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Home / Equine Research Bank / J Vet Diagn Invest / Equine renal hemangiosarcoma: clinical presentation, patholo...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2017; 30(2); 268-274; doi: 10.1177/1040638717745575

Equine renal hemangiosarcoma: clinical presentation, pathologic features, and pSTAT3 expression.

Abstract: Hemangiosarcoma is an uncommon tumor in horses. We characterized 3 cases of equine renal hemangiosarcoma, focusing on clinical and pathologic features, and describe occurrence of the epithelioid variant of hemangiosarcoma in one of these cases. Nuclear expression of phosphorylated STAT3 (pSTAT3) was assessed to analyze potential inappropriate STAT3 activation as a component of tumor pathogenesis. Clinical signs in the 3 horses included insidious weight loss, followed in one case by serosanguineous nasal discharge and terminal epistaxis, and nonspecific signs of abdominal pain. Two of the hemangiosarcomas had a classical histopathologic appearance; in the other, neoplastic cells were polygonal and were arranged in densely packed sheets, resembling the epithelioid variant. Cross-reactivity of a pSTAT3 antibody was established by demonstration of pSTAT3 expression in the epithelium of glabrous skin by immunoblotting and immunohistochemistry. In the epithelioid hemangiosarcoma, ~40% of neoplastic cells exhibited nuclear pSTAT3 expression, but in the other 2 cases, expression was weak and variable in the neoplastic population, although stromal cell pSTAT3 activity was evident in pulmonary metastases in one case.
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Publication Date: 2017-12-03 PubMed ID: 29199537PubMed Central: PMC6505882DOI: 10.1177/1040638717745575Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates three instances of a rare tumor in horses known as equine renal hemangiosarcoma, focusing on their clinical and pathological qualities, and the potential role of STAT3 activation in the development of the tumor.

Study Overview

  • The study involved characterizing three cases of equine renal hemangiosarcoma, which is a rare vascular tumor in horses.
  • The aim of the investigation was to gain an understanding of the clinical and pathologic features of the tumor, including the first reported case of the epithelioid variant, a type of hemangiosarcoma.
  • Additionally, the researchers wanted to assess the role of phosphorylated STAT3 (pSTAT3), a protein associated with cell growth and proliferation, in the development of these tumors.

Clinical Presentation

  • The clinical signs in the horses with these tumors manifested as subtle weight loss, with one also experiencing serosanguineous nasal discharge, sudden nosebleed, and non-specific discomfort in the abdomen.

Pathologic Features and pSTAT3 Expression

  • Two of the hemangiosarcomas presented with classical histopathologic appearance whereas the third case had cells arranged in a densely packed sheet, which is characteristic of the epithelioid variant.
  • Antibodies to pSTAT3 were used to assess the expression of pSTAT3 in the tumors. The researchers validated the antibodies by showing that they can bind to pSTAT3 in the skin cells in an experiment with immunoblotting and immunohistochemistry techniques.
  • In the equine with epithelioid hemangiosarcoma, approximately 40% of tumor cells had nuclear pSTAT3 expression, indicating increased STAT3 activity.
  • However, the two other cases had weaker and variable pSTAT3 expression within the neoplastic cells. Despite this, signs of pSTAT3 activity persisted in the stroma cells of pulmonary metastases in one equine.

Implications

  • The findings provide valuable insight into the clinical and pathologic features of equine renal hemangiosarcoma. This information can contribute to the identification and management of this rare horse tumor.
  • The study also suggests a potential role of pSTAT3 in the pathogenesis of equine renal hemangiosarcoma, warranting further explorative research on this signaling protein and its potential as a therapeutic target.

Cite This Article

APA
Hughes K, Scott VHL, Blanck M, Barnett TP, Spanner Kristiansen J, Foote AK. (2017). Equine renal hemangiosarcoma: clinical presentation, pathologic features, and pSTAT3 expression. J Vet Diagn Invest, 30(2), 268-274. https://doi.org/10.1177/1040638717745575

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 30
Issue: 2
Pages: 268-274

Researcher Affiliations

Hughes, Katherine
    Scott, Victoria H L
      Blanck, Maximilian
      • 2 Horizon Discovery, Waterbeach, Cambridge, UK.
      Barnett, Timothy P
        Spanner Kristiansen, Jannie
          Foote, Alastair K

            MeSH Terms

            • Animals
            • Biomarkers, Tumor / metabolism
            • Cell Nucleus / pathology
            • Diagnosis, Differential
            • Female
            • Hemangiosarcoma / diagnosis
            • Hemangiosarcoma / metabolism
            • Hemangiosarcoma / veterinary
            • Horse Diseases / diagnosis
            • Horse Diseases / metabolism
            • Horse Diseases / pathology
            • Horses
            • Immunohistochemistry / veterinary
            • Kidney Neoplasms / diagnosis
            • Kidney Neoplasms / metabolism
            • Kidney Neoplasms / veterinary
            • Male
            • STAT3 Transcription Factor / metabolism

            Conflict of Interest Statement

            The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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            Citations

            This article has been cited 1 times.
            1. Shih CH, Chang YC, Lai YC, Chiou HY. Investigating the role of signal transducer and activator of transcription 3 in feline injection site sarcoma. BMC Vet Res 2022 Jul 14;18(1):276.
              doi: 10.1186/s12917-022-03352-ypubmed: 35836213google scholar: lookup
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