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Home / Equine Research Bank / Vet Parasitol / Evaluation of in vitro and in vivo inhibitory effects of fus...
Veterinary parasitology2012; 191(1-2); 1-10; doi: 10.1016/j.vetpar.2012.08.022

Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and Theileria parasites.

Abstract: Fusidic acid known to has antibacterial, antifungal, and antimalarial activities. Fusidic acid blocks translation elongation factor G gene in Plasmodium falciparum. In the present study, the inhibitory effects of fusidic acid on the in vitro growth of bovine and equine Babesia parasites were evaluated. The inhibitory effect of fusidic acid on the in vivo growth of Babesia microti was also assessed. The in vitro growth of four Babesia species that were tested was significantly inhibited (P<0.05) by micromolar concentrations of fusidic acid (IC(50) values=144.8, 17.3, 33.3, and 56.25 μM for Babesia bovis, Babesia bigemina, Babesia caballi, and Theileria equi, respectively). Combinations of fusidic acid with diminazene aceturate synergistically potentiated its inhibitory effects in vitro on B. bovis and B. caballi. In B. microti-infected mice, fusidic acid caused significant (P<0.05) inhibition of the growth of B. microti at the dose of 500 mg/kg BW relative to control group. These results indicate that fusidic acid might be incorporated in treatment of babesiosis.
Copyright © 2012 Elsevier B.V. All rights reserved.
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Publication Date: 2012-09-04 PubMed ID: 22985928DOI: 10.1016/j.vetpar.2012.08.022Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article presents a study on the potential use of fusidic acid as a treatment for infections caused by Babesia and Theileria parasites. It was found that fusidic acid significantly inhibited the growth of these parasitic species both in vitro and in vivo.

Study Overview

  • The study investigated the inhibitory effects of fusidic acid on the growth of Babesia and Theileria parasites. These parasites are known to cause babesiosis, a malaria-like illness in animals and occasionally in humans.
  • Fusidic acid, a compound with established antibacterial, antifungal, and antimalarial properties, was tested against four different Babesia species in vitro (in a lab setting rather than in a living organism).

Study Methods

  • Researchers used different concentrations of fusidic acid and evaluated its inhibitory effects on the growth of the parasites. The aim was to determine the IC(50) values, which indicate the concentration of a substance needed to inhibit the growth of a microorganism by 50%.
  • Additionally, the researchers investigated the potential synergistic effects of combining fusidic acid with diminazene aceturate, a common drug used to treat babesiosis.
  • To study the in vivo effects of fusidic acid, the researchers infected mice with Babesia microti and then treated them with the compound.

Study Findings

  • The study revealed that fusidic acid significantly inhibited the in vitro growth of all four tested Babesia species. Specifically, the compound had IC(50) values ranging from 17.3 to 144.8 µM for the different species.
  • The researchers also discovered that a combination of fusidic acid with diminazene aceturate potentiated the inhibitory effects of fusidic acid on B. bovis and B. caballi in vitro.
  • The in vivo tests, involving B. microti-infected mice, showed that fusidic acid significantly inhibited the growth of the parasites at a dosage of 500 mg/kg body weight. This measurement was relative to a control group.

Conclusion

  • Based on these findings, the researchers suggested that fusidic acid might have potential for incorporation into treatment strategies for babesiosis. However, they did not elaborate on the potential side effects or safety profile of fusidic acid at the evaluated dosages, indicating that further research would be needed.

Cite This Article

APA
Salama AA, Aboulaila M, Moussa AA, Nayel MA, El-Sify A, Terkawi MA, Hassan HY, Yokoyama N, Igarashi I. (2012). Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and Theileria parasites. Vet Parasitol, 191(1-2), 1-10. https://doi.org/10.1016/j.vetpar.2012.08.022

Publication

Veterinary parasitology
ISSN: 1873-2550
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 191
Issue: 1-2
Pages: 1-10
PII: S0304-4017(12)00435-9

Researcher Affiliations

Salama, Akram Ahmed
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan.
Aboulaila, Mahmoud
    Moussa, Ahmed A
      Nayel, Mohamed A
        El-Sify, Ahmed
          Terkawi, Mohamad A
            Hassan, Hany Y
              Yokoyama, Naoaki
                Igarashi, Ikuo

                  MeSH Terms

                  • Animals
                  • Antiparasitic Agents / pharmacology
                  • Antiparasitic Agents / therapeutic use
                  • Babesia / drug effects
                  • Babesiosis / drug therapy
                  • Cells, Cultured
                  • Diminazene / analogs & derivatives
                  • Diminazene / pharmacology
                  • Diminazene / therapeutic use
                  • Drug Synergism
                  • Female
                  • Fusidic Acid / pharmacology
                  • Fusidic Acid / therapeutic use
                  • Inhibitory Concentration 50
                  • Mice
                  • Mice, Inbred BALB C
                  • Theileria / drug effects
                  • Theileriasis / drug therapy
                  • Time Factors

                  Citations

                  This article has been cited 17 times.
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