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Home / Equine Research Bank / Vet J / Equine sarcoid: In situ demonstration of matrix metalloprote...
Veterinary journal (London, England : 1997)2014; 202(2); 279-285; doi: 10.1016/j.tvjl.2014.07.026

Equine sarcoid: In situ demonstration of matrix metalloproteinase expression.

Abstract: Sarcoids are the most prevalent equine skin tumours and remain a therapeutic challenge due to their differing clinical morphology, local aggressive behaviour, and high recurrence following surgical treatment. In vitro, sarcoid derived fibroblasts are invasive and express matrix metalloproteinase (MMP) -1, -2 and -9. It was hypothesised that the MMPs produced by neoplastic cells play a role in both their local invasiveness and interaction with the overlying epidermis (picket fence formation). The objective of this morphological study was to investigate the local behaviour and in situ MMP expression pattern in sarcoids of different clinical types. A total of 43 surgically excised sarcoids were examined by histology, immunohistology for the expression of MMP-1, -2 and -9, and transmission electron microscopy. Regardless of the clinical type, sarcoids showed local invasion of the dermis and damage to the basement membrane in areas of interaction with the epidermis. This was associated with MMP-1 expression in both neoplastic cells and epidermis. The results suggest a link between MMP-1 expression and the local aggressiveness of sarcoids regardless of the clinical type.
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Publication Date: 2014-08-28 PubMed ID: 25439440PubMed Central: PMC7128672DOI: 10.1016/j.tvjl.2014.07.026Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examines the role of certain proteins (MMP -1, -2 and -9) in the aggressive behavior of equine sarcoids, a prevalent skin tumor in horses, and how they may contribute to their high recurrence rate after surgical treatment.

Study Goals and Methods

  • The goal of the study was to understand the local behavior and expression patterns of matrix metalloproteinase (MMP), specific proteins known to be associated with the tissue remodeling of cancerous cells, in various clinical types of sarcoids. The researchers hypothesize that these proteins are partly responsible for the tumors’ aggressive behavior and high risk of recurrence after surgery.
  • To gather data, a total of 43 surgically removed sarcoids were examined using three different techniques: histology, immunohistology, and transmission electron microscopy. Histology allowed researchers to study the structure of the tumors, while immunohistology was used to inspect the expression of MMP-1, -2, and -9 and electron microscopy gave detailed images at a cellular level.

Findings

  • The researchers found that, no matter the clinical type of sarcoid, the tumors demonstrated behavior of local invasion into the dermis, or the second layer of skin, and damages to the basement membrane, a crucial boundary between the outermost layer of skin and the underlying tissue.
  • Moreover, these invasive behaviors were found to be associated with the expression of MMP-1, both in the tumor cells and the epidermis, the outermost layer of skin. This connection suggests that MMP-1 may play a crucial role in the local aggressive behavior and high recurrence of equine sarcoids.

Conclusions

  • This study brings us one step closer to understanding the pathophysiology of equine sarcoids, the most common skin tumor in horses. The strong link between the tumors’ aggressive behaviors and the expression of MMP-1, not only in the tumor cells but also in the epidermis, suggests the significant role this protein plays in the local invasion and high recurrence of the disease.
  • This insight could lead to targeted therapeutic approaches in managing equine sarcoids, potentially reducing the likelihood of recurrence and improving outcomes following surgeries. Further research into MMP-1’s role could also provide insights into other similar skin tumors in both animals and humans.

Cite This Article

APA
Mosseri S, Hetzel U, Hahn S, Michaloupoulou E, Sallabank HC, Knottenbelt DC, Kipar A. (2014). Equine sarcoid: In situ demonstration of matrix metalloproteinase expression. Vet J, 202(2), 279-285. https://doi.org/10.1016/j.tvjl.2014.07.026

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 202
Issue: 2
Pages: 279-285
PII: S1090-0233(14)00318-9

Researcher Affiliations

Mosseri, S
  • Philip Leverhulme Equine Hospital, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Wirral CH64 7TE, UK; Three Counties Equine Hospital, Ripple, Tewkesbury GL20 6HE, UK.
Hetzel, U
  • Veterinary Pathology, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00014 Helsinki, Finland.
Hahn, Shelley
  • Veterinary Pathology, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00014 Helsinki, Finland.
Michaloupoulou, Eleni
  • Veterinary Epidemiology and Public Health, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Wirral CH64 7TE, UK.
Sallabank, Hannah Clare
  • Veterinary Pathology, School of Veterinary Science, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK; Department of Infection Biology, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK.
Knottenbelt, Derek C
  • Philip Leverhulme Equine Hospital, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Wirral CH64 7TE, UK.
Kipar, A
  • Veterinary Pathology, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00014 Helsinki, Finland; Veterinary Pathology, School of Veterinary Science, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK; Department of Infection Biology, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK. Electronic address: anja.kipar@uzh.ch.

MeSH Terms

  • Animals
  • Female
  • Gene Expression Regulation, Neoplastic
  • Histology
  • Horse Diseases / genetics
  • Horses
  • Male
  • Matrix Metalloproteinase 1 / genetics
  • Matrix Metalloproteinase 1 / metabolism
  • Matrix Metalloproteinase 2 / genetics
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Microscopy, Electron, Transmission / veterinary
  • Sarcoidosis / genetics
  • Sarcoidosis / veterinary
  • Skin Neoplasms / genetics
  • Skin Neoplasms / veterinary

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Citations

This article has been cited 6 times.
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  2. Daraban Bocaneti F, Altamura G, Corteggio A, Tanase OI, Dascalu MA, Pasca SA, Hritcu O, Mares M, Borzacchiello G. Expression of matrix metalloproteinases (MMPs)-2/-7/-9/-14 and tissue inhibitors of MMPs (TIMPs)-1/-2 in bovine cutaneous fibropapillomas associated with BPV-2 infection.. Front Vet Sci 2022;9:1063580.
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