Evaluation of a turbidimetric immunoassay for measurement of plasma IgG concentration in foals.
Abstract: To validate a turbidimetric immunoassay (TIA) for measurement of plasma IgG concentrations in foals. Methods: 36 foals. Methods: Blood samples were collected from foals before suckling and at 12 and 24 to 36 hours after birth. Plasma IgG concentrations were determined via a commercial single radial immunodiffusion (RID) assay. By use of goat anti-equine IgG antiserum and a spectrophotometer, a TIA was developed to measure plasma and serum IgG concentrations; the percentage light transmission was calibrated against RID assay-determined IgG concentrations. Assay repeatability and effects of serial dilution, sample type, and ambient temperature on assay results were evaluated. Results: Serial dilution of plasma samples from foals 12 and 24 to 36 hours of age with presuckle plasma yielded percentage light transmission results that were highly inversely correlated (r = -0.95) with IgG concentrations determined via RID assay. Measurements of IgG in plasma and serum samples via TIA did not differ. When samples were assayed multiple times, the coefficient of variation was 90%; specificity was 99.1% and 70.5%, respectively; and positive and negative predictive values were 98.1% and 71.5%, respectively, and 96.4% and 91.1%, respectively. Conclusions: Plasma IgG concentrations in foals determined via the TIA and RID assay were highly correlated. The TIA rapidly yielded quantitative results and would be useful in clinical situations where intervention decisions are time dependent.
Publication Date: 2007-09-04 PubMed ID: 17764416DOI: 10.2460/ajvr.68.9.1005Google Scholar: Lookup
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- Comparative Study
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research validates a method, called turbidimetric immunoassay (TIA), for measuring IgG plasma concentrations in newborn horses (foals). The study found that the TIA method quickly delivered verifiable results, making it a valuable tool for making time-sensitive clinical interventions.
Objective and Methodology
- The study aimed to ascertain the validity of a turbidimetric immunoassay (TIA) for quantifying plasma IgG concentrations in foals.
- 36 foals were used in this experiment. Blood samplings were conducted on the subjects before they were allowed to suckle and at intervals—12 hours and 24 to 36 hours—after birth.
- Commercial single radial immunodiffusion (RID) technique was employed for determining the concentrations of IgG in plasma.
- The researchers developed a TIA using a spectrophotometer and goat anti-equine IgG antiserum. This was done to measure concentrations of IgG in serum and plasma.
- Percentage light transmission was calibrated using RID assay-determined concentrations of IgG.
- The team analyzed the effects of serial dilution, type of sample, and ambient temperature on the TIA results, as well as the repeatability of the assay.
Key Results
- Results from the serial dilution of plasma samples taken from the foals at intervals showed a strong inverse correlation (r=-0.95) between percentage light transmission and the RID assay-determined IgG concentrations.
- The TIA measurements of IgG from plasma and serum samples were identical.
- The researchers observed that the coefficient of variation was less than 5% when the samples were assayed multiple times.
- Ambient temperature was found to have no influence on the TIA results.
- The TIA sensitivity surpassed 90% at IgG concentrations of 400 and 800 mg/dL. Specificity was recorded at 99.1% and 70.5%, respectively. Positive and negative predictive values were placed at 98.1% and 71.5%, and 96.4% and 91.1%, respectively.
Conclusions
- The study confirmed a high correlation between IgG plasma concentrations in foals calculated using TIA and RID assays.
- The TIA produced quantitative results quickly, making it a valuable diagnostic tool for use in time-critical clinical scenarios.
Cite This Article
APA
McCue PM.
(2007).
Evaluation of a turbidimetric immunoassay for measurement of plasma IgG concentration in foals.
Am J Vet Res, 68(9), 1005-1009.
https://doi.org/10.2460/ajvr.68.9.1005 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80525, USA.
MeSH Terms
- Animals
- Animals, Newborn
- Horses / blood
- Horses / immunology
- Immunodiffusion / veterinary
- Immunoglobulin G / blood
- Nephelometry and Turbidimetry / methods
- Nephelometry and Turbidimetry / veterinary
- Predictive Value of Tests
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 2 times.- Ujvari S, Schwarzwald CC, Fouché N, Howard J, Schoster A. Validation of a Point-of-Care Quantitative Equine IgG Turbidimetric Immunoassay and Comparison of IgG Concentrations Measured with Radial Immunodiffusion and a Point-of-Care IgG ELISA. J Vet Intern Med 2017 Jul;31(4):1170-1177.
- Gallacher K, Champion K, Denholm KS. Mare colostrum quality and relationship with foal serum immunoglobulin G concentrations and average daily weight gains. Equine Vet J 2025 Jul;57(4):904-914.
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