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Home / Equine Research Bank / Osteoarthritis Cartilage / Evaluation of autologous chondrocyte transplantation via a c...
Osteoarthritis and cartilage2007; 16(6); 667-679; doi: 10.1016/j.joca.2007.09.013

Evaluation of autologous chondrocyte transplantation via a collagen membrane in equine articular defects: results at 12 and 18 months.

Abstract: To evaluate a technique of autologous chondrocyte implantation (ACI) similar to the other techniques using cell-seeded resorbable collagen membranes in large articular defects. Methods: Autologous cartilage was harvested arthroscopically from the lateral trochlear ridge of the femur in fifteen 3-year-old horses. After culture and expansion of chondrocytes the newly created ACI construct (autologous chondrocytes cultured expanded, seeded on a collagen membrane, porcine small intestine submucosa) was implanted into 15mm defects on the medial trochlear ridge of the femur in the opposite femoropatellar joint. Using two defects in each horse, the ACI technique was compared to collagen membrane alone (CMA) and empty cartilage defects (ECDs). Results: Arthroscopic evaluations at 4, 8, 12 and 18 months demonstrated that CMA was significantly worse compared to ACI or ECD treatments, with ACI having the best overall subjective grade. Overall raw histological scores demonstrated a significant improvement with ACI compared to either CMA or ECD treated defects and ACI defects had significantly more immunohistochemical staining for aggrecan than CMA or ECD treated defects (with significantly more type II collagen in ACI and ECD compared to CMA defects) at 12 and 18 months. Conclusions: Histologic and immunohistochemistry results from this long-term randomized study are particularly encouraging and demonstrate superiority with the ACI technique. Although there is no comparable study published with the traditional ACI technique in the horse (or with such a large defect size in another animal model), the use of a solid autologous cell-seeded-constructed implant would appear to offer considerable clinical advantages.
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Publication Date: 2007-11-26 PubMed ID: 18042409DOI: 10.1016/j.joca.2007.09.013Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research evaluates a technique called autologous chondrocyte implantation (ACI) that uses cell-seeded collagen membranes to treat large articular defects in horses. The study found this method to be superior, based on histological and immunohistochemistry results, compared to using just collagen membrane or leaving the defect empty.

Study Methodology

  • The study was conducted on fifteen 3-year-old horses.
  • Autologous cartilage was harvested arthroscopically from the lateral trochlear ridge of the femur, cultured, expanded, and seeded on a collagen membrane in a method known as the autologous chondrocyte implantation (ACI).
  • The new ACI construct was implanted into 15mm defects on the medial trochlear ridge of the femur in the opposite femoropatellar joint.
  • The technique was compared to defects treated with collagen membrane alone (CMA), and empty cartilage defects (ECDs).
  • The animals underwent arthroscopic evaluations at regular intervals (4, 8, 12, and 18 months) to assess the effects of the different treatments.

Study Findings

  • The study found that the defects treated with Collagen Membrane Alone (CMA) fared significantly worse than those treated with Autologous Chondrocyte Implantation (ACI) or Empty Cartilage Defects (ECDs).
  • The Autologous Chondrocyte Implantation (ACI) technique achieved the highest overall subjective grade.
  • Upon histological inspection, defects treated with ACI exhibited significant improvement compared to those treated with either CMA or ECD.
  • Defects treated with ACI showed significantly more immunohistochemical staining for aggrecan, a key component of cartilage, compared to CMA or ECD treated defects. ACI and ECD both had more type II collagen compared to CMA defects.

Conclusion

  • The results of this long-term randomized study indicate that the Autologous Chondrocyte Implantation (ACI) technique is superior in the treatment of large articular defects when compared with the usage of a collagen membrane alone (CMA) or leaving the defect empty (ECD).
  • There are no comparable studies using the traditional ACI technique on horses or such large defect sizes in other animals, making this study’s findings novel and important in the field.
  • The use of a solid autologous cell-seeded-constructed implant seems to have significant clinical advantages based on these findings.

Cite This Article

APA
Frisbie DD, Bowman SM, Colhoun HA, DiCarlo EF, Kawcak CE, McIlwraith CW. (2007). Evaluation of autologous chondrocyte transplantation via a collagen membrane in equine articular defects: results at 12 and 18 months. Osteoarthritis Cartilage, 16(6), 667-679. https://doi.org/10.1016/j.joca.2007.09.013

Publication

Osteoarthritis and cartilage
ISSN: 1063-4584
NlmUniqueID: 9305697
Country: England
Language: English
Volume: 16
Issue: 6
Pages: 667-679

Researcher Affiliations

Frisbie, D D
  • Orthopaedic Research Center (ORC), Colorado State University (CSU), Fort Collins, CO 80523, USA.
Bowman, S M
    Colhoun, H A
      DiCarlo, E F
        Kawcak, C E
          McIlwraith, C W

            MeSH Terms

            • Aggrecans / metabolism
            • Animals
            • Arthroscopy
            • Cartilage, Articular / injuries
            • Cartilage, Articular / metabolism
            • Cartilage, Articular / pathology
            • Cartilage, Articular / transplantation
            • Chondrocytes / metabolism
            • Chondrocytes / transplantation
            • Collagen
            • Collagen Type II / metabolism
            • Disease Models, Animal
            • Guided Tissue Regeneration / methods
            • Horses
            • Lameness, Animal / etiology
            • Stifle
            • Synovial Membrane / pathology
            • Tissue Engineering / methods
            • Treatment Outcome

            Citations

            This article has been cited 27 times.
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