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Home / Equine Research Bank / J Virol / Equine Infectious Anemia Virus Gag Assembly and Export Are D...
Journal of virology2015; 90(4); 1824-1838; doi: 10.1128/JVI.02814-15

Equine Infectious Anemia Virus Gag Assembly and Export Are Directed by Matrix Protein through trans-Golgi Networks and Cellular Vesicles.

Abstract: Gag intracellular assembly and export are very important processes for lentiviruses replication. Previous studies have demonstrated that equine infectious anemia virus (EIAV) matrix (MA) possesses distinct phosphoinositide affinity compared with HIV-1 MA and that phosphoinositide-mediated targeting to peripheral and internal membranes is a critical factor in EIAV assembly and release. In this study, we compared the cellular assembly sites of EIAV and HIV-1. We observed that the assembly of EIAV particles occurred on interior cellular membranes, while HIV-1 was targeted to the plasma membrane (PM) for assembly. Then, we determined that W7 and K9 in the EIAV MA N terminus were essential for Gag assembly and release but did not affect the cellular distribution of Gag. The replacement of EIAV MA with HIV-1 MA directed chimeric Gag to the PM but severely impaired Gag release. MA structural analysis indicated that the EIAV and HIV-1 MAs had similar spatial structures but that helix 1 of the EIAV MA was closer to loop 2. Further investigation indicated that EIAV Gag accumulated in the trans-Golgi network (TGN) but not the early and late endosomes. The 9 N-terminal amino acids of EIAV MA harbored the signal that directed Gag to the TGN membrane system. Additionally, we demonstrated that EIAV particles were transported to the extracellular space by the cellular vesicle system. This type of EIAV export was not associated with multivesicular bodies or microtubule depolymerization but could be inhibited by the actin-depolymerizing drug cytochalasin D, suggesting that dynamic actin depolymerization may be associated with EIAV production. Objective: In previous studies, EIAV Gag was reported to localize to both the cell interior and the plasma membrane. Here, we demonstrate that EIAV likely uses the TGN as the assembly site in contrast to HIV-1, which is targeted to the PM for assembly. These distinct assembly features are determined by the MA domain. We also identified two sites in the N terminus of EIAV MA that were important for Gag assembly and release. Furthermore, the observation of EIAV transport by cellular vesicles but not by multivesicular bodies sheds light on the mechanisms underlying EIAV cellular replication.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
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Publication Date: 2015-12-04 PubMed ID: 26637458PubMed Central: PMC4734017DOI: 10.1128/JVI.02814-15Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on understanding the unique replication process of the Equine Infectious Anemia Virus (EIAV) at a cellular level, particularly how it assembles and exports its proteins, and how it differs from the HIV-1 virus in this regard.

Objective

The researchers compare the EIAV with HIV-1 with respect to their cellular assembly and export sites. Their objective is to highlight the differences in assembly between the two viruses and reveal the specificities in cellular replication of the EIAV.

Assembly Sites of EIAV and HIV-1

  • While HIV-1 targets the plasma membrane (PM), the study found that EIAV particles assemble on internal cellular membranes.

Importance of Phosphoinositide Affinity in EIAV “Matrix (MA)”

  • The research underscores the differing phosphoinositide affinities between EIAV MA and HIV-1 MA and how it plays a vital role in the assembly and release of EIAV.

Role of W7 and K9 in Gag Assembly and Release

  • The researchers found that amino acid residues W7 and K9 in EIAV MA’s N terminus are crucial for Gag assembly and release, but do not affect Gag’s cellular distribution.
  • Substituting EIAV MA with HIV-1 MA directed the chimeric Gag to the PM but severely impeded Gag release.

Spatial Structure Differences Between EIAV and HIV-1 MA

  • Although HIV-1 MA and EIAV MA are similar in spatial structure, helix 1 of the EIAV MA was observed closer to loop 2.

EIAV Gag’s Localization in Trans-Golgi Network (TGN)

  • EIAV Gag was shown to aggregate in the TGN, rather than early and late endosomes.
  • The signal directing Gag to the TGN membrane system was found within the 9 N-terminal amino acids of EIAV MA.

