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Clinical and experimental immunology1979; 38(1); 37-44;

Evaluation of functional thymic hormones in Arabian horses with severe combined immunodeficiency.

Abstract: Arabian horses with severe combined immunodeficiency disease (SCID) were evaluated for thymic hormone activities using thymic extracts and sera. Extracts prepared from thymus of SCID horses were able to increase the number of spleen cells responding to sheep red blood cells in irradiated, bone marrow-reconstituted mice. In addition, ultrafiltrates prepared from sera of these immunodeficient horses, which contained material with molecular weight of less than 50,000 Daltons could (a) induce a population of human bone marrow precursor cells to differentiate into cells bearing SRBC receptors and form spontaneous E-rosettes, a characteristic of T lymphocytes, and (b) stimulate cyclic adenosine monophosphate (AMP) synthesis in mouse thymocytes. Based on in vivo and in vitro effects, it was concluded that the defect of these Arabian horses with severe combined immunodeficiency disease did not involve a complete thymic hormone inadequacy.
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Publication Date: 1979-10-01 PubMed ID: 230925PubMed Central: PMC1537827
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  • Journal Article
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates whether Arabian horses with severe combined immunodeficiency (SCID), a serious disease that impairs the immune system, have adequate hormone activity in their thymus. The results suggest that SCID in these horses does not involve a complete deficiency of thymic hormones.

Introduction

This study focuses on Arabian horses affected by severe combined immunodeficiency disease (SCID), a genetic disorder that dramatically weakens the immune system, making the animal susceptible to infections. The researchers aim to examine the functionality of the hormones from the thymus, an organ critical for proper immune function, in these SCID-affected horses.

Research Method

  • The study relies on the use of extracts from the thymus gland and serum (a component of blood) taken from SCID-affected horses.
  • The team prepared ultrafiltrates from serum samples; these ultrafiltrates have a molecular weight of less than 50,000 Daltons, allowing them to be used in subsequent evaluations of hormone activities.

Testing Thymic Hormone Activities

  • The thymic extracts from SCID horses were administered to irradiated, bone marrow-reconstituted mice to increase the response of their spleen cells to sheep red blood cells. This process illustrates the influence of the thymic hormones on the immune response mechanism.
  • The serum ultrafiltrates were used to stimulate a subpopulation of human bone marrow precursor cells. The stimulated cells exhibited characteristics of T lymphocytes, indicating that the ultrafiltrate has the ability to influence the differentiation of these immune cells.
  • The ultrafiltrate also showed its capacity to stimulate the production of cyclic adenosine monophosphate (AMP) in mouse thymocytes (immature T cells), indicating hormone activity related to cell functionality and signaling.

Conclusions

  • Through both in vivo and in vitro testing, the study concludes that Arabian horses with SCID do not experience a total inadequacy in thymic hormone activities.
  • This insight significantly aids in understanding the pathology of SCID in horses and provides a basis for potential treatment approaches, which might focus on optimizing hormone functionality rather than treating a total hormone deficit.

Cite This Article

APA
Splitter GA, Incefy G, Iwata T, McGuire TC. (1979). Evaluation of functional thymic hormones in Arabian horses with severe combined immunodeficiency. Clin Exp Immunol, 38(1), 37-44.

Publication

Clinical and experimental immunology
ISSN: 0009-9104
NlmUniqueID: 0057202
Country: England
Language: English
Volume: 38
Issue: 1
Pages: 37-44

Researcher Affiliations

Splitter, G A
    Incefy, G
      Iwata, T
        McGuire, T C

          MeSH Terms

          • Aging
          • Animals
          • Binding Sites
          • Cattle
          • Cyclic AMP / metabolism
          • Erythrocytes / immunology
          • Hemolytic Plaque Technique
          • Horses
          • Humans
          • Immunologic Deficiency Syndromes / immunology
          • Mice
          • Rosette Formation
          • Sheep
          • T-Lymphocytes / metabolism
          • Thymic Factor, Circulating / metabolism
          • Thymus Gland / physiopathology
          • Thymus Hormones / metabolism

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          Citations

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