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Methods in molecular biology (Clifton, N.J.)2020; 2123; 361-367; doi: 10.1007/978-1-0716-0346-8_27

Equine Influenza Diagnosis: Sample Collection and Transport.

Abstract: In horses, presumptive diagnosis of equine influenza is commonly made on the basis of clinical signs. This alone is insufficient for confirmation of equine influenza, because other equine infectious respiratory diseases can in some degree have similar clinical presentations. Surveillance and control of equine influenza also necessitate detection of subclinical cases. Effective diagnosis of equine influenza virus infection is critically dependent on obtaining adequate specimens of virus-containing respiratory secretions for testing. These specimens are also valuable as sources for isolation of virus strains for antigenic characterization and potential inclusion in vaccines. Both nasal swabs and nasopharyngeal swabs are employed with horses. These differ little in their invasiveness, but nasopharyngeal swabs typically yield more virus than nasal swabs and are superior diagnostic specimens. Methods for obtaining nasopharyngeal swab specimens are described.
Publication Date: 2020-03-15 PubMed ID: 32170702DOI: 10.1007/978-1-0716-0346-8_27Google Scholar: Lookup
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  • Journal Article

Summary

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The article describes the collection and transport of samples used to diagnose equine influenza, highlighting the importance of obtaining adequate specimens for testing as well as comparing the effectiveness of nasal swabs and nasopharyngeal swabs in the diagnosis of the disease in horses.

The need for Equine Influenza Diagnosis

  • Presumptive diagnosis for equine influenza, a common respiratory illness in horses, is typically done by looking at signs of disease in the animal. However, solely analyzing clinical signs is insufficient for a confirmed diagnosis because other infectious respiratory diseases in horses can manifest with similar symptoms.

Conducting surveillance for Equine Influenza

  • In addition to the confirmation process, surveillance and control measures for equine influenza also require the ability to detect subclinical cases – instances where the animal does not exhibit visible clinical signs.
  • For both these diagnosis and surveillance processes, the collection of virus-containing respiratory specimens from the horse is critical. These samples additionally provide a source for isolating specific strains of the virus for further examination and potential inclusion in preventative vaccines.

Comparison of Nasal Swabs and Nasopharyngeal Swabs

  • Both nasal swabs and nasopharyngeal swabs are commonly used methods of sample collection in horses, with the level of invasiveness being relatively similar.
  • However, the research points out that nasopharyngeal swabs are generally better at yielding more viral samples than nasal swabs, thereby making these swabs superior in producing diagnostic specimens.

Obtaining Nasopharyngeal Swabs

  • The article describes in detail the process of obtaining nasopharyngeal swab specimens. Despite not providing information on the method itself, it emphasizes the importance of this method considering its superiority in yielding diagnostic specimens.

Cite This Article

APA
Chambers TM, Reedy SE. (2020). Equine Influenza Diagnosis: Sample Collection and Transport. Methods Mol Biol, 2123, 361-367. https://doi.org/10.1007/978-1-0716-0346-8_27

Publication

ISSN: 1940-6029
NlmUniqueID: 9214969
Country: United States
Language: English
Volume: 2123
Pages: 361-367

Researcher Affiliations

Chambers, Thomas M
  • Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. tmcham1@uky.edu.
Reedy, Stephanie E
  • Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.

MeSH Terms

  • Animals
  • Horse Diseases / diagnosis
  • Horse Diseases / virology
  • Horses / virology
  • Nasopharynx / virology
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / veterinary
  • Orthomyxoviridae Infections / virology
  • Specimen Handling / methods
  • Transportation

Citations

This article has been cited 1 times.
  1. Pusterla N, Lawton K, Barnum S, Magdesian KG. Comparison of Nose Wipes, Stall Sponges, and Air Samples with Nasal Secretions for the Molecular Detection of Equine Influenza Virus in Clinically and Subclinically Infected Horses. Viruses 2025 Mar 20;17(3).
    doi: 10.3390/v17030449pubmed: 40143375google scholar: lookup