Export of EIAV Particles

  • The study demonstrated that EIAV particles are transported to the extracellular space via the cellular vesicle system.
  • This type of EIAV export is independent of multivesicular bodies involvement or microtubule depolymerization; however, it can be inhibited by the actin-depolymerizing drug cytochalasin D, suggesting a possible connection between dynamic actin depolymerization and EIAV production.

Conclusion

The researchers discovered distinct cellular mechanisms of assembly and export of EIAV in comparison to HIV-1. These findings can enhance our understanding of how lentiviruses replicate and potentially lead to the development of more effective treatments for diseases caused by such viruses.

Cite This Article

APA
Zhang Z, Ma J, Zhang X, Su C, Yao QC, Wang X. (2015). Equine Infectious Anemia Virus Gag Assembly and Export Are Directed by Matrix Protein through trans-Golgi Networks and Cellular Vesicles. J Virol, 90(4), 1824-1838. https://doi.org/10.1128/JVI.02814-15

Publication

Journal of virology
ISSN: 1098-5514
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 90
Issue: 4
Pages: 1824-1838

Researcher Affiliations

Zhang, Zeli
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China.
Ma, Jian
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China.
Zhang, Xiang
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China.
Su, Chao
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China.
Yao, Qiu-Cheng
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China.
Wang, Xiaojun
  • State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Sciences, Harbin, Heilongjiang, China xjw@hvri.ac.cn.

MeSH Terms

  • Cytoplasmic Vesicles / metabolism
  • Gene Products, gag / metabolism
  • HIV-1 / physiology
  • Humans
  • Infectious Anemia Virus, Equine / physiology
  • Protein Transport
  • Viral Matrix Proteins / metabolism
  • Virus Assembly
  • trans-Golgi Network / metabolism

