Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study.
Abstract: Horse blood has a higher tendency to form red blood cell (RBC) aggregates compared with human blood, with this enhanced aggregation previously attributed to differences in plasma factors. Our results confirm this observation and further indicate that washed horse RBC also have a significantly higher aggregation tendency in dextran 70 solutions (i.e., horse RBC have a higher "aggregability"). In contrast, the aggregation tendency of rat RBC, both in autologous plasma and in dextran 70, is significantly less compared with human and horse RBC. Other rheological findings for horse and rat RBC include smaller changes in RBC deformation indexes over the same shear stress range and a lower RBC shape recovery time constant. Rat RBC also had higher two-phase aqueous polymer partition coefficients, suggesting a higher surface charge. Membrane protein analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed marked differences: 1) band 4.2 protein was lacking in horse RBC membranes, and 2) carbohydrate groups have different distributions in human, rat, and horse RBC, as indicated by different patterns in periodic acid-Schiff-stained protein bands. Our results clearly indicate significant differences in RBC aggregability among the three species and indicate that cellular factors contribute importantly to these differences. Furthermore, they suggest that systematic studies of blood and RBC from different species should provide insight into the mechanism(s) of RBC aggregation.
Publication Date: 1998-01-22 PubMed ID: 9435593DOI: 10.1152/ajpheart.1997.273.6.H2604Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research study investigates the nature of red blood cell (RBC) aggregation in horse, human, and rat blood. It finds that horse blood shows a higher tendency of RBC aggregation compared to human and rat blood, and contains significant variations in cellular properties that contribute to these differences.
Objective and Methodology
- The study’s objective was to investigate and compare the aggregation tendencies and cellular properties of RBCs in horse, human, and rat blood.
- The researchers conducted their examinations using a solution of dextran 70 and also analysed the membrane protein composition of the RBCs using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Key Findings
- Horse RBCs exhibited a higher tendency of aggregability across both plasma and dextran 70 solutions when compared to human and rat RBCs.
- Rat RBCs, on the other hand, showed a lower tendency to aggregate in these solutions when compared to human or horse RBCs.
- Horse and rat RBCs also showed smaller variances in their deformation indexes over the same shear stress range as well as displayed a lower RBC shape recovery time constant compared to human RBCs.
- Rat RBCs exhibited higher two-phase aqueous polymer partition coefficients, a characteristic hinting at a higher surface charge.
Comparison of Cellular Properties
- The membrane protein analysis showed notable differences between the three species’ RBCs- horse RBC membranes lacked ‘band 4.2 protein’ which is otherwise present in human and rat RBC membranes.
- The different patterns of periodic acid-Schiff-stained protein bands suggested the distributions of carbohydrate groups within the RBCs vary across horse, human, and rat blood.
Conclusion and Recommendations
- These results evidence significant differences in RBC aggregability among the three species, indicating that cellular factors play a substantial role in these differences.
- Furthermore, the researchers suggest that further systematic studies investigating blood and RBCs from different species would provide more insights into the mechanisms of RBC aggregation.
Cite This Article
APA
Baskurt OK, Farley RA, Meiselman HJ.
(1998).
Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study.
Am J Physiol, 273(6), H2604-H2612.
https://doi.org/10.1152/ajpheart.1997.273.6.H2604 Publication
Researcher Affiliations
- Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033, USA.
MeSH Terms
- Animals
- Dextrans / pharmacology
- Erythrocyte Aggregation / drug effects
- Erythrocyte Count
- Erythrocyte Deformability
- Erythrocyte Membrane / chemistry
- Erythrocytes / drug effects
- Erythrocytes / physiology
- Female
- Hematocrit
- Hemoglobins / analysis
- Horses
- Humans
- Male
- Membrane Proteins / blood
- Membrane Proteins / isolation & purification
- Rats
- Species Specificity
Grant Funding
- GM-28673 / NIGMS NIH HHS
- HL-15722 / NHLBI NIH HHS
- HL-48484 / NHLBI NIH HHS
Citations
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