Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea.
Abstract: In this study, we aimed to investigate the recent changes such as allele frequencies and total probability of exclusion (PE) in Thoroughbred horses in Korea using short tandem repeat (STR) parentage panels between 2006 and 2016. Methods: The genotype was provided for 5,988 horse samples with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). Results: In our study, the observed number of alleles per locus ranged from 3 (HMS1) to 9 (ASB17) in 2006 and 4 (HMS1) to 9 (ASB2) in 2016, with a mean value of 6.28 and 6.40, respectively. Of the 15 markers, HMS2, HTG4, and CA425 loci had relatively low polymorphism information content (<0.5000) in the Thoroughbred population. Mean levels of genetic variation in 2006 and 2016 were observed heterozygosity (HO) = 0.708, and expected heterozygosity (HE) = 0.685, as well as and HO = 0.699 and HE = 0.682, respectively. The PE was calculated for each group based on the allele frequencies of 14 or 15 STRs. The 2006 survey analyzed that PE was 0.9998, but it increased to 0.9999 in 2016 after the HMS2 marker was added in 2011. The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred population. Conclusions: The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred horses. However, continuous monitoring genetic variability is necessary.
Publication Date: 2021-08-25 PubMed ID: 34530514PubMed Central: PMC8902210DOI: 10.5713/ab.21.0272Google Scholar: Lookup
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- Journal Article
Summary
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This study examines the changes in allele frequencies and genetic variability in Thoroughbred horses in Korea over the decade between 2006 and 2016, using microsatellite markers. The researchers concluded that their current method is effective for identifying parentage, but ongoing monitoring of genetic variability is required.
Methods and Data Collection
- Genotype data were collected from nearly 6,000 Korean Thoroughbred horse samples.
- The researchers used 15 specific microsatellite markers, which are sections of DNA that contain short sequences repeated many times.
- The aim was to track the frequency of these specific markers (or alleles) over the decade-long period, to monitor genetic changes in the horse population.
Results
- The frequency of these alleles varied, with anywhere from 3 to 9 observed per genetic locus in 2006, and 4 to 9 per locus in 2016.
- Three of the markers (HMS2, HTG4, and CA425) showed relatively low polymorphism information content, meaning there was less variation in these genes in the Thoroughbred population.
- Statistical measures of observed heterozygosity (HO; variety within individual’s genes) and expected heterozygosity (HE; expected genetic diversity) were calculated and found to be similar in 2006 and 2016.
- The total probability of exclusion (PE), a measure used to determine if a certain parent can be excluded as the biological parent of an offspring based on genetic markers, was also calculated. It was found to be high in both 2006 and 2016, increasing slightly after the addition of the HMS2 marker in 2011.
Conclusions
- The researchers concluded that the current panel of microsatellite markers is a powerful tool for verifying parentage in the Thoroughbred horse population, which is important for maintaining the integrity of the breed.
- However, they emphasized the importance of ongoing monitoring of genetic variability in the population to ensure long-term breed health and diversity.
Cite This Article
APA
Park CS, Lee SY, Cho GJ.
(2021).
Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea.
Anim Biosci, 35(4), 527-532.
https://doi.org/10.5713/ab.21.0272 Publication
Researcher Affiliations
- College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, Korea.
- Racing Laboratory, Korea Racing Association, Gwacheon 13822, Korea.
- College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, Korea.
Grant Funding
- 2020R1I1A3067905 / National Research Foundation of Korea
- Ministry of Education, Science and Technology
Conflict of Interest Statement
. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.
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Citations
This article has been cited 4 times.- Kim D, Lee S, Oyungerel B, Cho G. Evaluation of the Effectiveness of Single-Nucleotide Polymorphisms Versus Microsatellites for Parentage Verification in Horse Breeds. Vet Sci 2025 Sep 15;12(9).
- Kulibaba RO, Srikulnath K, Singchat W, Liashenko YV, Griffin DK, Romanov MN. The Application of Microsatellite Markers as Molecular Tools for Studying Genomic Variability in Vertebrate Populations. Curr Issues Mol Biol 2025 Jun 11;47(6).
- Feng H, Cao F, Jin T, Wang L. Forest fragmentation causes an isolated population of the golden takin (Budorcas taxicolor bedfordi Thomas, 1911) (Artiodactyla: Bovidae) in the Qinling Mountains (China). BMC Zool 2024 Jan 30;9(1):2.
- Lee SY, Kim SM, Oyungerel B, Cho GJ. Single nucleotide polymorphisms for parentage testing of horse breeds in Korea. Anim Biosci 2024 Apr;37(4):600-608.
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