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Home / Equine Research Bank / J Cell Physiol / Equine umbilical cord blood contains a population of stem ce...
Journal of cellular physiology2007; 215(2); 329-336; doi: 10.1002/jcp.21312

Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.

Abstract: Mesenchymal stem cells (MSCs) offer promise as therapeutic aids in the repair of tendon, ligament, and bone damage suffered by sport horses. The objective of the study was to identify and characterize stem-like cells from newborn foal umbilical cord blood (UCB). UCB was collected and MSC isolated using human reagents. The cells exhibit a fibroblast-like morphology and express the stem cell markers Oct4, SSEA-1, Tra1-60 and Tra1-81. Culture of the cells in tissue-specific differentiation media leads to the formation of cell types characteristic of mesodermal and endodermal origins. Chondrogenic differentiation reveals proteoglycan and glycosaminoglycan synthesis as measured histochemically and Sox9 and collagen 2A1 gene transcription. Osteocytes capable of mineral deposition, osteonectin and Runx2 transcription were evident. Hepatogenic cells formed from UCBs express albumin and cytokeratin 18. Multinucleated myofibers that express desmin were observed indicating partial differentiation into mature muscle cells. Interestingly, conventional human protocols for UCB differentiation into adipocytes were unsuccessful in foal UCB and adult horse adipose-derived MSC. These results demonstrate that equine UCB can be induced to form multiple cell types that underlie their value for regenerative medicine in injured horses. In addition, this work suggests that subtle differences exist between equine and human UCB stem cells.
(c) 2007 Wiley-Liss, Inc.
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Publication Date: 2007-10-12 PubMed ID: 17929245DOI: 10.1002/jcp.21312Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research article explores how umbilical cord blood (UCB) from newborn foals contains stem cells that could potentially be used for treatments in regenerative medicine, specifically for repairing damaged pads and ligaments in sport horses.

Research Objective

  • The main purpose of the research was to identify and study the characteristic features of stem-like cells found in the UCB of newborn foals. The researchers were particularly interested in their potential therapeutic use for the treatment of injuries in sport horses.

Methodology

  • The researchers collected UCB and isolated the Mesenchymal stem cells (MSCs) using human reagents. They closely examined the morphology of these cells and their expression of specific stem cell markers like Oct4, SSEA-1, Tra1-60, and Tra1-81.
  • The cells were then cultured in tissue-specific differentiation media. This led to the formation of cell types characteristic of mesodermal and endodermal origins – necessary for their potential use in regenerative therapies.

Findings

  • The research found that these stem-like cells could form various cell types such as osteocytes (responsible for bone formation) and hepatogenic cells (related to liver function). Their ability to form various cell types affirms their potential usability for regenerative medicine.
  • They also found that these cells could form multilocular myofibers, which constitute a fundamental component of muscle structure.
  • However, they failed to use conventional human protocols to differentiate the UCB stem cells into adipocytes (fat cells), both in foal UCB and adult horse adipose-derived MSCs, indicating the existence of differences between human and equine stem cells.

Conclusion

  • The results of this study demonstrated the potential of equine UCB stem cells to form various types of cells, thereby endorsing their potential value in regenerative medicine, especially in treating injured horses.
  • However, the study also suggested the existence of subtle differences between human and equine stem cells, implying that different protocols may be needed if these equine MSCs are to be practically used for regenerative treatments.

Cite This Article

APA
Reed SA, Johnson SE. (2007). Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types. J Cell Physiol, 215(2), 329-336. https://doi.org/10.1002/jcp.21312

Publication

Journal of cellular physiology
ISSN: 1097-4652
NlmUniqueID: 0050222
Country: United States
Language: English
Volume: 215
Issue: 2
Pages: 329-336

Researcher Affiliations

Reed, Sarah A
  • Department of Animal Sciences, University of Florida, Gainesville, Florida 32611, USA.
Johnson, Sally E

