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Journal of equine veterinary science2021; 104; 103687; doi: 10.1016/j.jevs.2021.103687

Evaluation of Different Blood Parameters From Endurance Horses Competing at 160 km.

Abstract: The purpose of this study was to assess a change in different blood parameters before and after a 160 km endurance race and to evaluate differences in cardiac biomarkers between horses that completed the race and horses that did not. The study population consisted of 52 healthy endurance horses. Horses participating in the study were assigned to three groups: horses that successfully completed the race ("finishers"), horses that failed to qualify at the veterinary check for primarily metabolic reasons ("metabolic") and horses that failed to qualify at the veterinary check for primarily gait related reasons ("gait related"). The latter two groups were combined to form a final group of "non-finishers" that were excluded for either "gait related" or "metabolic" disorders. Venous blood samples were taken before and after the endurance race. Serum and EDTA-plasma were analyzed for cardiac troponin I (cTNI), heart fatty acid binding protein (HFABP), alpha-hydroxybutyrate dehydrogenase (α-HBDH), atrial natriuretic peptide (ANP), lactate dehydrogenase (LDH), symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA). Lactate dehydrogenase (P = .001), SDMA (P= .001) and ADMA (P= .002) increased significantly after the endurance race in the finisher group. A significant increase in cTNI and α-HBDH concentration after the endurance race compared to the values before the endurance race was detected in the finisher (P= .001, P= .001) and gait related group (P= .002, P= .007). The longer the distance completed, the more these five blood parameters increased. No differences between the groups could be found and none of the measured blood parameters showed significant differences among groups before or after racing.
Publication Date: 2021-06-19 PubMed ID: 34416987DOI: 10.1016/j.jevs.2021.103687Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research article investigates how different blood parameters change in endurance horses before and after a 160 km race. The study also examined the differences in cardiac biomarkers between the horses that finished the race and those that did not.

Study Design and Sample Population

  • The research involved 52 healthy endurance horses that were classified into three groups. These included horses that successfully completed the race (finishers), those that failed the veterinary check primarily due to metabolic reasons (metabolic), and those that did not pass the veterinary check due to primarily gait-related issues (gait-related).
  • The metabolic and gait-related group were further combined to form the ‘non-finishers’ group.

Methodology

  • The researchers collected venous blood samples from the horses before and after the race. They examined these samples for various parameters, including cardiac troponin I (cTNI), α-HBDH, HFABP, ANP, LDH, symmetric dimethylarginine (SDMA), and asymmetric dimethylarginine (ADMA).
  • These blood parameters were chosen because of their relevance to cardiovascular health and exercise physiology.

Findings

  • The study found that LDH, SDMA, and ADMA increased significantly in the finisher group following the race.
  • There was a significant increase in cTNI and α-HBDH concentration in both the finisher and gait-related groups after the race compared to the values before the endurance race.
  • Furthermore, the study revealed that the longer the distance completed by the horses, the more these five blood parameters increased.
  • Despite these findings, there were no significant differences between the finisher and non-finisher groups in the measured blood parameters either before or after the race.

Conclusions and Implications

  • From the results obtained, one can conclude that endurance racing can influence certain blood parameters in horses. The increase in these parameters suggests that prolonged athletic activity may strain the cardiovascular system and affect its function over time.
  • However, the absence of differences between finishers and non-finishers in regard to these cardiac biomarkers suggests that there may not be clear biomarkers to predict race performance.

Cite This Article

APA
Ertelt A, Merle R, Stumpff F, Bollinger L, Liertz S, Weber C, Gehlen H. (2021). Evaluation of Different Blood Parameters From Endurance Horses Competing at 160 km. J Equine Vet Sci, 104, 103687. https://doi.org/10.1016/j.jevs.2021.103687

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 104
Pages: 103687
PII: S0737-0806(21)00317-8

Researcher Affiliations

Ertelt, Antonia
  • Equine Clinic, Internal Medicine, Freie Universitaet Berlin, Berlin, Germany. Electronic address: antonia.ertelt@fu-berlin.de.
Merle, Roswitha
  • Institute for Veterinary Epidemiology and Biostatistics, Freie Universitaet Berlin, Berlin, Germany.
Stumpff, Friederike
  • Institute of Veterinary Physiology, Freie Universitaet Berlin, Berlin, Germany.
Bollinger, Lena
  • Equine Clinic, Internal Medicine, Freie Universitaet Berlin, Berlin, Germany.
Liertz, Sarah
  • Equine Clinic, Internal Medicine, Freie Universitaet Berlin, Berlin, Germany.
Weber, Corinna
  • Laboklin Veterinary Diagnostic Laboratory, Bad Kissingen, Germany.
Gehlen, Heidrun
  • Equine Clinic, Internal Medicine, Freie Universitaet Berlin, Berlin, Germany.

MeSH Terms

  • Animals
  • Gait
  • Horses
  • L-Lactate Dehydrogenase
  • Physical Conditioning, Animal
  • Physical Endurance
  • Troponin I

Citations

This article has been cited 6 times.
  1. Galen GV, Olsen E, Siwinska N. Biomarkers of Kidney Disease in Horses: A Review of the Current Literature. Animals (Basel) 2022 Oct 5;12(19).
    doi: 10.3390/ani12192678pubmed: 36230418google scholar: lookup
  2. Arfuso F, Rizzo M, Giannetto C, Giudice E, Cirincione R, Cassata G, Cicero L, Piccione G. Oxidant and Antioxidant Parameters' Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise. Antioxidants (Basel) 2022 Jun 15;11(6).
    doi: 10.3390/antiox11061176pubmed: 35740073google scholar: lookup
  3. Kirsch K, Fercher C, Horstmann S, von Reitzenstein C, Augustin J, Lagershausen H. Monitoring Performance in Show Jumping Horses: Validity of Non-specific and Discipline-specific Field Exercise Tests for a Practicable Assessment of Aerobic Performance. Front Physiol 2021;12:818381.
    doi: 10.3389/fphys.2021.818381pubmed: 35095574google scholar: lookup
  4. Čebulj-Kadunc N, Frangež R, Kruljc P. Long-Term Changes of Physiological Reactions in Young Lipizzan Stallions During Exercise Testing. Animals (Basel) 2025 Aug 23;15(17).
    doi: 10.3390/ani15172479pubmed: 40941274google scholar: lookup
  5. Foreman JH, Tennent-Brown BS, Oyama MA, Sisson DD. Plasma Cardiac Troponin-I Concentration in Normal Horses and in Horses with Cardiac Abnormalities. Animals (Basel) 2025 Jan 3;15(1).
    doi: 10.3390/ani15010092pubmed: 39795035google scholar: lookup
  6. Giers J, Bartel A, Kirsch K, Müller SF, Horstmann S, Gehlen H. Blood-Based Markers for Skeletal and Cardiac Muscle Function in Eventing Horses before and after Cross-Country Rides and How They Are Influenced by Plasma Volume Shift. Animals (Basel) 2023 Oct 5;13(19).
    doi: 10.3390/ani13193110pubmed: 37835716google scholar: lookup