Evaluation of a haematological analyser (Sysmex F-800) with equine blood.
Abstract: A semiautomatic electronic blood cell counter (Sysmex F-800) was evaluated with equine blood, according to the protocol of the International Committee for Standardization in Haematology (ICSH, 1984). The precision and overall reproducibility were acceptable for all the parameters studied except for the platelet count, in which a coefficient of variation of 18.8% and 21.7% was obtained for within and between batch precision and 26.76% for overall reproducibility. Carry-over for the haematocrit value and platelet count was unsatisfactory, thus the use of a blank diluent sample between different analyses was necessary. Linearity of the analyser was acceptable in the range studied. A total of 100 equine blood samples were studied with the Sysmex F-800 and the manual method. The correlations between both Methods were acceptable for all the parameters. In the storage study, the red blood cell count and the haemoglobin concentration were the parameters with longest stability (72 h) at 4 degrees C and 25 degrees C. Platelet count decreased significantly at 3 h post-extraction in those samples stored at 4 degrees C. During the evaluation period, Sysmex F-800 was easy to handle and it is well suited for routine equine blood cell analysis.
Publication Date: 1998-05-20 PubMed ID: 9591475DOI: 10.1111/j.1439-0442.1998.tb00807.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Analytical Methods
- Blood
- Blood Analysis
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Correlation Analysis
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Equine Diseases
- Equine Health
- Erythrocytes
- Hematology
- Hemoglobin
- Horses
- Laboratory Methods
- Platelets
- Reproducibility of Results
- Veterinary Medicine
- Veterinary Research
Summary
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The research assesses the performance of an electronic blood cell counter (Sysmex F-800) using horse blood, focusing on its accuracy, reliability, and ease of use. The evaluation reports that the device performs well in most areas, but requires improvement with regard to the platelet count, and additional diluent samples between different analyses.
Evaluation Methodology
- The Sysmex F-800 electronic blood cell counter’s performance was evaluated using equine (horse) blood as the sample.
- The evaluation process adhered to the protocol set by the International Committee for Standardization in Haematology (ICSH, 1984).
- A total of 100 equine blood samples were used for the study, testing them both with the Sysmex F-800 and manually to compare the results.
Performance Metrics
- The research focused on the device’s precision, overall reproducibility, linearity, and storage capabilities.
- Its precision and overall reproducibility were deemed acceptable for all parameters except the platelet count.
- The reproducibility of the platelet count recorded a coefficient of variation of 18.8% and 21.7% for within and between batch precision, and 26.76% for overall reproducibility.
Handling of Inter-Analysis Carry-Over Effects
- The Sysmex F-800 showed carry-over effects with the haematocrit value and platelet count.
- To mitigate this, it was necessary to use a blank diluent sample between different analyses.
Linearity and Storage Study
- The linearity of the analyser, which relates to its accuracy over a range of measurements, was found to be acceptable within the range studied.
- The storage study found that the red blood cell count and the haemoglobin concentration were the parameters with the longest stability (72 hours) at 4 degrees Celsius and 25 degrees Celsius.
- The platelet count decreased significantly at 3 hours post-extraction when the samples were stored at 4 degrees Celsius.
Usability of the Sysmex F-800
- The research concluded that the Sysmex F-800 was user-friendly and suitable for routine equine blood cell analysis.
Cite This Article
APA
Pastor J, Cuenca R, Velarde R, Marco I, Viñas L, Lavín S.
(1998).
Evaluation of a haematological analyser (Sysmex F-800) with equine blood.
Zentralbl Veterinarmed A, 45(2), 119-126.
https://doi.org/10.1111/j.1439-0442.1998.tb00807.x Publication
Researcher Affiliations
- Departamento de Patología y Producciones Animales (U.D. Patología General y Médica), Facultad de Veterinaria, Universidad Autónoma de Barcelona, Spain.
MeSH Terms
- Animals
- Autoanalysis / instrumentation
- Autoanalysis / methods
- Autoanalysis / veterinary
- Blood Cell Count / veterinary
- Blood Chemical Analysis / instrumentation
- Blood Chemical Analysis / methods
- Blood Chemical Analysis / veterinary
- Hematology / instrumentation
- Hematology / methods
- Horses / blood
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 1 times.- Moxon R, Dutton Worsfold R, Davis J, Adams W, England GCW. Luteal phase decrease in packed cell volume in healthy non-pregnant and pregnant bitches. Vet Med Sci 2023 Sep;9(5):1989-1997.
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