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Stem cell research & therapy2016; 7(1); 137; doi: 10.1186/s13287-016-0398-9

Evaluation of amniotic mesenchymal cell derivatives on cytokine production in equine alveolar macrophages: an in vitro approach to lung inflammation.

Abstract: Data obtained in both animal models and clinical trials suggest that cell-based therapies represent a potential therapeutic strategy for lung repair and remodeling. Recently, new therapeutic approaches based on the use of stem cell derivatives (e.g., conditioned medium (CM) and microvesicles (MVs)) to regenerate tissues and improve their functions were proposed. The aim of this study was to investigate the immunomodulatory effects of equine amniotic mesenchymal cell derivatives on lipopolysaccharide (LPS)-induced cytokine production in equine alveolar macrophages, which may be beneficial in lung inflammatory disorders such as recurrent airway obstruction (RAO) in horses. RAO shares many features with human asthma, including an increased number of cells expressing mRNA for interleukin (IL)-4 and IL-5 and a decreased expression of IFN-γ in bronchoalveolar lavage fluid (BALF) of affected horses. The release of TNF-α, IL-6, and TGF-β1 at different time points (1, 24, 48, and 72 h) was measured in equine alveolar macrophages stimulated or not with LPS (10 and 100 ng/mL) in the presence or absence of 10 % CM or 50 × 10(6) MVs/mL. Cytokines were measured using commercially available ELISA kits. For multiple comparisons, analysis of variance was used with Tukey post-hoc test. Differences were considered significant at p ≤ 0.05. Significant modulatory effects of CM on LPS-induced TNF-α release at 24 h, and of both CM and MVs on TNF-α release at 48 h were observed. A trend toward a modulatory effect of both CM and MVs on the release of TGF-β and possibly IL-6 was visible over time. Results support the potential use of CM and MVs in lung regenerative medicine, especially in situations in which TGF-β may be detrimental, such as respiratory allergy. Further studies should evaluate the potential clinical applications of CM and MVs in equine lung diseases, such as RAO and other inflammatory disorders.
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Publication Date: 2016-09-20 PubMed ID: 27651133PubMed Central: PMC5028987DOI: 10.1186/s13287-016-0398-9Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how stem cell derivatives from horse amniotic fluid can alter lung inflammation responses in horse lung cells. It is part of the effort to develop cell-based therapies for repairing lung damage and relieving inflammation in respiratory conditions like asthma.

Research Objective

The research sought to understand if and how derivatives of equine amniotic mesenchymal cells influence cytokine production in horse lung cells when these cells are exposed to lipopolysaccharide (LPS). By looking at the effects on the cytokines TNF-α, IL-6, and TGF-β1, the researchers hoped to gain insights regarding the potential use of these stem cell derivatives in treating lung inflammatory diseases, such as recurrent airway obstruction (RAO) in horses which shares many features with human asthma.

Methodology

  • The researchers used two types of cell derivatives: conditioned medium (CM) and microvesicles (MVs).
  • Their effect was studied in horse lung cells (equine alveolar macrophages) which were or weren’t exposed to LPS, an inflammation trigger.
  • Different LPS concentrations were used and the cells were also exposed to varying amounts of CM or MVs.
  • The release of TNF-α, IL-6, and TGF-β1 was measured at 1, 24, 48, and 72 hours intervals using ELISA kits, a standard tool for measuring proteins in samples.
  • Finally, statistical analysis was conducted to identify any significant differences in cytokine release, due to the various treatments.

Findings

  • The researchers noted that the CM treatment significantly reduced the LPS-induced release of TNF-α at the 24-hour and 48-hour intervals.
  • Both the CM and MVs treatments seemed to reduce the TNF-α release at the 48-hour point.
  • There was a suggestion that both treatments had a modulatory effect on the release of TGF-β and possibly also IL-6, but this needs further exploration.

Implications

The results support the idea that CM and MVs could have therapeutic value in lung pathology, especially in cases where reducing TGF-β could be beneficial, for instance, in respiratory allergies. While promising, these findings serve as preliminary data and the researchers stress the need for further studies to confirm these effects and explore their potential in clinical applications for equine lung diseases and other inflammatory disorders.

Cite This Article

APA
Zucca E, Corsini E, Galbiati V, Lange-Consiglio A, Ferrucci F. (2016). Evaluation of amniotic mesenchymal cell derivatives on cytokine production in equine alveolar macrophages: an in vitro approach to lung inflammation. Stem Cell Res Ther, 7(1), 137. https://doi.org/10.1186/s13287-016-0398-9

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 7
Issue: 1
Pages: 137
PII: 137

Researcher Affiliations

Zucca, Enrica
  • Department of Health, Animal Science and Food Safety (VESPA), Università degli Studi di Milano, Via Celoria 10, 20133, Milano, Italy.
Corsini, Emanuela
  • Laboratory of Toxicology, DiSFeB, Università degli Studi di Milano, Milan, Italy.
Galbiati, Valentina
  • Laboratory of Toxicology, DiSFeB, Università degli Studi di Milano, Milan, Italy.
Lange-Consiglio, Anna
  • Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Lodi, Italy. anna.langeconsiglio@unimi.it.
Ferrucci, Francesco
  • Department of Health, Animal Science and Food Safety (VESPA), Università degli Studi di Milano, Via Celoria 10, 20133, Milano, Italy.

MeSH Terms

  • Amnion / cytology
  • Amnion / immunology
  • Amnion / metabolism
  • Animals
  • Cell-Derived Microparticles / chemistry
  • Cell-Derived Microparticles / immunology
  • Culture Media, Conditioned / chemistry
  • Culture Media, Conditioned / pharmacology
  • Female
  • Horse Diseases / immunology
  • Horse Diseases / pathology
  • Horses
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / immunology
  • Lipopolysaccharides / pharmacology
  • Macrophages, Alveolar / cytology
  • Macrophages, Alveolar / drug effects
  • Macrophages, Alveolar / immunology
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / immunology
  • Mesenchymal Stem Cells / metabolism
  • Models, Biological
  • Pneumonia / immunology
  • Pneumonia / pathology
  • Pneumonia / veterinary
  • Primary Cell Culture
  • Transforming Growth Factor beta1 / biosynthesis
  • Transforming Growth Factor beta1 / immunology
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / immunology

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Citations

This article has been cited 12 times.
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