    MeSH Terms

    • Adipocytes / cytology
    • Animals
    • Animals, Newborn
    • Biomarkers / metabolism
    • Cell Differentiation
    • Cells, Cultured
    • Chondrocytes / cytology
    • Endoderm / cytology
    • Fetal Blood / cytology
    • Horses
    • Mesoderm / cytology
    • Muscle Cells / cytology
    • Octamer Transcription Factor-3 / metabolism
    • Osteocytes / cytology
    • Stem Cells / cytology
    • Stem Cells / metabolism

    Citations

    This article has been cited 30 times.
    1. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
      doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
    2. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
      doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
    3. Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses. Stem Cells Cloning 2018;11:13-22.
      doi: 10.2147/SCCAA.S151763pubmed: 29497320google scholar: lookup
    4. Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX. Isolation and characterization of equine endometrial mesenchymal stromal cells. Stem Cell Res Ther 2017 Jul 12;8(1):166.
      doi: 10.1186/s13287-017-0616-0pubmed: 28701175google scholar: lookup
    5. Zahedi M, Parham A, Dehghani H, Mehrjerdi HK. Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells. Int J Stem Cells 2017 May 30;10(1):93-102.
      doi: 10.15283/ijsc16036pubmed: 28222255google scholar: lookup
    6. Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors. PLoS One 2016;11(10):e0164049.
      doi: 10.1371/journal.pone.0164049pubmed: 27732616google scholar: lookup
    7. Lee S, Jeong S, Lee C, Oh J, Kim SC. Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner. Stem Cells Int 2016;2016:2142646.
      doi: 10.1155/2016/2142646pubmed: 27630717google scholar: lookup
    8. Somal A, Bhat IA, B I, Pandey S, Panda BS, Thakur N, Sarkar M, Chandra V, Saikumar G, Sharma GT. A Comparative Study of Growth Kinetics, In Vitro Differentiation Potential and Molecular Characterization of Fetal Adnexa Derived Caprine Mesenchymal Stem Cells. PLoS One 2016;11(6):e0156821.
      doi: 10.1371/journal.pone.0156821pubmed: 27257959google scholar: lookup
    9. Shikh Alsook MK, Gabriel A, Piret J, Waroux O, Tonus C, Connan D, Baise E, Antoine N. Tissues from equine cadaver ligaments up to 72 hours of post-mortem: a promising reservoir of stem cells. Stem Cell Res Ther 2015 Dec 18;6:253.
      doi: 10.1186/s13287-015-0250-7pubmed: 26684484google scholar: lookup
    10. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood. Cytotechnology 2016 Aug;68(4):795-807.
      doi: 10.1007/s10616-014-9831-zpubmed: 25487085google scholar: lookup
    11. Corradetti B, Correani A, Romaldini A, Marini MG, Bizzaro D, Perrini C, Cremonesi F, Lange-Consiglio A. Amniotic membrane-derived mesenchymal cells and their conditioned media: potential candidates for uterine regenerative therapy in the horse. PLoS One 2014;9(10):e111324.
      doi: 10.1371/journal.pone.0111324pubmed: 25360561google scholar: lookup
    12. Taha MF, Javeri A, Rohban S, Mowla SJ. Upregulation of pluripotency markers in adipose tissue-derived stem cells by miR-302 and leukemia inhibitory factor. Biomed Res Int 2014;2014:941486.
      doi: 10.1155/2014/941486pubmed: 25147827google scholar: lookup
    13. Cadby JA, Buehler E, Godbout C, van Weeren PR, Snedeker JG. Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair. PLoS One 2014;9(3):e92474.
      doi: 10.1371/journal.pone.0092474pubmed: 24651449google scholar: lookup
    14. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res Ther 2014 Feb 21;5(1):25.
      doi: 10.1186/scrt414pubmed: 24559797google scholar: lookup
