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Home / Equine Research Bank / Iran J Vet Res / Evaluation of cardiac troponin I, atrial natriuretic peptide...
Iranian journal of veterinary research2020; 21(3); 211-215;

Evaluation of cardiac troponin I, atrial natriuretic peptide and some oxidative/antioxidative biomarkers in the serum and hemolysate of trained Arabian horses after exercise.

Abstract: Limited information existed on performance tests in Iranian Arab horses. Objective: The objective of this study is to investigate time related changes of cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and oxidative/antioxidant biomarkers in the serum of Arabian horses before and after regular training. Methods: Blood samples were collected from jugular vein of 25 Arabian horses before exercise; 5 h and 18 h after exercise and used to measure the cTnI, ANP, malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), glutathione peroxidase (GPX), and superoxide dismutase (SOD) concentrations. Data analysis was performed using SAS. Results: Significant time related changes were seen for cTnI, MDA, and GPX concentrations (P<0.05). There were no time significant variations in the concentrations of ANP, FRAP and SOD. The values of cTnI and MDA significantly increased after exercise. The amounts of GPX significantly increased 5 h after exercise and then decreased up to 18 h after exercise. Conclusions: The results of the present study can be used in future studies in evaluating the health status of Arabian horses. In addition, the present results can be used as primarily described data in the evaluation of Arabian horses.
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Publication Date: 2020-11-13 PubMed ID: 33178299PubMed Central: PMC7608043
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study examined the changes in certain biomarkers in Arabian horses following exercise to gather performance data and understand the health status of the horses. Specifically, the study focused on cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and oxidative/antioxidant biomarkers.

Research Objective

  • The purpose of the study was to investigate how the levels of certain biomarkers – cTnI, ANP, and oxidative/antioxidant biomarkers – in the serum of Arabian horses change over time after regular training.
  • Among the targeted biomarkers were malondialdehyde (MDA), a marker of oxidative stress; ferric reducing antioxidant power (FRAP), an indicator of antioxidant activity; and two antioxidant enzymes, glutathione peroxidase (GPX), and superoxide dismutase (SOD).

Methods Used

  • Blood samples were taken from the jugular vein of 25 Arabian horses before exercise, 5 hours after exercise, and 18 hours after exercise. These samples were used to measure the concentrations of the targeted biomarkers.
  • Data analysis was carried out using the Statistical Analysis System (SAS).

Results of the Study

  • Significantly time-related changes were observed in cTnI, MDA, and GPX concentrations, indicating that exercise impacted these specific biomarkers.
  • The levels of cTnI and MDA increased following exercise, suggesting an increase in heart strain and oxidative stress, respectively.
  • The GPX concentration reportedly increased 5 hours post-exercise, and then decreased up to 18 hours after exercise. This pattern points to the body’s antioxidant response post-exercise.
  • However, there weren’t any time-based significant changes in the concentrations of ANP, FRAP and SOD observed over the course of the study, indicating these parameters may not be sensitive to physical stress in Arabian horses.

Conclusions

  • The outcomes of the study can be used in future research for evaluating the health status of Arabian horses and also serve as a performance index.
  • The findings can also be used as preliminary data in the veterinary assessment of Arabian horses, supporting the potential for early detection of exercise-induced health issues.

Cite This Article

APA
Pourmohammad R, Mohri M, Seifi HA, Sardari K. (2020). Evaluation of cardiac troponin I, atrial natriuretic peptide and some oxidative/antioxidative biomarkers in the serum and hemolysate of trained Arabian horses after exercise. Iran J Vet Res, 21(3), 211-215.

Publication

Iranian journal of veterinary research
ISSN: 1728-1997
NlmUniqueID: 101660030
Country: Iran
Language: English
Volume: 21
Issue: 3
Pages: 211-215

Researcher Affiliations

Pourmohammad, R
  • Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Mohri, M
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Seifi, H A
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Sardari, K
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