References

This article includes 49 references
  1. Leroux C, Cadoré JL, Montelaro RC. Equine Infectious Anemia Virus (EIAV): what has HIV's country cousin got to tell us?. Vet Res 2004 Jul-Aug;35(4):485-512.
    doi: 10.1051/vetres:2004020pubmed: 15236678google scholar: lookup
  2. Nagarajan MM, Simard C. Gag genetic heterogeneity of equine infectious anemia virus (EIAV) in naturally infected horses in Canada.. Virus Res 2007 Nov;129(2):228-35.
    doi: 10.1016/j.virusres.2007.07.013pubmed: 17767972google scholar: lookup
  3. Stephens RM, Casey JW, Rice NR. Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus.. Science 1986 Feb 7;231(4738):589-94.
    doi: 10.1126/science.3003905pubmed: 3003905google scholar: lookup
  4. Bieniasz PD. The cell biology of HIV-1 virion genesis.. Cell Host Microbe 2009 Jun 18;5(6):550-8.
    doi: 10.1016/j.chom.2009.05.015pmc: PMC3736989pubmed: 19527882google scholar: lookup
  5. Chen C, Vincent O, Jin J, Weisz OA, Montelaro RC. Functions of early (AP-2) and late (AIP1/ALIX) endocytic proteins in equine infectious anemia virus budding.. J Biol Chem 2005 Dec 9;280(49):40474-80.
    doi: 10.1074/jbc.M509317200pubmed: 16215227google scholar: lookup
  6. Puffer BA, Watkins SC, Montelaro RC. Equine infectious anemia virus Gag polyprotein late domain specifically recruits cellular AP-2 adapter protein complexes during virion assembly.. J Virol 1998 Dec;72(12):10218-21.
    pmc: PMC110572pubmed: 9811764doi: 10.1128/JVI.72.12.10218-10221.1998google scholar: lookup
  7. Tanzi GO, Piefer AJ, Bates P. Equine infectious anemia virus utilizes host vesicular protein sorting machinery during particle release.. J Virol 2003 Aug;77(15):8440-7.
    doi: 10.1128/JVI.77.15.8440-8447.2003pmc: PMC165230pubmed: 12857913google scholar: lookup
  8. Bieniasz PD. Late budding domains and host proteins in enveloped virus release.. Virology 2006 Jan 5;344(1):55-63.
    doi: 10.1016/j.virol.2005.09.044pubmed: 16364736google scholar: lookup
  9. Jouvenet N, Zhadina M, Bieniasz PD, Simon SM. Dynamics of ESCRT protein recruitment during retroviral assembly.. Nat Cell Biol 2011 Apr;13(4):394-401.
    doi: 10.1038/ncb2207pmc: PMC3245320pubmed: 21394083google scholar: lookup
  10. Saad JS, Loeliger E, Luncsford P, Liriano M, Tai J, Kim A, Miller J, Joshi A, Freed EO, Summers MF. Point mutations in the HIV-1 matrix protein turn off the myristyl switch.. J Mol Biol 2007 Feb 16;366(2):574-85.
    doi: 10.1016/j.jmb.2006.11.068pmc: PMC1853300pubmed: 17188710google scholar: lookup
  11. Zhou W, Parent LJ, Wills JW, Resh MD. Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids.. J Virol 1994 Apr;68(4):2556-69.
    pmc: PMC236733pubmed: 8139035doi: 10.1128/JVI.68.4.2556-2569.1994google scholar: lookup
  12. Chukkapalli V, Hogue IB, Boyko V, Hu WS, Ono A. Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane binding.. J Virol 2008 Mar;82(5):2405-17.
    doi: 10.1128/JVI.01614-07pmc: PMC2258911pubmed: 18094158google scholar: lookup
  13. Hatanaka H, Iourin O, Rao Z, Fry E, Kingsman A, Stuart DI. Structure of equine infectious anemia virus matrix protein.. J Virol 2002 Feb;76(4):1876-83.
    doi: 10.1128/JVI.76.4.1876-1883.2002pmc: PMC135893pubmed: 11799182google scholar: lookup
  14. Chen K, Bachtiar I, Piszczek G, Bouamr F, Carter C, Tjandra N. Solution NMR characterizations of oligomerization and dynamics of equine infectious anemia virus matrix protein and its interaction with PIP2.. Biochemistry 2008 Feb 19;47(7):1928-37.
    doi: 10.1021/bi701984hpmc: PMC3120113pubmed: 18220420google scholar: lookup
  15. Fernandes F, Chen K, Ehrlich LS, Jin J, Chen MH, Medina GN, Symons M, Montelaro R, Donaldson J, Tjandra N, Carter CA. Phosphoinositides direct equine infectious anemia virus gag trafficking and release.. Traffic 2011 Apr;12(4):438-51.
    doi: 10.1111/j.1600-0854.2010.01153.xpmc: PMC3064743pubmed: 21176037google scholar: lookup
  16. Kutluay SB, Zang T, Blanco-Melo D, Powell C, Jannain D, Errando M, Bieniasz PD. Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis.. Cell 2014 Nov 20;159(5):1096-1109.
    doi: 10.1016/j.cell.2014.09.057pmc: PMC4247003pubmed: 25416948google scholar: lookup
  17. Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist WI. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.. Cell 2001 Oct 5;107(1):55-65.
    doi: 10.1016/S0092-8674(01)00506-2pubmed: 11595185google scholar: lookup
