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Animals : an open access journal from MDPI2024; 14(16); 2299; doi: 10.3390/ani14162299

Equine Sarcocystosis in the Northern Region of the Republic of Kazakhstan.

Abstract: A total of 396 samples were taken from the hearts, oesophagi, and diaphragms of 132 horses slaughtered at slaughterhouses in 2023 for subsequent examination. Methods: The histological method revealed pathomorphological changes in the muscle tissue. The molecular method identified the pathogen species. Results: Histological examination revealed thick-walled cysts with internal septa and numerous bradyzoites, and mononuclear inflammatory cells with pericyst infiltrates. Microcyst samples were amplified by polymerase chain reaction. Molecular genetic analysis allowed for the identification of 18 sarcocysts. Phylogenetic analysis of isolates revealed three separate clades of and two separate clades of . Comparison and phylogenetic analysis revealed a very close relationship between the identified species and other equine DNA sequences from China and Japan. Based on the results obtained, the epizootic situation and the parasitic level of sarcocystosis contamination of horses in the northern Kazakhstan were determined. Conclusions: This is the first histological and molecular study to identify spp. isolated from microscopic forms of equine sarcocysts in the northern Kazakhstan. This research will contribute to the fight against the spread of sarcocystosis in the Republic of Kazakhstan and will allow us to develop proposals for improving the mechanisms of sarcocystosis control.
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Publication Date: 2024-08-07 PubMed ID: 39199833PubMed Central: PMC11350656DOI: 10.3390/ani14162299Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the presence and type of sarcocystosis, a type of parasite, in horses in the northern region of Kazakhstan. The study, which examined tissue samples from while also conducting molecular analysis, identified 18 instances of sarcocysts and for the first time identified microscopic forms of equine sarcocystosis in this geographic region.

Methodology

  • The research involved examination of 396 samples taken from the hearts, esophagi, and diaphragms of 132 horses that were slaughtered at slaughterhouses in Kazakhstan in 2023.
  • The method utilized both a histological method and molecular method to conduct the research.
  • The histological method involved a close examination of the muscle tissue to identify signs of pathomorphological changes.
  • The molecular method used was to identify the species causing the disease.

Results

  • In the muscle tissue, histological examination unveiled thick-walled cysts with internal septa and numerous bradyzoites that are suggestive of sarcocystosis infection.
  • They also detected the presence of mononuclear inflammatory cells, which had infiltrated areas around the cysts.
  • The researchers used polymerase chain reaction to amplify samples of the microcysts.
  • From the molecular genetic examination, they were able to identify 18 sarcocysts.
  • The researchers undertook a phylogenetic analysis on the isolates and discovered three separate clades of Sarcocystis falcatula and two separate clades of Sarcocystis neurona.
  • The genetic relationship of these identified species with other equine Sarcocystis species in China and Japan was found to be similar when compared.

Conclusions

  • This research marked a significant first step in the histological and molecular identification of Sarcocystis species in microscopic forms of equine sarcocysts in the northern region of Kazakhstan.
  • The findings helped to establish the epizootic situation and the parasitic load of sarcocystosis infection in horses of the Northern Kazakhstan region.
  • This kind of research can aid in the implementation of improved sarcocystosis control methods to prevent its spread in the Republic of Kazakhstan and potentially in other locations.

Cite This Article

APA
Bermukhametov Z, Suleimanova K, Tomaruk O, Baimenov B, Shevchenko P, Batyrbekov A, Mikniene Z, Onur Girişgin A, Rychshanova R. (2024). Equine Sarcocystosis in the Northern Region of the Republic of Kazakhstan. Animals (Basel), 14(16), 2299. https://doi.org/10.3390/ani14162299

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 16
PII: 2299

Researcher Affiliations

Bermukhametov, Zhanaidar
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.
Suleimanova, Kulyay
  • Department of Natural Sciences, Kostanay Social-Technical University named after Academician Zulharnai Aldamjar, Kostanay 110000, Kazakhstan.
Tomaruk, Oksana
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.
Baimenov, Bakhit
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.
Shevchenko, Pavel
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.
Batyrbekov, Assylbek
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.
Mikniene, Zoja
  • Large Animal Clinic, Lithuanian University of Health Science, Veterinary Academy, LT-44307 Kaunas, Lithuania.
Onur Girişgin, Ahmet
  • Department of Parasitology, Veterinary Faculty, Bursa Uludag University, 16059 Nilüfer-Bursa, Turkey.
Rychshanova, Raushan
  • Research and Innovation Center, Research Institute of Applied Biotechnology, NLC «Akhmet Baitursynuly Kostanay Regional University», Kostanay 110000, Kazakhstan.

Grant Funding

  • Grant No. AP14869992 / Ministry of Education and Science of the Republic of Kazakhstan

Conflict of Interest Statement

The authors declare no conflicts of interest.

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Citations

This article has been cited 1 times.
  1. Ullah A, Geng M, Chen W, Zhu Q, Shi L, Zhang X, Akhtar MF, Wang C, Khan MZ. Effect of Parasitic Infections on Hematological Profile, Reproductive and Productive Performance in Equines. Animals (Basel) 2025 Nov 14;15(22).
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