Evaluation of a single intra-articular injection of autologous protein solution for treatment of osteoarthritis in horses.
Abstract: To evaluate intra-articular autologous protein solution (APS) for the treatment of osteoarthritis in horses. Animals-40 client-owned horses with naturally occuring osteoarthritis. Methods: APS was generated from a dual-device system that concentrated plasma and WBC proteins and enriched platelet growth factors. Horses were randomly assigned to receive an intra-articular injection of 5 mL of saline (0.9% NaCl) solution (n = 20) or APS (20), exercised on a treadmill, and evaluated on the basis of lameness grades, kinetic gait analysis, joint circumference, and range of motion for 14 days. Horses that received saline solution were administered APS at termination of the study, and clients scored horses for lameness and discomfort before, 12 weeks after, and 52 weeks after the APS injection. Results: The APS group had significant improvements in lameness grade, asymmetry indices of vertical peak force, and range of joint motion by 14 days, compared with baseline or control group values. No adverse effects associated with APS treatment were evident. Clients assessed lameness and comfort as improved at 12 and 52 weeks. The APS had greater likelihood (OR, 4.3 to 30.0) of a therapeutic response in horses with a lameness score < 4, < 10% vertical force asymmetry, or absence of marked osteophyte formation, subchondral sclerosis, or joint space narrowing. Concentration of interleukin-1 receptor antagonist in APS was 5.8 times that in blood. Conclusions: Intra-articular administration of APS can be considered an effective treatment option for equine osteoarthritis, with the potential for disease-modifying effects.
Publication Date: 2014-01-30 PubMed ID: 24471750DOI: 10.2460/ajvr.75.2.141Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the effectiveness of a single intra-articular injection of autologous protein solution (APS) as a treatment option for horses suffering from osteoarthritis. The results demonstrate clear improvement in lameness, comfort, and range of joint motion without any adverse side effects.
Study Design and Procedure
- The study sample consisted of 40 client-owned horses diagnosed with naturally occurring osteoarthritis. These horses were randomly allocated to two groups; one group received 5 mL of saline (0.9% NaCl) solution injection and the other received APS.
- The APS was created using a special dual-device system that focused on the concentration of plasma and white blood cell proteins in addition to enriching platelet growth factors.
- After the administration of the respective injections, the horses underwent exercise on a treadmill and were evaluated based on lameness grades, kinetic gait analysis, joint circumference, and range of motion for a duration of 14 days.
- At the end of the study, the group that initially received saline solution was administered APS. The horse owners were asked to score their horses for lameness and discomfort before and 12 weeks as well as 52 weeks after receiving the APS injection.
Results and Findings
- The results demonstrated that horses in the APS group showed significant improvements in lameness grade, asymmetry indices of the vertical peak force, and joint motion range by the end of the 14-day period, as compared to baseline or control group values.
- No adverse effects were observed to be associated with APS treatment.
- The clients evaluated lameness and comfort to be improved after 12 and 52 weeks post-APS injection.
- Horses exhibiting a lameness score less than 4, less than 10% vertical force asymmetry, or absence of marked osteophyte formation, subchondral sclerosis, or joint space narrowing were found to have a higher likelihood (odds ratio ranging from 4.3 to 30.0) of a therapeutic response to APS treatment.
- From a molecular perspective, interleukin-1 receptor antagonist concentration in APS was reported to be 5.8 times that present in blood, pointing towards a potential mechanism through which APS may relieve symptoms of osteoarthritis.
Conclusion
- The study concludes that using APS as an intra-articular treatment can be an effective strategy for managing equine osteoarthritis. Moreover, APS has the potential to modify the disease process, reducing symptoms, and improving the quality of life for affected horses.
Cite This Article
APA
Bertone AL, Ishihara A, Zekas LJ, Wellman ML, Lewis KB, Schwarze RA, Barnaba AR, Schmall ML, Kanter PM, Genovese RL.
(2014).
Evaluation of a single intra-articular injection of autologous protein solution for treatment of osteoarthritis in horses.
Am J Vet Res, 75(2), 141-151.
https://doi.org/10.2460/ajvr.75.2.141 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.
MeSH Terms
- Animals
- Blood Proteins / administration & dosage
- Blood Proteins / therapeutic use
- Female
- Gait / physiology
- Horse Diseases / drug therapy
- Horses
- Injections, Intra-Articular / veterinary
- Lameness, Animal
- Leukocytes / chemistry
- Male
- Osteoarthritis / drug therapy
- Osteoarthritis / veterinary
- Pain / drug therapy
- Pain / veterinary
- Synovial Fluid
Citations
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