Evaluation of articular cartilage progenitor cells for the repair of articular defects in an equine model.
Abstract: We sought to determine the effectiveness of chondroprogenitor cells derived from autologous and allogenic articular cartilage for the repair of cartilage defects in an equine model. Methods: Cartilage defects (15 mm) were created on the medial trochlear ridge of the femur. The following experimental treatments were compared with empty-defect controls: fibrin only, autologous chondroprogenitor cells plus fibrin, and allogenic chondroprogenitor cells plus fibrin (n = 4 or 12 per treatment). Horses underwent strenuous exercise throughout the twelve-month study, and evaluations included lameness (pain) and arthroscopic, radiographic, gross, histologic, and immunohistochemical analyses. Results: Arthroscopy and microscopy indicated that defects in the autologous cell group had significantly better repair tissue compared with defects in the fibrin-only and control groups. Repair tissue quality in the allogenic cell group was not superior to that in the fibrin-only group with the exception of the percentage of type-II collagen, which was greater. Radiographic changes in the allogenic cell group were poorer on average than those in the autologous cell group. Autologous cells significantly reduced central osteophyte formation compared with fibrin alone. Conclusions: On the basis of the arthroscopic, radiographic, and histologic scores, autologous cells in fibrin yielded better results than the other treatments; allogenic cells cannot be recommended at this time.
Copyright © 2015 by The Journal of Bone and Joint Surgery, Incorporated.
Publication Date: 2015-03-20 PubMed ID: 25788305DOI: 10.2106/JBJS.N.00404Google Scholar: Lookup
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Summary
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This research explores the use of cartilage-derived progenitor cells in repairing cartilage defects in horses, showing that autologous cells (from the same individual) were more effective than allogenic ones (from a different individual).
Approach towards Experiment
- This study was carried out using an equine (horse) model. Cartilage defects were artificially induced in horses to evaluate the effectiveness of cartilage-derived progenitor cells, both autologous (coming from the subject itself) and allogenic (coming from a different individual).
- To compare the performance of these cellular treatments, defects were examined post-intervention with three different conditions: sealed with fibrin alone, sealed with fibrin plus autologous cells, and sealed with fibrin plus allogenic cells.
- Throughout the study (lasting twelve months), the horses were subjected to strenuous exercise. As part of the evaluation, various analyses were conducted, including assessment of lameness (an indirect measure of pain), radiographic imaging, gross inspection, as well as histological and immunohistochemical analyses.
Results Analysis
- The findings suggest that the use of autologous cells proved to be the most effective treatment. This was determined by observing that the repair tissue in defects treated with autologous cells had a significantly improved quality compared to those in the fibrin-only and control groups.
- Though allogenic cells did not prove superior to fibrin only treatment in most aspects, there was a greater percentage of type-II collagen in the repairs done using allogenic cells.
- Radiographic changes (which provide information about the bone structure below the cartilage) showed that the allogenic cell group had, on average, poorer results than the autologous cell group.
- Treatment with autologous cells significantly reduced the formation of central osteophytes (bony lumps formed as part of the body’s response to joint damage) compared to fibrin alone.
Conclusion
- The study concluded, based on scores from arthroscopy, radiography, and histology, that autologous cells embedded in fibrin offered better results than other treatments examined in the study.
- At this time, the use of allogenic cells cannot be recommended due to their inferior performance in promoting effective cartilage repair.
Cite This Article
APA
Frisbie DD, McCarthy HE, Archer CW, Barrett MF, McIlwraith CW.
(2015).
Evaluation of articular cartilage progenitor cells for the repair of articular defects in an equine model.
J Bone Joint Surg Am, 97(6), 484-493.
https://doi.org/10.2106/JBJS.N.00404 Publication
Researcher Affiliations
- Orthopaedic Research Center, Department of Clinical Sciences (D.D.F. and C.W.M.), and Department of Radiological and Health Sciences (M.F.B.), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523. E-mail address for D.D. Frisbie: david.frisbie@colostate.edu.
- Division of Pathophysiology and Repair, Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
- Institute of Life Sciences, Swansea University, Singleton Park, Swansea, SA2 8PP, United Kingdom.
- Orthopaedic Research Center, Department of Clinical Sciences (D.D.F. and C.W.M.), and Department of Radiological and Health Sciences (M.F.B.), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523. E-mail address for D.D. Frisbie: david.frisbie@colostate.edu.
- Orthopaedic Research Center, Department of Clinical Sciences (D.D.F. and C.W.M.), and Department of Radiological and Health Sciences (M.F.B.), College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523. E-mail address for D.D. Frisbie: david.frisbie@colostate.edu.
MeSH Terms
- Animals
- Arthroscopy
- Cartilage, Articular / injuries
- Cartilage, Articular / pathology
- Chondrocytes / transplantation
- Disease Models, Animal
- Femur
- Fibrin
- Horses
- Stem Cell Transplantation
- Transplantation, Autologous
- Transplantation, Homologous
- Wound Healing
Citations
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