties of the DNA from a new equine herpesvirus.. Virology 1971 Aug;45(2):534-7.
          pubmed: 4106354doi: 10.1016/0042-6822(71)90357-6google scholar: lookup
        11. DOLL ER, MCCOLLUM WH, BRYANS JW, CROWE EW. Propagation of equine abortion virus in the embryonated chicken egg.. Cornell Vet 1956 Jan;46(1):97-108.
          pubmed: 13277328
        12. Pascoe RR, Spradbrow PB, Bagust TJ. An equine genital infection resembling coital exanthema associated with a virus.. Aust Vet J 1969 Apr;45(4):166-70.
          pubmed: 4305974doi: 10.1111/j.1751-0813.1969.tb01922.xgoogle scholar: lookup
        13. Turner AJ, Studdert MJ, Peterson JE. Equine herpes viruses. 2. Persistence of equine herpesviruses in experimentally infected horses and the experimental induction of abortion.. Aust Vet J 1970 Mar;46(3):90-8.
          pubmed: 4316594doi: 10.1111/j.1751-0813.1970.tb15928.xgoogle scholar: lookup
        14. Goodheart CR, Plummer G, Waner JL. Density difference of DNA of human herpes simplex viruses, types I and II.. Virology 1968 Jul;35(3):473-5.
          pubmed: 4298652doi: 10.1016/0042-6822(68)90225-0google scholar: lookup
        15. Andersen HK. Serologic differentiation of human cytomegalovirus strains using rabbit hyperimmune sera. Brief report.. Arch Gesamte Virusforsch 1971;33(1):187-91.
          pubmed: 4325973doi: 10.1007/BF01254177google scholar: lookup
        16. MEDEARIS DN Jr. OBSERVATIONS CONCERNING HUMAN CYTOMEGALOVIRUS INFECTION AND DISEASE.. Bull Johns Hopkins Hosp 1964 Mar;114:181-211.
          pubmed: 14130485
        17. Haines HG, Von Essen R, Benyesh-Melnick M, Melnick JL. Preparation of specific antisera to cytomegaloviruses in goats.. Proc Soc Exp Biol Med 1971 Dec;138(3):846-9.
          pubmed: 4108789doi: 10.3181/00379727-138-36003google scholar: lookup
        18. Plummer G, Bowling CP, Goodheart CR. Comparison of four horse herpesviruses.. J Virol 1969 Nov;4(5):738-41.
          pubmed: 4982831doi: 10.1128/JVI.4.5.738-741.1969google scholar: lookup
        19. WELLER TH, HANSHAW JB, SCOTT DE. Serologic differentiation of viruses responsible for cytomegalic inclusion disease.. Virology 1960 Sep;12:130-2.
          pubmed: 13843861doi: 10.1016/0042-6822(60)90156-2google scholar: lookup
        20. DOLL ER, BRYANS JT. Incubation periods for abortion in equine viral rhinopneumonitis.. J Am Vet Med Assoc 1962 Aug 1;140:351-4.
          pubmed: 13887042
        21. HANSHAW JB, BETTS RF, SIMON G, BOYNTON RC. ACQUIRED CYTOMEGALOVIRUS INFECTION: ASSOCIATION WITH HEPATOMEGALY AND ABNORMAL LIVER-FUNCTION TESTS.. N Engl J Med 1965 Mar 25;272:602-9.
          pubmed: 14255334doi: 10.1056/NEJM196503252721202google scholar: lookup

        Citations

        This article has been cited 15 times.
        1. Badr C, Souiai O, Arbi M, El Behi I, Essaied MS, Khosrof I, Benkahla A, Chabchoub A, Ghram A. Epidemiological and Phylogeographic Study of Equid Herpesviruses in Tunisia. Pathogens 2022 Sep 5;11(9).
          doi: 10.3390/pathogens11091016pubmed: 36145448google scholar: lookup
        2. Onasanya AE, El-Hage C, Diaz-Méndez A, Vaz PK, Legione AR, Browning GF, Devlin JM, Hartley CA. Whole genome sequence analysis of equid gammaherpesvirus -2 field isolates reveals high levels of genomic diversity and recombination. BMC Genomics 2022 Aug 30;23(1):622.
          doi: 10.1186/s12864-022-08789-xpubmed: 36042397google scholar: lookup
        3. Anis E, Ilha MRS, Engiles JB, Wilkes RP. Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples. J Vet Diagn Invest 2021 Mar;33(2):227-234.
          doi: 10.1177/1040638720978381pubmed: 33305693google scholar: lookup
        4. Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E, Torsteinsdóttir S, Svansson V. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response. PLoS One 2019;14(6):e0218576.
          doi: 10.1371/journal.pone.0218576pubmed: 31226153google scholar: lookup
        5. Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review. Vet Med (Auckl) 2015;6:91-101.
          doi: 10.2147/VMRR.S39473pubmed: 30155436google scholar: lookup
        6. Craig MI, Barrandeguy ME, Fernández FM. Equine herpesvirus 2 (EHV-2) infection in thoroughbred horses in Argentina. BMC Vet Res 2005 Nov 9;1:9.
          doi: 10.1186/1746-6148-1-9pubmed: 16281971google scholar: lookup
        7. Kamada M, Studdert MJ. Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Brief report. Arch Virol 1983;77(2-4):259-64.
          doi: 10.1007/BF01309273pubmed: 6314939google scholar: lookup
        8. Studdert MJ. Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. Arch Virol 1983;77(2-4):249-58.
          doi: 10.1007/BF01309272pubmed: 6314938google scholar: lookup
        9. Browning GF, Studdert MJ. Epidemiology of equine herpesvirus 2 (equine cytomegalovirus). J Clin Microbiol 1987 Jan;25(1):13-6.
          doi: 10.1128/jcm.25.1.13-16.1987pubmed: 3025249google scholar: lookup
        10. Chowdhury SI, Kubin G, Ludwig H. Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a Lipizzaner stud: a contribution to the classification of equine herpesviruses. Arch Virol 1986;90(3-4):273-88.
          doi: 10.1007/BF01317376pubmed: 3015084google scholar: lookup
        11. Browning GF, Studdert MJ. Physical mapping of a genome of equine herpesvirus 2 (equine cytomegalovirus). Arch Virol 1989;104(1-2):77-86.
          doi: 10.1007/BF01313809pubmed: 2923549google scholar: lookup
        12. Browning GF, Studdert MJ. Physical mapping of the genomic heterogeneity of isolates of equine herpesvirus 2 (equine cytomegalovirus). Arch Virol 1989;104(1-2):87-94.
          doi: 10.1007/BF01313810pubmed: 2564271google scholar: lookup
        13. Staczek J. Animal cytomegaloviruses. Microbiol Rev 1990 Sep;54(3):247-65.
          doi: 10.1128/mr.54.3.247-265.1990pubmed: 2170830google scholar: lookup
        14. Roizmann B, Desrosiers RC, Fleckenstein B, Lopez C, Minson AC, Studdert MJ. The family Herpesviridae: an update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses. Arch Virol 1992;123(3-4):425-49.
          doi: 10.1007/BF01317276pubmed: 1562239google scholar: lookup
        15. Gutekunst DE, Malmquist WA, Becvar CS. Antigenic relatedness of equine herpes virus types 1 and 3. Arch Virol 1978;56(1-2):33-45.
          doi: 10.1007/BF01317281pubmed: 75724google scholar: lookup
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