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Home / Equine Research Bank / Anim Reprod Sci / Evaluation of genes involved in prostaglandin action in equi...
Animal reproduction science2010; 122(1-2); 124-132; doi: 10.1016/j.anireprosci.2010.08.007

Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy.

Abstract: The aim was to evaluate expression of genes involved in the biosynthesis of prostaglandins (PTG), Prostaglandin H Synthase-1 (PTGS1) and PTGS2, PGF synthase (PTGFS), and PGE synthase (PTGES), PGF receptor (PTGFR), PGE receptors (PTGER2 and PTGER4), prostaglandin transporter (SLCO2A1) and hydroxyprostaglandin dehydrogenase-15 (HPGD). Endometrial biopsies were obtained from mares on day of ovulation (d0, n=4), late diestrus (LD, n=4), early luteolysis (EL, n=4) and after luteolysis (AL, n=4) during the cycle. Stages of the cycle were confirmed by plasma progesterone concentrations measured daily and ultrasound examinations. Biopsies were also taken on days 14 (P14; n=4), 15 (P15, n=4), 18 (P18, n=4) and 22 (P22; n=4) of pregnancy. Relative mRNA expressions were quantified using real-time RT-PCR. A mixed model was fitted on the normalized data and least significant difference test (α=0.05) was employed. Expression of PTGS1 mRNA was low throughout the estrous cycle and early days of pregnancy, but upregulated on P18 and P22. PTGS2 expression was increased on EL, but it was suppressed by pregnancy on P15, P18, and P22. PTGFS expression was upregulated in both cyclic and pregnant mares compared to d0 and its level was the highest on LD. PTGFR expression was transiently increased on LD and EL and was suppressed during early pregnancy. Both PTGES and PTGER2 expressions were increased on LD, EL, and early pregnancy, but were decreased after the luteolysis in cyclic mares as they remained high on P18 and P22. PTGER4 expression did not change throughout the cycle and early pregnancy. Levels of HPGD and SLCO2A1 were significantly increased only on P22. In conclusion, PTGS2 expression increases around the time of luteolysis and concurrent upregulation of PTGFS and PTGES indicates that equine endometrium has increased capability of PTG production around the time of luteolysis. However, pregnancy reduces PTGS2 expression, but maintains the high levels of PTGES during early pregnancy along with PTGER2 while PTGFR expression was suppressed. These findings suggest that possible luteotrophic action of PGE₂ is required in early equine pregnancy. PTGS1 is only upregulated later in the early pregnancy suggesting that it is not involved in luteolysis, but could be the main PTGS enzyme at this time during early pregnancy. An increase in HPGD and SLCO2A1 levels on P22 indicates a tight regulation of PTG action by pregnancy.
Copyright © 2010 Elsevier B.V. All rights reserved.
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Publication Date: 2010-08-13 PubMed ID: 20832957DOI: 10.1016/j.anireprosci.2010.08.007Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on investigating the expression of genes related to the production and function of prostaglandins (PTGs) in the endometrium of mares during their estrous cycle and the early phase of pregnancy. It notes changes in PTGS1, PTGS2, PTGFS, PTGES, PTGFR, PTGER2, PTGER4, SLCO2A1, and HPGD expression levels during different stages of the estrous cycle and pregnancy.

Research Methods and Experimental Procedures

  • The researchers extracted endometrial tissues from mares during various stages of their reproductive cycle and early pregnancy.
  • Stages of the reproductive cycle were confirmed by measuring daily plasma progesterone concentrations and ultrasound examinations.
  • The mRNA expressions of the different genes were quantified using real-time reverse transcription PCR (RT-PCR).
  • To find meaningful results, a statistical model was applied to the normalized data.

Findings of the Research

  • PTGS1 mRNA expression was low during the estrous cycle and early pregnancy, but increased on the 18th and 22nd days of pregnancy.
  • PTGS2 expression increased during early luteolysis (decline in the corpus luteum after ovulation) but was reduced during pregnancy on the 15th, 18th, and 22nd days of the reproductive cycle.
  • Expression of PTGFS (responsible for prostaglandin synthesis) was high during both cyclic and pregnancy stages compared to the day of ovulation.
  • Expresssion of PTGFR (prostaglandin receptor) was temporarily increased during late diestrus and early luteolysis stages, but was reduced during early pregnancy.
  • Both PTGES and PTGER2 expressions increased during late diestrus, early luteolysis, and early pregnancy, but were decreased after luteolysis during the cycle period as they remained high on the 18th and 22nd days of the pregnancy.
  • PTGER4 expression levels remained constant throughout the entire cycle and during early pregnancy.
  • Levels of HPGD and SLCO2A1 significantly increased only on the 22nd day of pregnancy.