  18. Martin-Serrano J, Zang T, Bieniasz PD. HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress.. Nat Med 2001 Dec;7(12):1313-9.
    doi: 10.1038/nm1201-1313pubmed: 11726971google scholar: lookup
  19. Kikonyogo A, Bouamr F, Vana ML, Xiang Y, Aiyar A, Carter C, Leis J. Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cells.. Proc Natl Acad Sci U S A 2001 Sep 25;98(20):11199-204.
    doi: 10.1073/pnas.201268998pmc: PMC58707pubmed: 11562473google scholar: lookup
  20. Li F, Chen C, Puffer BA, Montelaro RC. Functional replacement and positional dependence of homologous and heterologous L domains in equine infectious anemia virus replication.. J Virol 2002 Feb;76(4):1569-77.
    doi: 10.1128/JVI.76.4.1569-1577.2002pmc: PMC135910pubmed: 11799151google scholar: lookup
  21. Ott DE, Coren LV, Gagliardi TD, Nagashima K. Heterologous late-domain sequences have various abilities to promote budding of human immunodeficiency virus type 1.. J Virol 2005 Jul;79(14):9038-45.
    doi: 10.1128/JVI.79.14.9038-9045.2005pmc: PMC1168796pubmed: 15994797google scholar: lookup
  22. Jouvenet N, Bieniasz PD, Simon SM. Imaging the biogenesis of individual HIV-1 virions in live cells.. Nature 2008 Jul 10;454(7201):236-40.
    doi: 10.1038/nature06998pmc: PMC2708942pubmed: 18500329google scholar: lookup
  23. Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD. Plasma membrane is the site of productive HIV-1 particle assembly.. PLoS Biol 2006 Dec;4(12):e435.
    doi: 10.1371/journal.pbio.0040435pmc: PMC1750931pubmed: 17147474google scholar: lookup
  24. Houzet L, Gay B, Morichaud Z, Briant L, Mougel M. Intracellular assembly and budding of the Murine Leukemia Virus in infected cells.. Retrovirology 2006 Feb 10;3:12.
    doi: 10.1186/1742-4690-3-12pmc: PMC1434767pubmed: 16472393google scholar: lookup
  25. Pelchen-Matthews A, Kramer B, Marsh M. Infectious HIV-1 assembles in late endosomes in primary macrophages.. J Cell Biol 2003 Aug 4;162(3):443-55.
    doi: 10.1083/jcb.200304008pmc: PMC2172706pubmed: 12885763google scholar: lookup
  26. Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W. Visualization of retroviral replication in living cells reveals budding into multivesicular bodies.. Traffic 2003 Nov;4(11):785-801.
    doi: 10.1034/j.1600-0854.2003.00135.xpubmed: 14617360google scholar: lookup
  27. Ott DE, Coren LV, Sowder RC 2nd, Adams J, Nagashima K, Schubert U. Equine infectious anemia virus and the ubiquitin-proteasome system.. J Virol 2002 Mar;76(6):3038-44.
    doi: 10.1128/JVI.76.6.3038-3044.2002pmc: PMC135975pubmed: 11861870google scholar: lookup
  28. Voeltz GK, Rolls MM, Rapoport TA. Structural organization of the endoplasmic reticulum.. EMBO Rep 2002 Oct;3(10):944-50.
    doi: 10.1093/embo-reports/kvf202pmc: PMC1307613pubmed: 12370207google scholar: lookup
  29. Berka U, Hamann MV, Lindemann D. Early events in foamy virus-host interaction and intracellular trafficking.. Viruses 2013 Apr 8;5(4):1055-74.
    doi: 10.3390/v5041055pmc: PMC3705265pubmed: 23567621google scholar: lookup
  30. Bryant M, Ratner L. Myristoylation-dependent replication and assembly of human immunodeficiency virus 1.. Proc Natl Acad Sci U S A 1990 Jan;87(2):523-7.
    doi: 10.1073/pnas.87.2.523pmc: PMC53297pubmed: 2405382google scholar: lookup
  31. Chukkapalli V, Ono A. Molecular determinants that regulate plasma membrane association of HIV-1 Gag.. J Mol Biol 2011 Jul 22;410(4):512-24.
    doi: 10.1016/j.jmb.2011.04.015pmc: PMC3139151pubmed: 21762797google scholar: lookup
  32. Tedbury PR, Mercredi PY, Gaines CR, Summers MF, Freed EO. Elucidating the mechanism by which compensatory mutations rescue an HIV-1 matrix mutant defective for gag membrane targeting and envelope glycoprotein incorporation.. J Mol Biol 2015 Mar 27;427(6 Pt B):1413-1427.
    doi: 10.1016/j.jmb.2015.01.018pmc: PMC4844178pubmed: 25659909google scholar: lookup
  33. Pacyniak E, Gomez ML, Gomez LM, Mulcahy ER, Jackson M, Hout DR, Wisdom BJ, Stephens EB. Identification of a region within the cytoplasmic domain of the subtype B Vpu protein of human immunodeficiency virus type 1 (HIV-1) that is responsible for retention in the golgi complex and its absence in the Vpu protein from a subtype C HIV-1.. AIDS Res Hum Retroviruses 2005 May;21(5):379-94.
    doi: 10.1089/aid.2005.21.379pubmed: 15929700google scholar: lookup
