Equine ocular mast cell tumor: histopathological and immunohistochemical description.
Abstract: This report describes an ocular mast cell tumor in a 13-year-old female sport horse. Clinical examination revealed a solitary firm mass located in the ocular mucosa, protruding from behind the left lower eyelid. The lesion was surgically removed and submitted to histopathology. Microscopically, the mass was composed of sheets of well-differentiated neoplastic round cells circumscribed by delicate connective tissue. Positive Giemsa and Toluidine Blue staining confirmed the presence of cytoplasmic granules. Neoplastic cells showed strong membranous and mild diffuse cytoplasmic immunoreactivity for c-KIT and a low KI-67 proliferative index. Based on these findings, a diagnosis of ocular mast cell tumor was made. Six months after surgical removal, no evidence of ocular lesion recurrence was detected.
Publication Date: 2017-12-16 PubMed ID: 29270072PubMed Central: PMC5735312DOI: 10.1294/jes.28.149Google Scholar: Lookup
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Summary
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The research paper reports a case of ocular mast cell tumor in a 13-year-old female sport horse, its associated clinical examination, histopathological and immunohistochemical findings and outcome of surgical removal of the tumor.
Introduction and Case Examination
- This study features a case where a female sport horse developed an ocular mast cell tumor. The primary indication of this was a solitary firm mass noticeable on the ocular mucosa, specifically behind the left lower eyelid.
- The mass was then surgically removed presumably because of the likely discomfort or potential harm it could bring to the horse. The removed tumor was subjected to further detailed evaluation and analysis to identify its histopathology and immunohistochemistry.
Test Results and Diagnosis
- Conducting a microscopic examination of the mass revealed that the tumor was made up of sheets of well-differentiated neoplastic round cells. These cells were surrounded by intricate connective tissues.
- The presence of cytoplasmic granules in the cells was validated through positive Giemsa and Toluidine Blue staining. This stained granules represent an essential characteristics of mast cells.
- Moreover, neoplastic cells showed strong membranous immunoreactivity for c-KIT, which is a recognized marker for mast cells. Mild diffuse cytoplasmic immunoreactivity was also observed.
- The cells showed a low KI-67 proliferative index, which suggests a lower growth speed of the tumor cells. This may explain why this was possibly not a malignant or aggressive tumor.
- Based on these findings, a diagnosis of ocular mast cell tumor was confirmed.
Outcome after Surgical Removal
- A follow-up examination done six months after the surgical removal of the tumor revealed no evidence of the recurrence of the lesion. This suggests that the surgical removal was successful and the horse remained free of such ocular lesion.
Cite This Article
APA
Flores AR, Azinhaga A, Pais E, Faria F, Nunes F, Gartner F, Amorim I.
(2017).
Equine ocular mast cell tumor: histopathological and immunohistochemical description.
J Equine Sci, 28(4), 149-152.
https://doi.org/10.1294/jes.28.149 Publication
Researcher Affiliations
- Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
- Vetplus Veterinary Clinic Lda, 3200-532 Tomar, Portugal.
- Vetplus Veterinary Clinic Lda, 3200-532 Tomar, Portugal.
- Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
- Vasco da Gama University School (EUVG), 3020-210 Coimbra, Portugal.
- Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
- Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal.
- Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), 4200-465 Porto, Portugal.
- Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
- Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal.
- Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), 4200-465 Porto, Portugal.
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