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Journal of veterinary science2019; 20(6); e60; doi: 10.4142/jvs.2019.20.e60

Evaluation of multiple allergen simultaneous (sIgE) testing compared to intradermal testing in the etiological diagnosis of atopic dermatitis in horses.

Abstract: Although intradermal testing (IDT) is commonly used in the etiological diagnosis of allergies, in vitro testing for specific IgE (sIgE) is an attractive alternative. Currently, new laboratory techniques in veterinary allergological practice, including multiple allergen simultaneous tests (MASTs), gradually supersede in vivo tests. Both, serological (sIgE) and IDTs in fourteen atopic Malopolski horses were performed. Correlation and agreement between test results were evaluated. Receiver operating characteristic analysis showed that sIgE to had the best diagnostic performance (Area under the ROC curve [AUC] = 0.969), followed by (AUC = 0.844), (AUC = 0.813) and (AUC = 0.803). A significant positive correlation between IDT and MAST was found for (r = 0.870; = 0.00005), and (r = 0.657; = 0.011). There was significant moderate agreement for 2 of 5 allergens, (κ = 0.569) and (κ = 0.485) in semiquantitative assessment and significant fair to substantial agreement for 3 of 5 allergens, (κ = 0.689), (κ = 0.569), (κ = 0.432) in dichotomic assessment. Sensitivity ranged from 44% to 89%, depending on the allergen, while specificity was significantly higher for all allergens in MAST (60%-100%); the mean accuracy was 73% (manufacturer cut-off) and 77.4% (optimal cut-off) based on the Youden index. Compared with IDT, serological MAST showed good detection performance for 60% allergen sIgE in dichotomic assessment with substantial diagnostic capability, but careful clinical interpretation is needed for some allergens.
© 2019 The Korean Society of Veterinary Science.
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Publication Date: 2019-11-28 PubMed ID: 31775187PubMed Central: PMC6883192DOI: 10.4142/jvs.2019.20.e60Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the effectiveness of intradermal testing (IDT) versus in vitro specific IgE (sIgE) testing, specifically using multiple allergen simultaneous tests (MASTs) for detecting allergies in Malopolski horses. The study found substantial accuracy and potential in using MASTs, but advises cautious clinical interpretation for some allergens.

Research Methodology

  • The study involved comparing serological (sIgE) tests and intradermal tests (IDTs) in fourteen atopic Malopolski horses to investigate alternative methods for diagnosing allergies.
  • A quantitative analysis was carried out to evaluate the correlation and agreement between the two types of test results.
  • Receiver operating characteristic (ROC) analysis was performed to measure the diagnostic performance of these tests.

Key Findings

  • The ROC analysis indicated that the sIgE test had the highest diagnostic performance with a 96.9% area under the ROC curve (AUC), followed by other unspecified allergens with AUCs ranging from 80.3% to 84.4%.
  • There was a significant positive correlation between IDT and MAST results for two particular allergens with correlation coefficients of 0.870 and 0.657.
  • In the case of semiquantitative assessment, there was a notable moderate agreement for two out of five allergens, while for dichotomous assessment, there was a significant fair to substantial agreement for three out of five evaluated allergens.
  • The sensitivity of the tests ranged from 44% to 89%, depending on the allergen. However, MAST proved to have higher specificity for all allergens with a range of 60% to 100%.
  • The overall accuracy of the test results averaged from 73% to 77.4% based on the Youden index.

Conclusion

  • The study suggests that the serological MAST testing methodology shows good allergen detection performance, specifically for 60% allergen sIgE in a dichotomic assessment.
  • However, it also suggests that while MAST has substantial diagnostic capabilities, certain allergens require careful clinical interpretation.

Cite This Article

APA
Wilkołek P, Szczepanik M, Sitkowski W, Rodzik B, Pluta M, Taszkun I, Gołyński M. (2019). Evaluation of multiple allergen simultaneous (sIgE) testing compared to intradermal testing in the etiological diagnosis of atopic dermatitis in horses. J Vet Sci, 20(6), e60. https://doi.org/10.4142/jvs.2019.20.e60

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 20
Issue: 6
Pages: e60
PII: e60

Researcher Affiliations

Wilkołek, Piotr
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland. pwilku@poczta.onet.pl.
Szczepanik, Marcin
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Sitkowski, Wiesław
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Rodzik, Beata
  • Institute of Mathematics, Marie Curie-Skłodowska University, 20-031 Lublin, Poland.
Pluta, Michał
  • Equine Breeding and Management Unit, Department of Biology and Animal Breeding, University of Life Sciences in Lublin, 20-950 Lublin, Poland.
Taszkun, Iwona
  • Sub-Department of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Gołyński, Marcin
  • Nicolaus Copernicus University in Torun, Faculty of Biological and Veterinary Sciences, 87-100 Torun, Poland.