  34. Ma J, Wang SS, Lin YZ, Liu HF, Wei HM, Du C, Wang XF, Zhou JH. An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages.. Vet Microbiol 2013 Sep 27;166(1-2):263-9.
    doi: 10.1016/j.vetmic.2013.06.005pubmed: 23850441google scholar: lookup
  35. Ma J, Shi N, Jiang CG, Lin YZ, Wang XF, Wang S, Lv XL, Zhao LP, Shao YM, Kong XG, Zhou JH, Shen RX. A proviral derivative from a reference attenuated EIAV vaccine strain failed to elicit protective immunity.. Virology 2011 Feb 5;410(1):96-106.
    doi: 10.1016/j.virol.2010.10.032pubmed: 21094511google scholar: lookup
  36. Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M. Ceramide triggers budding of exosome vesicles into multivesicular endosomes.. Science 2008 Feb 29;319(5867):1244-7.
    doi: 10.1126/science.1153124pubmed: 18309083google scholar: lookup
  37. Cocucci E, Racchetti G, Meldolesi J. Shedding microvesicles: artefacts no more.. Trends Cell Biol 2009 Feb;19(2):43-51.
    doi: 10.1016/j.tcb.2008.11.003pubmed: 19144520google scholar: lookup
  38. Aniento F, Emans N, Griffiths G, Gruenberg J. Cytoplasmic dynein-dependent vesicular transport from early to late endosomes.. J Cell Biol 1993 Dec;123(6 Pt 1):1373-87.
    doi: 10.1083/jcb.123.6.1373pmc: PMC2290907pubmed: 8253838google scholar: lookup
  39. Lebrand C, Corti M, Goodson H, Cosson P, Cavalli V, Mayran N, Fauré J, Gruenberg J. Late endosome motility depends on lipids via the small GTPase Rab7.. EMBO J 2002 Mar 15;21(6):1289-300.
    doi: 10.1093/emboj/21.6.1289pmc: PMC125356pubmed: 11889035google scholar: lookup
  40. Numrich J, Ungermann C. Endocytic Rabs in membrane trafficking and signaling.. Biol Chem 2014 Mar;395(3):327-33.
    pubmed: 24158421doi: 10.1515/hsz-2013-0258google scholar: lookup
  41. Vanlandingham PA, Ceresa BP. Rab7 regulates late endocytic trafficking downstream of multivesicular body biogenesis and cargo sequestration.. J Biol Chem 2009 May 1;284(18):12110-24.
    doi: 10.1074/jbc.M809277200pmc: PMC2673280pubmed: 19265192google scholar: lookup
  42. Wang T, Ming Z, Xiaochun W, Hong W. Rab7: role of its protein interaction cascades in endo-lysosomal traffic.. Cell Signal 2011 Mar;23(3):516-21.
    doi: 10.1016/j.cellsig.2010.09.012pubmed: 20851765google scholar: lookup
  43. Chen C, Weisz OA, Stolz DB, Watkins SC, Montelaro RC. Differential effects of actin cytoskeleton dynamics on equine infectious anemia virus particle production.. J Virol 2004 Jan;78(2):882-91.
    doi: 10.1128/JVI.78.2.882-891.2004pmc: PMC368807pubmed: 14694119google scholar: lookup
  44. Chen C, Jin J, Rubin M, Huang L, Sturgeon T, Weixel KM, Stolz DB, Watkins SC, Bamburg JR, Weisz OA, Montelaro RC. Association of gag multimers with filamentous actin during equine infectious anemia virus assembly.. Curr HIV Res 2007 May;5(3):315-23.
    doi: 10.2174/157016207780636542pubmed: 17504173google scholar: lookup
  45. Sandrin V, Sundquist WI. ESCRT requirements for EIAV budding.. Retrovirology 2013 Oct 9;10:104.
    doi: 10.1186/1742-4690-10-104pmc: PMC3907061pubmed: 24107264google scholar: lookup
  46. Tang YD, Na L, Zhu CH, Shen N, Yang F, Fu XQ, Wang YH, Fu LH, Wang JY, Lin YZ, Wang XF, Wang X, Zhou JH, Li CY. Equine viperin restricts equine infectious anemia virus replication by inhibiting the production and/or release of viral Gag, Env, and receptor via distortion of the endoplasmic reticulum.. J Virol 2014 Nov;88(21):12296-310.
    doi: 10.1128/JVI.01379-14pmc: PMC4248950pubmed: 25122784google scholar: lookup
  47. Hinson ER, Cresswell P. The N-terminal amphipathic alpha-helix of viperin mediates localization to the cytosolic face of the endoplasmic reticulum and inhibits protein secretion.. J Biol Chem 2009 Feb 13;284(7):4705-12.
    doi: 10.1074/jbc.M807261200pmc: PMC2640954pubmed: 19074433google scholar: lookup
  48. Inlora J, Chukkapalli V, Derse D, Ono A. Gag localization and virus-like particle release mediated by the matrix domain of human T-lymphotropic virus type 1 Gag are less dependent on phosphatidylinositol-(4,5)-bisphosphate than those mediated by the matrix domain of HIV-1 Gag.. J Virol 2011 Apr;85(8):3802-10.
    doi: 10.1128/JVI.02383-10pmc: PMC3126146pubmed: 21289126google scholar: lookup
  49. Robinson DG, Pimpl P. Clathrin and post-Golgi trafficking: a very complicated issue.. Trends Plant Sci 2014 Mar;19(3):134-9.
    doi: 10.1016/j.tplants.2013.10.008pubmed: 24263003google scholar: lookup