Conclusion and Interpretation

  • PTGS2 expression increases during luteolysis suggesting an increased capability for the equine endometrium to produce PTGs at this time.
  • Early pregnancy reduces PTGS2 expression but maintains high levels of PTGES along with PTGER2, suggesting that PGE₂ luteotrophic action may be required early in equine pregnancy.
  • PTGS1 is not associated with luteolysis, but is upregulated later during early pregnancy indicating that it may be the main PTGS enzyme present during this time.
  • The increase in HPGD and SLCO2A1 levels on day 22 suggests a tight control of PTG function by pregnancy.

Cite This Article

APA
Atli MO, Kurar E, Kayis SA, Aslan S, Semacan A, Celik S, Guzeloglu A. (2010). Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy. Anim Reprod Sci, 122(1-2), 124-132. https://doi.org/10.1016/j.anireprosci.2010.08.007

Publication

Animal reproduction science
ISSN: 1873-2232
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 122
Issue: 1-2
Pages: 124-132

Researcher Affiliations

Atli, Mehmet O
  • Department of OBGYN, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
Kurar, Ercan
    Kayis, Seyit A
      Aslan, Selim
        Semacan, Ahmet
          Celik, Sefa
            Guzeloglu, Aydin

              MeSH Terms

              • Animals
              • Endometrium / diagnostic imaging
              • Endometrium / metabolism
              • Estrous Cycle / genetics
              • Female
              • Gene Expression Regulation, Developmental
              • Horses / metabolism
              • Hydroxyprostaglandin Dehydrogenases / genetics
              • Luteolysis / genetics
              • Luteolysis / metabolism
              • Organic Anion Transporters / genetics
              • Pregnancy
              • Pregnancy, Animal / genetics
              • Progesterone / blood
              • Prostaglandin-Endoperoxide Synthases / genetics
              • Prostaglandins / genetics
              • Receptors, Prostaglandin / genetics
              • Ultrasonography

              Citations

              This article has been cited 6 times.
              1. Rudolf Vegas A, Podico G, Canisso IF, Bollwein H, Almiñana C, Bauersachs S. Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare.. Sci Rep 2021 Nov 16;11(1):22293.
                doi: 10.1038/s41598-021-01785-3pubmed: 34785745google scholar: lookup
              2. Klein C, Bruce P, Hammermueller J, Hayes T, Lillie B, Betteridge K. Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares.. PLoS One 2021;16(10):e0257161.
                doi: 10.1371/journal.pone.0257161pubmed: 34614002google scholar: lookup
              3. Ibrahim S, Hedia M, Taqi MO, Derbala MK, Mahmoud KGM, Ahmed Y, Ismail S, El-Belely M. Alterations in the Expression Profile of Serum miR-155, miR-223, miR-17, miR-200a, miR-205, as well as Levels of Interleukin 6, and Prostaglandins during Endometritis in Arabian Mares.. Vet Sci 2021 Jun 4;8(6).
                doi: 10.3390/vetsci8060098pubmed: 34199703google scholar: lookup
              4. Srikanth K, Park W, Lim D, Lee KT, Jang GW, Choi BH, Ka H, Park JE, Kim JM. Serial gene co-expression network approach to mine biological meanings from integrated transcriptomes of the porcine endometrium during estrous cycle.. Funct Integr Genomics 2020 Jan;20(1):117-131.
                doi: 10.1007/s10142-019-00703-1pubmed: 31396752google scholar: lookup
              5. Dik B, Sonmez G, Faki HE, Bahcivan E. Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats.. Exp Anim 2018 Nov 1;67(4):403-412.
                doi: 10.1538/expanim.18-0029pubmed: 29731490google scholar: lookup
              6. Griffith OW, Chavan AR, Protopapas S, Maziarz J, Romero R, Wagner GP. Embryo implantation evolved from an ancestral inflammatory attachment reaction.. Proc Natl Acad Sci U S A 2017 Aug 8;114(32):E6566-E6575.
                doi: 10.1073/pnas.1701129114pubmed: 28747528google scholar: lookup
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