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Home / Equine Research Bank / J Vet Diagn Invest / Equine urinary N-acetyl-β-D-glucosaminidase assay vali...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2019; 31(5); 688-695; doi: 10.1177/1040638719867124

Equine urinary N-acetyl-β-D-glucosaminidase assay validation and correlation with other markers of kidney injury.

Abstract: Detection of equine acute kidney injury (AKI) is hindered by limited markers of early renal damage in horses. N-acetyl-β-D-glucosaminidase (NAG), a lysosomal enzyme in renal tubular epithelium released into urine during tubular insult, has shown promise for early identification of AKI in humans and other species. We validated an assay for NAG in equine urine and measured urinary NAG in 7 azotemic and 7 non-azotemic client-owned adult horses. The enzymatic NAG assay was validated using within- and between-run coefficients of variation (CVs), recovery following standard addition, and linearity of dilution. Intra- and inter-run CVs (21% and 3.2%, respectively), average recovery following standard addition (99-109%), and linearity under serial dilution ( = 0.997) were satisfactory. Urine NAG index was significantly correlated with urinary fractional excretion of sodium (FE; ρ = 0.76, < 0.001) and plasma creatinine (ρ = 0.74, = 0.001). Median urine NAG indices were higher in azotemic horses ( = 0.006), in horses with increased urinary FE ( = 0.006), and in horses with increased urine gamma-glutamyl transferase index ( = 0.032). Urine NAG can be measured in horses and shows positive correlation with 2 current renal biomarkers. Additional work is needed to establish normal equine reference intervals and characterize the increase of urine NAG index in horses in relation to tubular injury.
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Publication Date: 2019-07-26 PubMed ID: 31347464PubMed Central: PMC6727119DOI: 10.1177/1040638719867124Google Scholar: Lookup
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  • Journal Article
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the potential of N-acetyl-β-D-glucosaminidase (NAG) as a potential marker for early detection of acute kidney injury (AKI) in horses. It presents the successful validation of a NAG assay in equine urine with significantly higher urine NAG indices observed in azotemic horses.

Objective of the Study

  • The research is primarily aimed at recognizing and investigating a potential marker for detecting early renal damages in horses, as the current procedures available have limitations.
  • The study focuses on the lysosomal enzyme N-acetyl-β-D-glucosaminidase (NAG), which is released into urine during kidney tubular damage and has shown promise as a potential early marker for acute kidney injury (AKI) in diverse species, including humans.

Methodology and Validation of the NAG Assay

  • The researchers use an enzymatic NAG assay specifically for equine urine. The assay’s validation involves evaluating its within- and between-run coefficients of variation (CVs), its recovery rate following add-on standards, and its dilution linearity.
  • Outcomes from the assay validation were determined satisfactory, with intra- and inter-run coefficients of variation at 21% and 3.2% respectively, recovery following standard addition averaging at around 99-109%, and proven linearity under serial dilution (r=0.997).

Study Findings and Correlation with Other Renal Biomarkers

  • Urinary NAG was measured in both azotemic and non-azotemic adult horses. Results have shown significant urine NAG index correlations with urinary fractional excretion of sodium (FE) and plasma creatinine.
  • It was found that the median urine NAG indices were notably higher in azotemic horses, as well as in those manifesting increased urinary FE and urine gamma-glutamyl transferase index.

Conclusions and Further Study

  • The findings posited that urine NAG can be reliably measured in horses, and has shown possible correlation with current renal biomarkers.
  • While the research results prove promising for urine NAG as a potential early renal damage marker, more research is necessary to determine the normal equine reference intervals for NAG, and to fully understand its increase in relation to tubular injury in horses.

Cite This Article

APA
Bayless RL, Moore AR, Hassel DM, Byer BJ, Landolt GA, Nout-Lomas YS. (2019). Equine urinary N-acetyl-β-D-glucosaminidase assay validation and correlation with other markers of kidney injury. J Vet Diagn Invest, 31(5), 688-695. https://doi.org/10.1177/1040638719867124

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 31
Issue: 5
Pages: 688-695

Researcher Affiliations

Bayless, Rosemary L
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).
Moore, A Russell
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).
Hassel, Diana M
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).
Byer, Brittney J
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).
Landolt, Gabriele A
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).
Nout-Lomas, Yvette S
  • College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Bayless).
  • College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (Moore, Hassel, Byer, Landolt, Nout-Lomas).

MeSH Terms

  • Acetylglucosaminidase / urine
  • Acute Kidney Injury / diagnosis
  • Acute Kidney Injury / urine
  • Acute Kidney Injury / veterinary
  • Animals
  • Biomarkers / urine
  • Creatinine / blood
  • Female
  • Horse Diseases / diagnosis
  • Horse Diseases / urine
  • Horses
  • Humans
  • Male
  • Sodium / urine
  • gamma-Glutamyltransferase / urine

Conflict of Interest Statement

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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Citations

This article has been cited 4 times.
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