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Veterinary immunology and immunopathology1993; 35(3-4); 225-238; doi: 10.1016/0165-2427(93)90036-4

Equine T-lymphocyte MHC II expression: variation with age and subset.

Abstract: This paper describes the characteristics of a monoclonal antibody (CVS10) that reacts with an equine leukocyte antigen. On the basis of tissue distribution and biochemical characteristics, this antigen is equine MHC II. The equine MHC II antigen was found on a large subset of T-lymphocytes in addition to all B-lymphocytes, as has been reported previously. In addition MHC II was found to be present on a large proportion of both the mutually exclusive equine T-lymphocyte subpopulations which express either the equine homologues of CD4, or CD8. In a study of changes in equine MHC II expression with age it was found that far fewer lymphocytes express MHC II in neonatal foals than in adult horses. An increase with age was demonstrated. This age related change in MHC II expression was shown to occur principally within the T-lymphocyte subpopulation. It is proposed that this change may be associated with the development of a memory T-lymphocyte population, and that MHC II expression may be a marker for memory T-lymphocytes.
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Publication Date: 1993-01-01 PubMed ID: 8094263DOI: 10.1016/0165-2427(93)90036-4Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper presents a study on the behavior of a particular equine leukocyte antigen, equine MHC II. The authors note changes in the expression of this antigen with age, and further analyze its presence within specific T-lymphocyte subpopulations. Their findings propose a link between this MHC II expression and the development of memory T-lymphocytes within horses.

Objectives and Methodology

  • The main objective of this study was to understand the characteristics and behavior of the monoclonal antibody CVS10, which reacts with an equine leukocyte antigen identified as equine MHC II.
  • Furthermore, the study aimed to track how the expression of this antigen varies with age and different T-lymphocyte subsets.
  • The researchers used neonatal foals and adult horses for their study. They performed analyses to identify the equine MHC II antigen on various lymphocyte subsets in their subjects.

Findings on MHC II Antigen Presence

  • In agreement with previous reports, the equine MHC II antigen was found to be present on all B-lymphocytes.
  • Besides B-lymphocytes, this antigen was also found on a significant subset of T-lymphocytes.
  • Eleminanting the possibilities of mutual exclusivity, this antigen was identified on a large portion of equine T-lymphocyte subpopulations expressing either the homologues of CD4 or CD8.

Observations on Changes with Age

  • The researchers noted a significant change in equine MHC II expression when comparing age groups. In neonatal foals, far fewer lymphocytes were found to express MHC II as compared to adult horses.
  • This trend suggested an increase in MHC II expression with age.
  • The age-related change in MHC II expression was most distinct within the T-lymphocyte subpopulation.

Conclusion and Proposal

  • The results from the study led researchers to propose that the observed age-related changes in MHC II expression could be associated with the development of a memory T-lymphocyte population.
  • They suggested that MHC II expression may serve as a marker for these memory T-lymphocytes, helping track their development and understanding their role in the immune response.

Cite This Article

APA
Lunn DP, Holmes MA, Duffus WP. (1993). Equine T-lymphocyte MHC II expression: variation with age and subset. Vet Immunol Immunopathol, 35(3-4), 225-238. https://doi.org/10.1016/0165-2427(93)90036-4

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 35
Issue: 3-4
Pages: 225-238

Researcher Affiliations

Lunn, D P
  • Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Holmes, M A
    Duffus, W P

      MeSH Terms

      • Aging / immunology
      • Animals
      • Antibodies, Monoclonal / biosynthesis
      • Antibodies, Monoclonal / immunology
      • CD4-Positive T-Lymphocytes / immunology
      • Electrophoresis, Polyacrylamide Gel
      • Flow Cytometry
      • Fluorescent Antibody Technique
      • Histocompatibility Antigens Class II / immunology
      • Horses / immunology
      • Mice
      • Mice, Inbred BALB C
      • T-Lymphocytes / immunology
      • T-Lymphocytes, Regulatory / immunology

      Citations

      This article has been cited 8 times.
      1. Krakowski L, Bartoszek P, Krakowska I, Stachurska A, Piech T, Brodzki P, Wrona Z. Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition. J Vet Res 2017 Jun;61(2):217-221.
        doi: 10.1515/jvetres-2017-0028pubmed: 29978076google scholar: lookup
      2. Cavatorta DJ, Erb HN, Felippe MJ. Activation-induced FoxP3 expression regulates cytokine production in conventional T cells stimulated with autologous dendritic cells. Clin Vaccine Immunol 2012 Oct;19(10):1583-92.
        doi: 10.1128/CVI.00308-12pubmed: 22855393google scholar: lookup
      3. Tallmadge RL, Stokol T, Gould-Earley MJ, Earley E, Secor EJ, Matychak MB, Felippe MJ. Fell Pony syndrome: characterization of developmental hematopoiesis failure and associated gene expression profiles. Clin Vaccine Immunol 2012 Jul;19(7):1054-64.
        doi: 10.1128/CVI.00237-12pubmed: 22593239google scholar: lookup
      4. Fidalgo-Carvalho I, Craigo JK, Barnes S, Costa-Ramos C, Montelaro RC. Characterization of an equine macrophage cell line: application to studies of EIAV infection. Vet Microbiol 2009 Apr 14;136(1-2):8-19.
        doi: 10.1016/j.vetmic.2008.10.010pubmed: 19038510google scholar: lookup
      5. Davis WC, Hamilton MJ. Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules. J Vet Sci 2008 Mar;9(1):51-66.
        doi: 10.4142/jvs.2008.9.1.51pubmed: 18296889google scholar: lookup
      6. Flaminio MJ, Borges AS, Nydam DV, Horohov DW, Hecker R, Matychak MB. The effect of CpG-ODN on antigen presenting cells of the foal. J Immune Based Ther Vaccines 2007 Jan 25;5:1.
        doi: 10.1186/1476-8518-5-1pubmed: 17254326google scholar: lookup
      7. Lunn DP, McClure JT, Schobert CS, Holmes MA. Abnormal patterns of equine leucocyte differentiation antigen expression in severe combined immunodeficiency foals suggests the phenotype of normal equine natural killer cells. Immunology 1995 Mar;84(3):495-9.
        pubmed: 7751035
      8. Chitta S, Nehete BP, Delise AB, Simmons JH, Nehete PN. Reactivity of HLADR antibody manifests expression of surface MHC II molecules on peripheral blood T lymphocytes in new world monkeys. Immun Inflamm Dis 2024 Jun;12(6):e1318.
        doi: 10.1002/iid3.1318pubmed: 38923761google scholar: lookup
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