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  1. Mardi N, Haiaty S, Rahbarghazi R, Mobarak H, Milani M, Zarebkohan A, Nouri M. Exosomal transmission of viruses, a two-edged biological sword. Cell Commun Signal 2023 Jan 23;21(1):19.
    doi: 10.1186/s12964-022-01037-5pubmed: 36691072google scholar: lookup
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    doi: 10.1128/jvi.01210-22pubmed: 36448796google scholar: lookup
  3. Bussienne C, Marquet R, Paillart JC, Bernacchi S. Post-Translational Modifications of Retroviral HIV-1 Gag Precursors: An Overview of Their Biological Role. Int J Mol Sci 2021 Mar 11;22(6).
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  5. Vlach J, Eastep GN, Ghanam RH, Watanabe SM, Carter CA, Saad JS. Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly. J Biol Chem 2018 Dec 7;293(49):18828-18840.
    doi: 10.1074/jbc.RA118.003944pubmed: 30309983google scholar: lookup
  6. Watanabe SM, Medina GN, Eastep GN, Ghanam RH, Vlach J, Saad JS, Carter CA. The matrix domain of the Gag protein from avian sarcoma virus contains a PI(4,5)P(2)-binding site that targets Gag to the cell periphery. J Biol Chem 2018 Dec 7;293(49):18841-18853.
    doi: 10.1074/jbc.RA118.003947pubmed: 30309982google scholar: lookup
  7. Ji S, Na L, Ren H, Wang Y, Wang X. Equine Myxovirus Resistance Protein 2 Restricts Lentiviral Replication by Blocking Nuclear Uptake of Capsid Protein. J Virol 2018 Sep 15;92(18).
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