Evaluation of automated methods of measuring hemoglobin and hematocrit in horses.
Abstract: To evaluate the accuracy of 3 automated methods of determining Hct and hemoglobin (Hb) concentration, compared with manual methods. Animals-22 clinically normal adult horses of various breeds. Methods: A blood sample was obtained from each horse. Six dilutions (representing Hct of 0, 10, 20, 40, 60, or 70%) were prepared from each sample and analyzed, using 1 of 2 blood gas analyzers or a hemoximeter (for automated determinations) or the Wintrobe macrohematocrit and cyanmethemoglobin methods (for manual determinations). Regression analysis was used to determine mean slope relationships between Hct and Hb measurements obtained by use of manual versus automated methods. Slopes were compared, using Student's t-test. Results: Of the 3 automated methods examined, only 1 blood gas analyzer reported Hct and Hb values that were not significantly different from those determined by use of manual methods; however, this analyzer could not report Hb concentrations below 2.5 g/dl. The other blood gas analyzer reported values for Hct and Hb concentrations that were consistently higher than those obtained by use of manual methods at Hct < or = 20% and Hb < or = 6.6 g/dl. The hemoximeter yielded more accurate results if the Hb concentration was between 6.6 and 20 g/dl. Conclusions: Although there were some limitations in measuring at low Hb concentrations, the method of determining Hb concentration and Hct with blood gas analyzer 2 was more accurate than that with blood gas analyzer 1 (Hct and Hb concentration) or the hemoximeter (Hb only).
Publication Date: 1998-12-19 PubMed ID: 9858398
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Adult Horses
- Analytical Methods
- Blood
- Clinical Examination
- Clinical Findings
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Equine Health
- Hematology
- Hemoglobin
- Horses
- Laboratory Methods
- Regression Analysis
- Veterinary Medicine
- Veterinary Practice
- Veterinary Procedure
- Veterinary Research
Summary
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This research article investigates the precision of three automated methods in determining Hematocrit (Hct) and Hemoglobin (Hb) concentrations in horses, using manual methodologies as a comparative benchmark.
Methodology
- The research involved analyzing blood samples obtained from 22 different clinically healthy adult horses of various breeds.
- Each sample was prepared into six different dilutions which represented Hematocrit of 0%,10%, 20%, 40%, 60%, or 70%.
- The dilutions were analyzed using either one of two blood gas analyzers or a hemoximeter for automated determinations. For manual determinations, the Wintrobe macrohematocrit and cyanmethemoglobin methods were used.
- Regression analysis was deployed to establish mean slope relationships between Hematocrit and Hemoglobin measurements using manual and automated methods.
- The differences in slopes obtained from the methods were examined using the Student’s t-test.
Results
- It was found that only one of the blood gas analyzers reported Hematocrit and Hemoglobin values that were not significantly distinct from those derived manually.
- However, the said blood gas analyzer could not deliver Hemoglobin concentrations below 2.5 g/dl.
- The other blood gas analyzer universally reported higher Hematocrit and Hemoglobin concentrations versus the manual methods when Hematocrit values were equal or less than 20% and Hemoglobin values were equal or less than 6.6 g/dl.
- The hemoximeter exhibited more accurate results when the Hemoglobin concentration fell in the range of 6.6 to 20 g/dl.
Conclusion
- The study concluded that while some limitations existed in measuring low Hemoglobin concentrations, Blood Gas Analyzer 2 provided more accurate determination of Hemoglobin concentration and Hematocrit than either Blood Gas Analyzer 1 or the Hemoximeter.
- The information derived from the study can help improve blood testing methods for horses and potentially for other animals or even humans.
Cite This Article
APA
Sorrell-Raschi LA, Tomasic M.
(1998).
Evaluation of automated methods of measuring hemoglobin and hematocrit in horses.
Am J Vet Res, 59(12), 1519-1522.
Publication
Researcher Affiliations
- Department of Clinical Studies (New Bolton Center) School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA.
MeSH Terms
- Animals
- Automation
- Blood Gas Analysis
- Blood Specimen Collection / methods
- Blood Specimen Collection / veterinary
- Hematocrit / methods
- Hematocrit / veterinary
- Hemoglobins / analysis
- Horses / blood
Citations
This article has been cited 1 times.- Prichard JS, French JS, Alvar N. Clinical evaluation of the ABL-77 for point-of-care analysis in the cardiovascular operating room. J Extra Corpor Technol 2006 Jun;38(2):128-33.
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