Evaluation of neutrophil apoptosis in horses with acute abdominal disease.
Abstract: To quantify peripheral blood neutrophil apoptosis in equine patients with acute abdominal disease (ie, colic) caused by strangulating or nonstrangulating intestinal lesions and compare these values with values for horses undergoing elective arthroscopic surgery. Methods: 20 client-owned adult horses. Methods: Peripheral blood was collected from horses immediately prior to and 24 hours after surgery for treatment of colic (n = 10) or elective arthroscopic surgery (10), and neutrophils were counted. Following isolation by means of a bilayer colloidal silica particle gradient and culture for 24 hours, the proportion of neutrophils in apoptosis was detected by flow cytometric evaluation of cells stained with annexin V and 7-aminoactinomycin D. Values were compared between the colic and arthroscopy groups; among horses with colic, values were further compared between horses with and without strangulating intestinal lesions. Results: Percentage recovery of neutrophils was significantly smaller in preoperative samples (median, 32.5%) and in all samples combined (35.5%) for the colic group, compared with the arthroscopy group (median, 66.5% and 58.0%, respectively). No significant differences in the percentages of apoptotic neutrophils were detected between these groups. Among horses with colic, those with strangulating intestinal lesions had a significantly lower proportion of circulating apoptotic neutrophils in postoperative samples (median, 18.0%) than did those with nonstrangulating lesions (66.3%). Conclusions: The smaller proportion of apoptotic neutrophils in horses with intestinal strangulation suggested that the inflammatory response could be greater or prolonged, compared with that of horses with nonstrangulating intestinal lesions. Further investigations are needed to better understand the relationship between neutrophil apoptosis and inflammation during intestinal injury.
Publication Date: 2013-06-28 PubMed ID: 23802671DOI: 10.2460/ajvr.74.7.999Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the degree of white blood cell death (neutrophil apoptosis) in horses suffering from acute abdominal disease, specifically colic, to understand the correlation between inflammation and tissue damage.
Study Description and Methodology
In this study:
- The research was conducted on 20 adult horses, 10 suffering from colic and another 10 undergoing elective arthroscopic surgery.
- The objective was to quantify the amount of neutrophil apoptosis, which is a process leading to the death of neutrophils, a kind of white blood cell crucial in the body’s defence system.
- Blood samples were collected before and after surgery, and neutrophils were counted.
- Neutrophils were isolated using a bilayer colloidal silica particle gradient and cultured for 24 hours.
- The proportion of neutrophils undergoing apoptosis was detected using flow cytometry techniques, staining cells with annexin V and 7-aminoactinomycin D.
- The numerical values were compared between the group of horses suffering from colic and those undergoing arthroscopic surgery.
Results of the Study
The study findings indicated that:
- Horses suffering from colic had a significantly lesser neutrophil recovery rate in the preoperative samples (32.5%) and in all combined samples (35.5%) compared to the arthroscopy group (66.5% and 58.0% respectively).
- No significant difference in the percentage of apoptotic neutrophils was found between the colic group and the arthroscopic group.
- In the colic group, horses with strangulating intestinal lesions showed a significantly lower proportion of circulating apoptotic neutrophils in postoperative samples (18.0%) compared to those with non-strangulating lesions (66.3%)
Conclusions of the Study
Based on the results, the following conclusions were drawn:
- The lower proportion of apoptotic neutrophils in horses with intestinal strangulation suggested that the inflammatory response could be more severe or last longer compared to horses with non-strangulating intestinal lesions.
- Further studies are needed to fully comprehend the relationship between neutrophil apoptosis and inflammation during intestinal injury.
Cite This Article
APA
Krista KM, White NA, Barrett JG, Furr MO, Buechner-Maxwell VA.
(2013).
Evaluation of neutrophil apoptosis in horses with acute abdominal disease.
Am J Vet Res, 74(7), 999-1004.
https://doi.org/10.2460/ajvr.74.7.999 Publication
Researcher Affiliations
- Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
MeSH Terms
- Animals
- Apoptosis / physiology
- Cell Culture Techniques
- Colic / metabolism
- Colic / pathology
- Colic / veterinary
- Female
- Horse Diseases / metabolism
- Horses
- Male
- Neutrophils / metabolism
Citations
This article has been cited 2 times.- Bayless RL, Sheats MK, Jones SL. Withaferin A Inhibits Neutrophil Adhesion, Migration, and Respiratory Burst and Promotes Timely Neutrophil Apoptosis. Front Vet Sci 2022;9:900453.
- Sheats MK. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils. Front Vet Sci 2019;6:69.
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