MeSH Terms

  • Allergens / analysis
  • Animals
  • Dermatitis, Atopic / diagnosis
  • Dermatitis, Atopic / veterinary
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Horse Diseases / diagnosis
  • Horses
  • Intradermal Tests / methods
  • Intradermal Tests / veterinary
  • Male
  • Sensitivity and Specificity

Grant Funding

  • DS-3/2009-2017 / University of Life Sciences in Lublin

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 27 references
  1. Stepnik CT, Outerbridge CA, White SD, Kass PH. Equine atopic skin disease and response to allergen-specific immunotherapy: a retrospective study at the University of California-Davis (1991-2008).. Vet Dermatol 2012 Feb;23(1):29-35, e7.
    pubmed: 21777308doi: 10.1111/j.1365-3164.2011.01001.xgoogle scholar: lookup
  2. Loeffler A, Herrick D, Allen S, Littlewood JD. Long-term management of horses with atopic dermatitis in southeastern England: a retrospective questionnaire study of owners' perceptions.. Vet Dermatol 2018 Dec;29(6):526-e176.
    pubmed: 30175879doi: 10.1111/vde.12685google scholar: lookup
  3. Wilkołek P, Szczepanik M, Sitkowski W, Adamek Ł, Pluta M, Taszkun I, Gołyński M. A Comparison of Intradermal Skin Testing and Serum Insect Allergen-specific IgE Determination in Horses With Insect Bite Hypersensitivity From 2008 to 2016.. J Equine Vet Sci 2019 Apr;75:65-68.
    pubmed: 31002095doi: 10.1016/j.jevs.2019.01.007google scholar: lookup
  4. Popiel J, Cekiera A. Comparison of IgE test results with intradermal skin tests for dust mites and storage mites in atopic dogs.. Pol J Vet Sci 2015;18(2):351-6.
    pubmed: 26172185doi: 10.1515/pjvs-2015-0045google scholar: lookup
  5. Kang MH, Kim HJ, Jang HJ, Park HM. Sensitization rates of causative allergens for dogs with atopic dermatitis: detection of canine allergen-specific IgE.. J Vet Sci 2014 Dec;15(4):545-50.
    pmc: PMC4269597pubmed: 24962408doi: 10.4142/jvs.2014.15.4.545google scholar: lookup
  6. Wagner B. Immunoglobulin E and allergy.. Equine Vet J 2016 Jan;48(1):13-4.
    pubmed: 26663407doi: 10.1111/evj.12513google scholar: lookup
  7. Marti E, Gerber V, Wilson AD, Lavoie JP, Horohov D, Crameri R, Lunn DP, Antczak D, Björnsdóttir S, Björnsdóttir TS, Cunningham F, Dérer M, Frey R, Hamza E, Horin P, Heimann M, Kolm-Stark G, Olafsdóttir G, Ramery E, Russell C, Schaffartzik A, Svansson V, Torsteinsdóttir S, Wagner B. Report of the 3rd Havemeyer workshop on allergic diseases of the Horse, Hólar, Iceland, June 2007.. Vet Immunol Immunopathol 2008 Dec 15;126(3-4):351-61.
    pubmed: 18775570doi: 10.1016/j.vetimm.2008.07.008google scholar: lookup
  8. Wilkołek P, Sitkowski W, Szczepanik M, Adamek Ł, Pluta M, Taszkun I, Gołyński M, Malinowska A. Comparison of serum concentrations of environmental allergen-specific IgE in atopic and healthy (nonatopic) horses.. Pol J Vet Sci 2017 Dec;20(4):789-794.
    pubmed: 29611659doi: 10.1515/pjvs-2017-0099google scholar: lookup
  9. Han M, Shin S, Park H, Park KU, Park MH, Song EY. Comparison of three multiple allergen simultaneous tests: RIDA allergy screen, MAST optigen, and polycheck allergy.. Biomed Res Int 2013;2013:340513.
    pmc: PMC3591101pubmed: 23484110doi: 10.1155/2013/340513google scholar: lookup
  10. Jang WR, Nahm CH, Kim JH, Lim DH, Jang TY, Moon YS, Kim JJ. [Allergen specific IgE measurement with Polycheck Allergy: comparison of three multiple allergen simultaneous tests].. Korean J Lab Med 2009 Oct;29(5):465-72.
    pubmed: 19893357doi: 10.3343/kjlm.2009.29.5.465google scholar: lookup
  11. Rim JH, Park BG, Kim JH, Kim HS. Comparison and clinical utility evaluation of four multiple allergen simultaneous tests including two newly introduced fully automated analyzers.. Pract Lab Med 2016 Apr 1;4:50-61.
    pmc: PMC5574498pubmed: 28856193doi: 10.1016/j.plabm.2016.01.002google scholar: lookup
  12. Mueller RS, Janda J, Jensen-Jarolim E, Rhyner C, Marti E. Allergens in veterinary medicine.. Allergy 2016 Jan;71(1):27-35.
    pmc: PMC4716287pubmed: 26280544doi: 10.1111/all.12726google scholar: lookup
  13. Wallace JC, Vogelnest LJ. Evaluation of the presence of house dust mites in horse rugs.. Vet Dermatol 2010 Dec;21(6):602-7.
    pubmed: 20529010doi: 10.1111/j.1365-3164.2010.00905.xgoogle scholar: lookup