References

This article includes 24 references
  1. Andriichuk A, Tkachenko H. Effect of gender and exercise on haematological and biochemical parameters in Holsteiner horses.. J Anim Physiol Anim Nutr (Berl) 2017 Oct;101(5):e404-e413.
    pubmed: 28066938doi: 10.1111/jpn.12620google scholar: lookup
  2. Andriichuk A, Tkachenko H, Kurhaluk N. Gender differences of oxidative stress biomarkers and erythrocyte damage in well-trained horses during exercise.. J. Equine Vet. Sci. 2014;185:172–175.
  3. Andriichuk A, Tkachenko H, Tkachova I. Oxidative stress biomarkers and erythrocytes hemolysis in well-trained equine athletes before and after exercise.. J. Equine Vet. Sci. 2016;36:32–43.
  4. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.. Anal Biochem 1996 Jul 15;239(1):70-6.
    pubmed: 8660627doi: 10.1006/abio.1996.0292google scholar: lookup
  5. Durando MM, Reef VB, Kline K, Birks EK. Acute effects of short duration maximal exercise on cardiac troponin I in healthy horses.. Equine Comp. Exerc. Physiol. 2006;3:217–223.
  6. Fazio F, Cecchini S, Saoca C, Caputo AR, Lancellotti M, Piccione G. Relationship of some oxidative stress biomarkers in jumper horses after regular training program.. J. Equine Vet. Sci. 2016;47:20–24.
  7. Ginsburg GS, O'Toole M, Rimm E, Douglas PS, Rifai N. Gender differences in exercise-induced changes in sex hormone levels and lipid peroxidation in athletes participating in the Hawaii Ironman triathlon. Ginsburg-gender and exercise-induced lipid peroxidation.. Clin Chim Acta 2001 Mar;305(1-2):131-9.
    pubmed: 11249932doi: 10.1016/s0009-8981(00)00427-7google scholar: lookup
  8. Gondim FJ, Zoppi CC, Silveria L, Pereira-Da-Silva L, Vaz-De-Macedo D. Possible relationship between performance and oxidative stress in endurance horses.. J. Equine Vet. Sci. 2009;29:206–212.
  9. Ilhan N, Kamanli A, Ozmerdivenli R, Ilhan N. Variable effects of exercise intensity on reduced glutathione, thiobarbituric acid reactive substance levels, and glucose concentration.. Arch Med Res 2004 Jul-Aug;35(4):294-300.
    pubmed: 15325503doi: 10.1016/j.arcmed.2004.03.006google scholar: lookup
  10. Kirschvink N, de Moffarts B, Farnir F, Pincemail J, Lekeux P. Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds.. Equine Vet J Suppl 2006 Aug;(36):239-44.
    pubmed: 17402425doi: 10.1111/j.2042-3306.2006.tb05546.xgoogle scholar: lookup
  11. Kraus MS, Jesty SA, Gelzer AR, Ducharme NG, Mohammed HO, Mitchell LM, Soderholm LV, Divers TJ. Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease.. Am J Vet Res 2010 Jan;71(1):55-9.
    pubmed: 20043781doi: 10.2460/ajvr.71.1.55google scholar: lookup
  12. Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses.. Oxid Med Cell Longev 2012;2012:920932.
    pmc: PMC3423952pubmed: 22919442doi: 10.1155/2012/920932google scholar: lookup
  13. Lippi G, Schena F, Salvagno GL, Montagnana M, Gelati M, Tarperi C, Banfi G, Guidi GC. Influence of a half-marathon run on NT-proBNP and troponin T.. Clin Lab 2008;54(7-8):251-4.
    pubmed: 18942493
  14. Muñoz-Escassi B, Maranon GA, Manley W, Riber CC, Cayado P, Leon R, Garcia C, Vara EMG. Exercise-induced changes on lipid peroxides and antioxidant enzymes levels changes in plasma of show jumping and dressage horses.. Int. J. Appl. Res. Vet. M. 2006;4:274–281.
  15. Nostell K, Häggström J. Resting concentrations of cardiac troponin I in fit horses and effect of racing.. J Vet Cardiol 2008 Dec;10(2):105-9.
    pubmed: 19019757doi: 10.1016/j.jvc.2008.10.001google scholar: lookup
  16. Petibois C, Déléris G. Erythrocyte adaptation to oxidative stress in endurance training.. Arch Med Res 2005 Sep-Oct;36(5):524-31.
    pubmed: 16099333doi: 10.1016/j.arcmed.2005.03.047google scholar: lookup
  17. Ruskoaho H. Cardiac hormones as diagnostic tools in heart failure.. Endocr Rev 2003 Jun;24(3):341-56.
    pubmed: 12788803doi: 10.1210/er.2003-0006google scholar: lookup
  18. Shave R, Oxborough D. Exercise-induced cardiac injury: evidence from novel imaging techniques and highly sensitive cardiac troponin assays.. Prog Cardiovasc Dis 2012 Mar-Apr;54(5):407-15.
    pubmed: 22386291doi: 10.1016/j.pcad.2012.01.007google scholar: lookup
  19. Smarsh DN, Williams CA. Oxidative stress and antioxidant status in standardbreds: effect of age and training in resting plasma and muscle.. J. Equine Vet. Sci. 2017;48:93–102.
  20. Soares JCM, Mandes JC, Zanella R, Bondan C, Alves LP, De-Lima MR, Da-Motta AC, Zanella EL. Biochemical and antioxidant changes in plasma, serum, and erythrocytes of horses before and after a jumping competition.. J. Equine Vet. Sci. 2011;37:357–360.
  21. Trachsel DS, Grenacher B, Weishaupt MA, Schwarzwald CC. Plasma atrial natriuretic peptide concentrations in horses with heart disease: a pilot study.. Vet J 2012 May;192(2):166-70.
    pubmed: 21782487doi: 10.1016/j.tvjl.2011.06.035google scholar: lookup
  22. Trachsel DS, Schwarzwald CC, Bitschnau C, Grenacher B, Weishaupt MA. Atrial natriuretic peptide and cardiac troponin I concentrations in healthy Warmblood horses and in Warmblood horses with mitral regurgitation at rest and after exercise.. J Vet Cardiol 2013 Jun;15(2):105-21.
    pubmed: 23684505doi: 10.1016/j.jvc.2012.12.003google scholar: lookup
  23. Van Der Vekens N, Decloedt A, Clereq DD, Verheyen T, Van-Loon G. The use of cardiac biomarkers in veterinary medicine: The equine perspective.. Flem. Vet. J. 2012;81:319–327.
  24. Walton, RM . Equine clinical pathology. 1st Edn. Ames, Iowa, USA, John Wiley and Sons Inc; 2014. pp. 160–161.

Citations

This article has been cited 1 times.
  1. Hanås S, Larsson A, Rydén J, Lilliehöök I, Häggström J, Tidholm A, Höglund K, Ljungvall I, Holst BS. Cardiac troponin I in healthy Norwegian Forest Cat, Birman and domestic shorthair cats, and in cats with hypertrophic cardiomyopathy.. J Feline Med Surg 2022 Oct;24(10):e370-e379.
    doi: 10.1177/1098612X221117115pubmed: 36073987google scholar: lookup
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