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Home / Equine Research Bank / Vaccines (Basel) / Equine PBMC Cytokines Profile after In Vitro α- and &#...
Vaccines2017; 5(3); 28; doi: 10.3390/vaccines5030028

Equine PBMC Cytokines Profile after In Vitro α- and γ-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment.

Abstract: Equine herpesviruses (EHV) infect horses early during life and the persistence of these viruses through establishment of latency represents a real risk. A better understanding of the immune response to EHV infection is necessary to improve our methods of prevention and decrease the risk of transmission. The objectives of this study were to characterise the cytokine gene expression profile of peripheral blood mononuclear cells (PBMC) after in vitro EHV-1, EHV-4, and EHV-2 infection and to determine the efficacy of inactivated (iPPVO) against these 3 viruses. PBMC were isolated from 3 horses and infected in vitro with EHV-1, EHV-4, or EHV-2 in the presence or absence of iPPVO. In vitro culture of PBMC with EHV-1, EHV-4, and iPPVO induced a significant increase of IFN-α, IFN-β, and IFN-γ gene expression. EHV-4 also triggered a significant increase of IL-6 and TNF-α mRNA. EHV-2 triggered a significant increase of IFN-α, IFN-β, IFN-γ, IL-1β, IL-6, and TNF-α mRNA. The presence of iPPVO induced an earlier and stronger expression of IFN-α, IFN-β, and IFN-γ mRNA during EHV infection and reduced the inflammatory response induced by EHV-2. In conclusion, this study suggests that the presence of iPPVO potentiates the development of the immune response to in vitro EHV infection.
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Publication Date: 2017-09-19 PubMed ID: 28925977PubMed Central: PMC5620559DOI: 10.3390/vaccines5030028Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the effect of equine herpesviruses (EHV) on the cytokine gene expression in horses’ peripheral blood mononuclear cells (PBMC) and evaluates the effectiveness of an immunomodulator in countering these viruses.

Background of the Study

  • This research looks into equine herpesviruses (EHV). The EHV infect horses early in their lives and have a latent effect which could pose risks in the long term.
  • The study aims to dive into the prevention methods and reduce transmission risks by understanding the immune response to EHV infection.

Objectives of the Study

  • The research’s most crucial goals are to understand the cytokine gene expression profile in horses’ peripheral blood mononuclear cells (PBMC) after EHV-1, EHV-4, and EHV-2 in vitro infection.
  • It also seeks to assess the efficacy of an immunomodulator, inactivated Parapoxvirus Ovis (iPPVO) in countering these viruses.

Study’s Methodology

  • The researchers isolated PBMC from three horses and infected them in vitro with EHV-1, EHV-4, or EHV-2. They conducted these tests either in the presence or absence of the iPPVO.

Results of the Study

  • The results showed that culturing PBMC with EHV-1, EHV-4, and iPPVO induced a significant increase in the expression of IFN-α, IFN-β, and IFN-γ genes.
  • EHV-4 triggered a significant escalation of IL-6 and TNF-α mRNA, while EHV-2 caused a notable increase in IFN-α, IFN-β, IFN-γ, IL-1β, IL-6, and TNF-α mRNA.
  • The presence of iPPVO led to an early and more vigorous IFN-α, IFN-β, and IFN-γ mRNA expression during EHV infection, curbing the inflammatory response induced by EHV-2.

Conclusion of the Study

  • The study concludes that the presence of the immunomodulator iPPVO strengthens the development of the immune response to in vitro EHV infection.

Cite This Article

APA
Hue ES, Richard EA, Fortier CI, Fortier GD, Paillot R, Raue R, Pronost SL. (2017). Equine PBMC Cytokines Profile after In Vitro α- and γ-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment. Vaccines (Basel), 5(3), 28. https://doi.org/10.3390/vaccines5030028

Publication

Vaccines
ISSN: 2076-393X
NlmUniqueID: 101629355
Country: Switzerland
Language: English
Volume: 5
Issue: 3
PII: 28

Researcher Affiliations

Hue, Erika S
  • LABÉO Frank Duncombe, Unité BioTARGen, EA 7450, Normandie Université, 14053 Caen, France. erika.hue@laboratoire-labeo.fr.
Richard, Eric A
  • LABÉO Frank Duncombe, Unité BioTARGen, EA 7450, Normandie Université, 14053 Caen, France. eric.richard@laboratoire-labeo.fr.
Fortier, Christine I
  • LABÉO Frank Duncombe, Unité BioTARGen, EA 7450, Normandie Université, 14053 Caen, France. christine.fortier@laboratoire-labeo.fr.
Fortier, Guillaume D
  • LABÉO Frank Duncombe, Unité BioTARGen, EA 7450, Normandie Université, 14053 Caen, France. guillaume.fortier@laboratoire-labeo.fr.
Paillot, Romain
  • Animal Health Trust, Centre for Preventive Medicine, Lanwades Park, Newmarket CB8 7UU, UK. romain.paillot@aht.org.uk.
Raue, Rudiger
  • Veterinary Medicine Research and Development, Zoetis Belgium, 1930 Zaventem, Belgium. rudiger.raue@zoetis.com.
Pronost, Stéphane L
  • LABÉO Frank Duncombe, Unité BioTARGen, EA 7450, Normandie Université, 14053 Caen, France. stephane.pronost@laboratoire-labeo.fr.

Conflict of Interest Statement

iPPVO is marketed as Zylexis and was provided by Zoetis. This project received some financial support from Zoetis. Rudiger Raue is employed by Zoetis. Stephane Pronost conducted a remunerated review for Zoetis and received honoraria for conference presentations. The other authors declare no conflict of interest.

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Citations

This article has been cited 6 times.
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