  14. Lebis C, Bourdeau P, Marzin-Keller F. Intradermal skin tests in equine dermatology: a study of 83 horses.. Equine Vet J 2002 Nov;34(7):666-71.
    pubmed: 12455836doi: 10.2746/042516402776250469google scholar: lookup
  15. Lee JH, Park KH, Kim HS, Kim KW, Sohn MH, Kim CH, Lee JS, Hong CS, Park JW. Specific IgE measurement using AdvanSure® system: comparison of detection performance with ImmunoCAP® system in Korean allergy patients.. Clin Chim Acta 2012 May 18;413(9-10):914-9.
    pubmed: 22394454doi: 10.1016/j.cca.2012.02.018google scholar: lookup
  16. Peeters LM, Janssens S, Goddeeris BM, De Keyser K, Wilson AD, Kaufmann C, Schaffartzik A, Marti E, Buys N. Evaluation of an IgE ELISA with Culicoides spp. extracts and recombinant salivary antigens for diagnosis of insect bite hypersensitivity in Warmblood horses.. Vet J 2013 Oct;198(1):141-7.
    pubmed: 23891138doi: 10.1016/j.tvjl.2013.06.010google scholar: lookup
  17. Verdon M, Lanz S, Rhyner C, Gerber V, Marti E. Allergen-specific immunoglobulin E in sera of horses affected with insect bite hypersensitivity, severe equine asthma or both conditions.. J Vet Intern Med 2019 Jan;33(1):266-274.
    pmc: PMC6335542pubmed: 30520523doi: 10.1111/jvim.15355google scholar: lookup
  18. Roberts HA, Hurcombe SD, Hillier A, Lorch G. Equine intradermal test threshold concentrations for house dust mite and storage mite allergens and identification of stable acari fauna.. Vet Dermatol 2014 Apr;25(2):124-34, e35-6.
    pubmed: 24800265doi: 10.1111/vde.12113google scholar: lookup
  19. Radwanski NE, Morris DO, Boston RC, Cerundolo R, Lee KW. Longitudinal evaluation of immunological responses to allergen-specific immunotherapy in horses with IgE associated dermatological disease, a pilot study.. Vet Dermatol 2019 Jun;30(3):255-e78.
    pubmed: 30828914doi: 10.1111/vde.12732google scholar: lookup
  20. Tahon L, Baselgia S, Gerber V, Doherr MG, Straub R, Robinson NE, Marti E. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009 Jan 15;127(1-2):85-93.
    pubmed: 19027178doi: 10.1016/j.vetimm.2008.09.021google scholar: lookup
  21. Tilley P, Sales JP, Ferreira MB. Comparison of skin prick tests with in vitro allergy tests in the characterization of horses with recurrent airway obstruction.. J Equine Vet Sci 2012;32:719–727.
  22. Lorch G, Hillier A, Kwochka KW, Saville WJ, Kohn CW, LeRoy BE. Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with and without atopy.. J Am Vet Med Assoc 2001 Apr 15;218(8):1314-22.
    pubmed: 11330620doi: 10.2460/javma.2001.218.1314google scholar: lookup
  23. Morgan EE, Miller WH Jr, Wagner B. A comparison of intradermal testing and detection of allergen-specific immunoglobulin E in serum by enzyme-linked immunosorbent assay in horses affected with skin hypersensitivity.. Vet Immunol Immunopathol 2007 Dec 15;120(3-4):160-7.
    pubmed: 17888519doi: 10.1016/j.vetimm.2007.08.007google scholar: lookup
  24. Ferroglio E, Pregel P, Accossato A, Taricco I, Bollo E, Rossi L, Trisciuoglio A. Equine Culicoides hypersensitivity: evaluation of a skin test and of humoral response.. J Vet Med A Physiol Pathol Clin Med 2006 Feb;53(1):30-3.
    pubmed: 16411906doi: 10.1111/j.1439-0442.2006.00783.xgoogle scholar: lookup
  25. Agarwal R, Aggarwal AN, Garg M, Saikia B, Chakrabarti A. Cut-off values of serum IgE (total and A. fumigatus -specific) and eosinophil count in differentiating allergic bronchopulmonary aspergillosis from asthma.. Mycoses 2014 Nov;57(11):659-63.
    pubmed: 24963741doi: 10.1111/myc.12214google scholar: lookup
  26. Wongpiyabovorn J, Suratannon N, Boonmee S, Chatchatee P. Comparison of specific IgE detection by immunoblotting and fluorescence enzyme assay with in vivo skin prick test.. Asian Pac J Allergy Immunol 2018 Sep;36(3):159-165.
    pubmed: 28938843doi: 10.12932/ap-270217-0035google scholar: lookup
  27. Wilkołek PM, Pomorski ZJ, Szczepanik MP, Adamek L, Pluta M, Taszkun I, Gołyński M, Rozwód A, Sitkowski W. Assessment of serum levels of allergen-specific immunoglobulin E in different seasons and breeds in healthy horses.. Pol J Vet Sci 2014;17(2):331-7.
    pubmed: 24988860doi: 10.2478/pjvs-2014-0045google scholar: lookup

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