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Zentralblatt fur Veterinarmedizin. Reihe A1976; 23(6); 479-484; doi: 10.1111/j.1439-0442.1976.tb01729.x

Equine infectious anemia. Pyruvate kinase deficiency in the red cells of thoroughbred horses.

Abstract: Summary: The authors studied glycolytic and non-glycolytic erythrocytic enzyme activities in 8 thoroughbred horses with equine infectious anemia (EIA) and 16 normal controls. Biochemical lesions were indicated, the most outstanding being a deficiency of pyruvatekinase. Adenvlatekinase could be considered as a “salvage pathway” for the formation of ATP. Zusammenfassung: Infektiöse Anemie bei Pferden Mangel an Pirovatokinase in Erythrocyten der Englischen Vollblutpferde Die Autoren untersuchten die Aktivität der glykolytischen und der nichtglykolytischen Enzyme der Erythrocyten von zehn normalen englischen Vollblutpferden und acht Pferden mit infektiöser Anemie. Die biochemischen Schäden wurden festgestellt. Der Hauptschaden ist Mangel an Pyruvatkinase. Die Adenylatkinase kann als „Rettungsweg” für die Bildung von ATP angesehen werden. Résumé: Anémie infectieuse équine Deficience de pyruvatekinase chez les érythrocytes des chevaux pur sang anglais Les auteurs ont étudié la activité des enzymes glycolytiques et non glycolitiques chez les érythrocytes de 16 chevaux pur sang anglais normales et de 8 chevaux atteints d'anémie infectieuse. Les lesions biochimiques ont été mises en evidence et le point plus important est la déficience de pyruvatekinase. L'adenylatekinase peut être consideré comme la «voie de sauvetage» pour la formation d'ATP. Resumen: Anemia infecciosa equina Deficiencia de la piruvatoquinasa en los eritrocitos de equinos pura sangre de carrera Los autores estudiaron la actividad de las enzimas glucolíticas y no glucolíticas de los eritrocitos de 16 equinos pura sangre normales y de 8 acometidos de anemia infecciosa. Las lesiones bioquímicas fueron discutidas y el punto más importante es la deficiencia de piruvatoquinasa. La adenilatoquinasa puede ser considerada la «vía de salvación» para la formacion de ATP.
Publication Date: 1976-08-01 PubMed ID: 824904DOI: 10.1111/j.1439-0442.1976.tb01729.xGoogle Scholar: Lookup
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  • Journal Article

Summary

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This study investigates specific enzyme activities in the blood cells of thoroughbred horses suffering from equine infectious anemia (EIA), revealing a deficiency in the enzyme pyruvate kinase.

Research Aim and Methodology

  • The objective was to examine the enzyme activities in erythrocytes, specifically glycolytic (relating to or caused by metabolism that generates ATP through the breakdown of glucose) and non-glycolytic, in horses suffering from equine infectious anemia (EIA).
  • The sample set included 8 thoroughbred horses diagnosed with EIA and 16 healthy control horses.

Findings

  • The study revealed biochemical irregularities in the afflicted horses, and the most significant finding was the deficiency of the enzyme pyruvate kinase in the blood cells of horses suffering from EIA.
  • Pyruvate kinase’s primary function involves the final step of glycolysis (the breakdown of glucose into ATP), where it aids in the conversion of phosphoenolpyruvate into pyruvate. This process generates energy for the cell in the form of ATP. Hence, the deficiency of this enzyme in EIA horses could lead to disrupted energy production in the erythrocytes.

Secondary Findings and Further Discussion

  • Adenylate kinase, another enzyme, was indicated as a potential ‘salvage pathway’ for the formation of ATP. This means it could have a compensatory role in ensuring the erythrocytes still generate ATP, even when pyruvate kinase is lacking.
  • These findings show significant biochemical changes in the red blood cells of thoroughbred horses with EIA, notably the deficiency of the pyruvate kinase enzyme. This provides a crucial understanding of the pathophysiology of EIA and potentially informs future therapeutic strategies for EIA in horses.

Cite This Article

APA
Medeiros LO, Medeiros LF, Barcelos SR, Ferri S, Reiner UR. (1976). Equine infectious anemia. Pyruvate kinase deficiency in the red cells of thoroughbred horses. Zentralbl Veterinarmed A, 23(6), 479-484. https://doi.org/10.1111/j.1439-0442.1976.tb01729.x

Publication

ISSN: 0514-7158
NlmUniqueID: 0331323
Country: Germany
Language: English
Volume: 23
Issue: 6
Pages: 479-484

Researcher Affiliations

Medeiros, L O
    Medeiros, L F
      Barcelos, S R
        Ferri, S
          Reiner, U R

            MeSH Terms

            • Animals
            • Equine Infectious Anemia / enzymology
            • Erythrocytes / enzymology
            • Glycolysis
            • Horses
            • Pyruvate Kinase / deficiency

            Citations

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