Equine suspensory ligament and tendon explants cultured with platelet-rich gel supernatants release different anti-inflammatory and anabolic mediators.
Abstract: The aim of this study was to evaluate the release of pro- and anti-inflammatory as well as anabolic mediators stimulated by a leukocyte-reduced platelet-rich gel supernatant (Lr-PRGS) and a leukocyte-reduced plasma supernatant (Lr-PL) at two concentrations (25 and 50%) on normal equine suspensory ligament explants (SLEs) and tendon explants (TEs). SLEs and TEs from six horses were independently incubated for 48 h with Lr-PRGS and Lr-PL at concentrations of 25 and 50%, respectively. Samples were collected from the incubated tissues at 1 h and 48 h, which were employed for ELISA determination of interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), IL-4, IL-1 receptor antagonist (IL-1ra), platelet-derived growth factor isoform BB (PDGF-BB), transforming growth factor beta-1 (TGF-β, and hyaluronic acid (HA). Overall, 50% Lr-PRGS induced significantly less IL-1β release than the other hemoderivatives in both tissues. At 48 h, both Lr-PRGS and 25% Lr-PL induced significantly higher TNF-α concentrations in SLEs when compared to TEs, whereas both Lr-PRGS concentrations induced significantly higher IL-4 concentrations in SLEs in comparison to TEs. IL-1ra release was not different between tissues. However, this cytokine was significantly higher in tissue explants cultured with both Lr-PRGS concentrations. HA concentration was lower in tissue explants cultured with all hemoderivatives at two concentrations when compared to the control group. The positive effects observed for ligaments and tendons treated with Lr-PRGS may be mediated by the inhibition of IL-1β release of and increased release of IL-4 and IL-1ra. Furthermore, PDGF-BB could be a polypeptide responsible for mediating the release of anti-inflammatory cytokines in SLEs and TEs incubated with Lr-PRGS.
Copyright © 2018 Elsevier Masson SAS. All rights reserved.
Publication Date: 2018-09-19 PubMed ID: 30241051DOI: 10.1016/j.biopha.2018.09.065Google Scholar: Lookup
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Summary
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This research paper investigates how certain supernatants influence the release of different anti-inflammatory and anabolic mediators in horse suspensory ligament and tendon explants. It was observed that leukocyte-reduced platelet-rich gel supernatants (Lr-PRGS) can effectively reduce certain inflammatory cytokines while promoting the release of certain others, potentially providing therapeutic benefits.
Experimental Setup
- The subjects of the study were suspensory ligament explants (SLEs) and tendon explants (TEs) from six horses.
- The explants were independently incubated for 48 hours with two types of supernatants, leukocyte-reduced platelet-rich-gel supernatant (Lr-PRGS) and leukocyte-reduced plasma supernatant (Lr-PL), and at two different concentrations (25% and 50%).
Methodology
- Samples were collected from the incubated tissues at the 1-hour and 48-hour marks for testing.
- An Elisa determination was conducted on interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), IL-1 receptor antagonist (IL-1ra), platelet-derived growth factor isoform BB (PDGF-BB), transforming growth factor beta-1(TGF-β, and hyaluronic acid (HA).
Findings
- Lr-PRGS at 50% concentration induced significantly less IL-1β release than other hemoderivatives in both tissues.
- Both Lr-PRGS and 25% Lr-PL at 48 hours induced higher TNF-α concentrations in SLEs compared to TEs.
- Both concentrations of Lr-PRGS induced significantly higher IL-4 concentrations in SLEs compared to TEs.
- There was no significant difference in IL-1ra release between tissues. However, this cytokine’s release was significantly higher in tissue explants cultured with both concentrations of Lr-PRGS.
- HA concentration was lower in tissue explants cultured with all hemoderivatives at both concentrations compared to the control group.
Conclusions
- The positive impacts observed for ligaments and tendons treated with Lr-PRGS could be due to the inhibition of IL-1β release and increased release of IL-4 and IL-1ra.
- Furthermore, PDGF-BB could potentially mediate the release of anti-inflammatory cytokines in SLEs and TEs incubated with Lr-PRGS.
Cite This Article
APA
Bonilla-Gutiérrez AF, Castillo-Franz C, López C, Álvarez ME, Giraldo CE, Carmona JU.
(2018).
Equine suspensory ligament and tendon explants cultured with platelet-rich gel supernatants release different anti-inflammatory and anabolic mediators.
Biomed Pharmacother, 108, 476-485.
https://doi.org/10.1016/j.biopha.2018.09.065 Publication
Researcher Affiliations
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. Electronic address: afbonillag@gmail.com.
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia; Grupo de Investigación en Medicina Veterinaria GIVET, Corporación Universitaria Lasallista, Caldas, Colombia; Programa de Doctorado en Ciencias Veterinarias, Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Becario Doctorado Nacional CONICYT, Valdivia, Chile. Electronic address: cricastillo@lasallistadocentes.edu.co.
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. Electronic address: catalina.lopez@ucaldas.edu.co.
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. Electronic address: maria.alvarez_l@ucaldas.edu.c.
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. Electronic address: cgiraldo@ucaldas.edu.co.
- Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia. Electronic address: carmona@ucaldas.edu.co.
MeSH Terms
- Animals
- Anti-Inflammatory Agents / metabolism
- Blood Platelets / metabolism
- Gels / metabolism
- Horses
- Hyaluronic Acid / metabolism
- Interleukin 1 Receptor Antagonist Protein / metabolism
- Interleukin-1beta / metabolism
- Interleukin-4 / metabolism
- Leukocytes / metabolism
- Ligaments / metabolism
- Tendons / metabolism
- Transforming Growth Factor beta1 / metabolism
Citations
This article has been cited 4 times.- Lu J, Li H, Zhang Z, Xu R, Wang J, Jin H. Platelet-rich plasma in the pathologic processes of tendinopathy: a review of basic science studies.. Front Bioeng Biotechnol 2023;11:1187974.
- Carmona JU, López C, Ceballos-Márquez A. Temporal Release and Denature of Several Mediators in Pure Platelet-Rich Plasma and Temperature-Induced Platelet Lysates Derived from a Similar Bovine Platelet Concentrate.. Vet Med Int 2022;2022:2609508.
- Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade.. Front Vet Sci 2021;8:641818.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
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