Evaluation of a laparoscopic technique for collection of serial full-thickness small intestinal biopsy specimens in standing sedated horses.

The research evaluates a laparoscopic technique that gathers serial full-thickness small intestinal biopsy specimens in horses. The technique proves promising as it allows cereal collection with minimal complications.

Methodology

The study comprised two parts.

• The ex vivo part of the study involved dissecting sections of duodenum and jejunum from six horses immediately after euthanasia. Each section was divided into three and assigned to three groups: The control group, the double-layer hand-sewn closure group, and the endoscopic linear stapler (ELS) group. Biopsy impact was measured, including elements such as bursting strength, bursting wall tension and luminal diameter reduction.
• The in vivo portion of the study comprised seven horses with monthly interval laparoscopic collection of small intestinal biopsy specimens. Biopsies from the descending duodenum and the distal part of the jejunum were taken using an ELS while the horses were sedated and standing. The effectiveness of this method was evaluated based on biopsy suitability for histologic examination.

Results

The findings showed notable variance between the groups in the ex vivo portion. Mean bursting strength and bursting wall tension were significantly less in the ELS group than in the double-layer hand-sewn closure and control groups in both the duodenal and jejunal segments. Further, the hand-sewn closure technique at the biopsy site reduced luminal diameter more than the stapling technique.

In regard to the in vivo part of the study, all biopsy specimens collected were apt for histologic examination. Importantly, no significant perioperative complications arose in the laparoscopic procedures, highlighting the technique’s safety.

Conclusion

Based on the findings, the laparoscopic collection of serial full-thickness small intestinal biopsy specimens using a 45mm ELS was hailed as a safe and effective technique in healthy adult experimental horses. This approach could hold significant potential in veterinary medicine by allowing serial sample collection with minimal discomfort and complications.