Equivalent seminal characteristics in human and stallion at first and second ejaculated fractions.
Abstract: Sperm quality was assessed in normozoospermic human (n = 10) and Spanish breed stallion (n = 10) after sperm fractionation during ejaculation. The first ejaculated fraction was separated from the second. A third sample was reconstituted using equivalent proportion of both fractions (RAW). Fraction 1, Fraction 2 and RAW semen were incubated for 30 min at 37°C to homogenise the impact of iatrogenic damage between both species. Sperm concentration, motility and sperm DNA damage were assessed in each fraction and RAW semen. The results showed two important facts: (i) spermatozoa confined at Fraction 1 exhibit superior parameters than those included at Fraction 2 in both species, and (ii) there is a certain level of concordance between species in the proportion of benefit observed when Fraction 1 is compared to RAW semen. Altogether, these results call into question whether the standard practice of whole ejaculate collection can be considered the best strategy when using male gametes for artificial insemination. In fact, the reconstituted RAW semen exhibits poorer semen characteristics than those found in Fraction 1.
© 2016 Blackwell Verlag GmbH.
Publication Date: 2016-09-23 PubMed ID: 27660115DOI: 10.1111/and.12708Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates sperm quality in both humans and stallions by separating and studying the first and second fractions of ejaculation. The results suggest that the standard practice of whole ejaculate collection may not be the best approach in artificial insemination, as sperm from the initial fraction display superior characteristics.
Study Design and Methods
- The study involved ten normozoospermic human participants and ten Spanish breed stallions. Normozoospermic refers to men with a normal amount of sperm within their semen.
- The sperm was fractionated during ejaculation, dividing it into the first and second ejaculated fractions. A third sample, referred to as RAW, was reconstituted using equal proportions of both fractions.
- To minimize any potential impact of iatrogenic damage (harm caused by medical examination or treatment), each of the fractions and the RAW semen were incubated for thirty minutes at 37 degrees Celsius. This assured a uniform environment for the sperm, regardless of the species.
Assessments Made
- The assessments made included sperm concentration, motility (ability to move), and DNA damage in each of the fractions and in the RAW semen.
Key Findings
- The sperm present in the first fraction of ejaculate exhibited superior characteristics as compared to those found in the second fraction, in both humans and stallions.
- There was a notable parallel between the two species with regards the benefits observed when comparing the first fraction to the reconstituted RAW semen.
Implications of the Findings
- The results raised questions about the efficacy of the traditional practice of collecting the entire ejaculate for the purpose of artificial insemination.
- Remarkably, the reconstituted RAW semen displayed poorer semen characteristics as compared to those observed in the first fraction. This suggests that utilizing the whole ejaculate sample might not offer the best results for artificial insemination practices.
Cite This Article
APA
de la Torre J, Sánchez-Martín P, Gosálvez J, Crespo F.
(2016).
Equivalent seminal characteristics in human and stallion at first and second ejaculated fractions.
Andrologia, 49(8).
https://doi.org/10.1111/and.12708 Publication
Researcher Affiliations
- Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain.
- Clínica Ginemed, Sevilla, Spain.
- Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain.
- Departamento de Reproducción, Centro Militar de Cría Caballar (FESCCR, Ministerio de Defensa), Ávila, Spain.
MeSH Terms
- Animals
- DNA Damage / physiology
- Ejaculation / physiology
- Horses
- Humans
- Insemination, Artificial / methods
- Insemination, Artificial / veterinary
- Male
- Semen / cytology
- Semen Analysis
- Sperm Motility / physiology
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 5 times.- Tanaka T, Kojo K, Nagumo Y, Ikeda A, Shimizu T, Fujimoto S, Kakinuma T, Uchida M, Kimura T, Kandori S, Negoro H, Nishiyama H. A new clustering model based on the seminal plasma/serum ratios of multiple trace element concentrations in male patients with subfertility. Reprod Med Biol 2024 Jan-Dec;23(1):e12584.
- Viñolas-Vergés E, Ribas-Maynou J, Barranco I, Peres Rubio C, Bonet S, Roca J, Yeste M. Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction. J Anim Sci Biotechnol 2023 Nov 6;14(1):139.
- Rodriguez-Martinez H, Martinez EA, Calvete JJ, Peña Vega FJ, Roca J. Seminal Plasma: Relevant for Fertility?. Int J Mol Sci 2021 Apr 22;22(9).
- Mateo-Otero Y, Fernández-López P, Gil-Caballero S, Fernandez-Fuertes B, Bonet S, Barranco I, Yeste M. (1)H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites. Biomolecules 2020 Jun 15;10(6).
- Tvrdá E, Arroyo F, Gosálvez J. Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation. Int Urol Nephrol 2018 Aug;50(8):1381-1388.
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