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Home / Equine Research Bank / Vet J / Evaluation of an automated assay based on monoclonal anti-hu...
Veterinary journal (London, England : 1997)2012; 194(3); 332-337; doi: 10.1016/j.tvjl.2012.05.007

Evaluation of an automated assay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline, and equine SAA.

Abstract: Major acute phase proteins (APPs) have proven diagnostically useful in dogs, cats and horses with routine use facilitated by commercially available automated heterologous assays. An automated assay applicable across all three species would highly facilitate further dissemination of routine use, and the aim of this study was to validate an automated latex agglutination turbidimetric immunoassay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline and equine SAA. Serum samples from 60 dogs, 40 cats and 40 horses were included. Intra- and inter-assay imprecision, linearity and detection limit (DL) were determined to assess analytical performance. To assess clinical performance, equine and feline SAA measurements were compared with parallel measurements using a previously validated automated SAA assay in a method comparison setting, and by assessing overlap performance of canine SAA in healthy dogs and diseased dogs with and without systemic inflammation. Intra- and inter-assay CVs ranged between 1.9-4.6% and between 3.0-14.5%, respectively. Acceptable linearity within a clinically relevant range of SAA concentrations was observed for all three species. The DL was 1.06 mg/L. Method comparison revealed acceptable agreement of the two assays measuring feline and equine SAA, and the overlap performance of canine SAA was acceptable. The tested assay measured SAA in canine, feline and equine serum with analytical and overlap performance acceptable for clinical purposes so improving practical aspects of clinical APP application. The monoclonal nature of the antibodies suggests strong, long-term inter-batch performance stability.
Copyright © 2012 Elsevier Ltd. All rights reserved.
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Publication Date: 2012-06-15 PubMed ID: 22704135DOI: 10.1016/j.tvjl.2012.05.007Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study that validates a multi-species automated assay for measuring Serum Amyloid A (SAA), a major acute phase protein, which could be a useful diagnostic tool in dogs, cats, and horses.

Objectives of the Study

  • The study aimed to validate an automated latex agglutination turbidimetric immunoassay based on monoclonal anti-human serum amyloid A (SAA) antibodies, and its utility in measuring SAA in canine, feline, and equine species.

Methodology

  • 60 dogs, 40 cats and 40 horses, constituting the sample data, provided serum samples for the research.
  • Subsequently, the intra-assay and inter-assay imprecision, linearity, and detection limit of the assay were determined for the purpose of assessing its analytical performance.
  • To evaluate clinical performance, feline and equine SAA measurements were compared with parallel measurements obtained using a previously validated automated SAA assay, and the overlapping performance of canine SAA in both healthy and sick dogs (with or without systemic inflammation) was also assessed.

Findings

  • Intra-assay and inter-assay precision values ranged between 1.9-4.6% and 3.0-14.5% respectively, indicating good reliability of repeated measurements.
  • All three species demonstrated acceptable linearity within a clinically relevant range of SAA concentrations, indicating that the assay readings are proportional to the SAA concentration in the samples.
  • The detection limit (DL) was found to be 1.06 mg/L, suggesting the assay’s ability to detect small quantities of the substance.
  • The comparison between the new SAA assay and a previously validated automated SAA assay showed good agreement for the measurements of feline and equine SAA, validating the accuracy of the new assay.
  • The overlapping performance of canine SAA in healthy and sick dogs was also deemed acceptable.

Conclusion

  • Based on the results of the study, the new automated assay was validated as a measurement tool for SAA in dogs, cats, and horses. It demonstrated acceptable analytical and clinical performance, thereby improving practical aspects of clinical APP application.
  • The monoclonal nature of the antibodies used in the assay underscores the potential for strong, long-term inter-batch performance stability, thus emphasizing it as a reliable tool for ongoing use.

Cite This Article

APA
Christensen M, Jacobsen S, Ichiyanagi T, Kjelgaard-Hansen M. (2012). Evaluation of an automated assay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline, and equine SAA. Vet J, 194(3), 332-337. https://doi.org/10.1016/j.tvjl.2012.05.007

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 194
Issue: 3
Pages: 332-337
PII: S1090-0233(12)00196-7

Researcher Affiliations

Christensen, M
  • Department of Small Animal Clinical Sciences, University of Copenhagen, Denmark. mc@life.ku.dk
Jacobsen, S
    Ichiyanagi, T
      Kjelgaard-Hansen, M

        MeSH Terms

        • Animals
        • Antibodies / blood
        • Automation, Laboratory / instrumentation
        • Automation, Laboratory / methods
        • Cats / metabolism
        • Dog Diseases / blood
        • Dog Diseases / immunology
        • Dogs / metabolism
        • Female
        • Horses / metabolism
        • Humans
        • Immunoassay / instrumentation
        • Immunoassay / methods
        • Immunoassay / veterinary
        • Inflammation / blood
        • Latex Fixation Tests / instrumentation
        • Latex Fixation Tests / methods
        • Latex Fixation Tests / veterinary
        • Limit of Detection
        • Male
        • Nephelometry and Turbidimetry / instrumentation
        • Nephelometry and Turbidimetry / methods
        • Nephelometry and Turbidimetry / veterinary
        • Reproducibility of Results
        • Serum Amyloid A Protein / metabolism

        Citations

        This article has been cited 24 times.
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