    15. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar P, Somasundaram RK, Kumar S. Immunophenotypic characterization and tenogenic differentiation of mesenchymal stromal cells isolated from equine umbilical cord blood. In Vitro Cell Dev Biol Anim 2014 Jun;50(6):538-48.
      doi: 10.1007/s11626-013-9729-7pubmed: 24414976google scholar: lookup
    16. Rutigliano L, Corradetti B, Valentini L, Bizzaro D, Meucci A, Cremonesi F, Lange-Consiglio A. Molecular characterization and in vitro differentiation of feline progenitor-like amniotic epithelial cells. Stem Cell Res Ther 2013 Oct 30;4(5):133.
      doi: 10.1186/scrt344pubmed: 24405576google scholar: lookup
    17. Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells. Stem Cells Cloning 2008;1:1-9.
      doi: 10.2147/sccaa.s3824pubmed: 24198500google scholar: lookup
    18. Reed SA, Johnson SE. Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation. Cytotechnology 2014 Jan;66(1):27-35.
      doi: 10.1007/s10616-012-9533-3pubmed: 23299298google scholar: lookup
    19. Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S. Isolation and characterization of equine amnion mesenchymal stem cells. Cell Biol Int Rep (2010) 2011 Sep 13;18(1):e00011.
      doi: 10.1042/CBR20110004pubmed: 23124164google scholar: lookup
    20. Spaas JH, Guest DJ, Van de Walle GR. Tendon regeneration in human and equine athletes: Ubi Sumus-Quo Vadimus (where are we and where are we going to)?. Sports Med 2012 Oct 1;42(10):871-90.
      doi: 10.1007/BF03262300pubmed: 22963225google scholar: lookup
    21. Cardoso TC, Ferrari HF, Garcia AF, Novais JB, Silva-Frade C, Ferrarezi MC, Andrade AL, Gameiro R. Isolation and characterization of Wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system. BMC Biotechnol 2012 May 4;12:18.
      doi: 10.1186/1472-6750-12-18pubmed: 22559872google scholar: lookup
    22. Violini S, Gorni C, Pisani LF, Ramelli P, Caniatti M, Mariani P. Isolation and differentiation potential of an equine amnion-derived stromal cell line. Cytotechnology 2012 Jan;64(1):1-7.
      doi: 10.1007/s10616-011-9398-xpubmed: 21994048google scholar: lookup
    23. Corradetti B, Lange-Consiglio A, Barucca M, Cremonesi F, Bizzaro D. Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix. Cell Prolif 2011 Aug;44(4):330-42.
      doi: 10.1111/j.1365-2184.2011.00759.xpubmed: 21645152google scholar: lookup
    24. Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells. Vet Res Commun 2011 Aug;35(6):355-65.
      doi: 10.1007/s11259-011-9480-zpubmed: 21614641google scholar: lookup
    25. Fite BZ, Decaris M, Sun Y, Sun Y, Lam A, Ho CK, Leach JK, Marcu L. Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging. Tissue Eng Part C Methods 2011 Apr;17(4):495-504.
      doi: 10.1089/ten.tec.2010.0368pubmed: 21303258google scholar: lookup
    26. Lovati AB, Corradetti B, Lange Consiglio A, Recordati C, Bonacina E, Bizzaro D, Cremonesi F. Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells. Vet Res Commun 2011 Feb;35(2):103-21.
      doi: 10.1007/s11259-010-9457-3pubmed: 21193959google scholar: lookup
    27. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor. Vet Res Commun 2009 Dec;33(8):811-21.
      doi: 10.1007/s11259-009-9229-0pubmed: 19472068google scholar: lookup
    28. Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine. Can Vet J 2009 Feb;50(2):155-65.
      pubmed: 19412395
    29. Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12. BMC Cell Biol 2009 Apr 22;10:29.
      doi: 10.1186/1471-2121-10-29pubmed: 19383177google scholar: lookup
    30. Purbantoro SD, Taephatthanasagon T, Purwaningrum M, Hirankanokchot T, Peralta S, Fiani N, Sawangmake C, Rattanapuchpong S. Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine. Front Vet Sci 2024;11:1325559.
      doi: 10.3389/fvets.2024.1325559pubmed: 38450027google scholar: lookup
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