Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses: Part I–precision, linearity, and accuracy of complete blood cell count.

The research article focuses on a comparison study between the Sysmex XT-2000iV™, a laser-based hematology analyzer, and its predecessor the ADVIA® 2120, with the objective of determining the new device’s precision, linearity, and accuracy in regard to complete blood cell count on dogs, cats, and horses.

Methodology

• The aim of the study was to assess three principal attributes of the Sysmex Analyzer: precision, linearity, and accuracy.
• The ADVIA® 2120 analyzer was used as the reference point (standard) for the accuracy study.
• The study included fresh blood samples from healthy and diseased cats (n = 216), dogs (n = 314), and horses (n = 174) to evaluate the accuracy of the Sysmex Analyzer.

Findings

• The study found a low intra-assay coefficient of variation (CV) of about 1% for the complete blood cell count (CBC) excluding platelet count (PLT).
• An intra-assay CV between 2% and 5.5% was observed for the differential count, except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%).
• The linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT.

Correlation and Agreement

• The agreement between the Sysmex and ADVIA analyzers was excellent for WBC and RBC, with Spearman rank correlation coefficients (rs) ranging from more than 0.99 to 0.98 for all the evaluated species.
• In cats and dogs, the hematocrit value showed excellent correlation.
• Platelet count in felines and equines displayed good correlation (rs = 0.89 and 0.92, respectively), while in dogs it was excellent (rs = 0.93).

Overall Outcome

• Biases were almost negligible except for Mean Corpuscular Hemoglobin Concentration (4.11 to -7.25 mmol/l) and canine Platelet count (57 × 10(9)/l).
• Overall, the performance of the Sysmex Analyzer was excellent, as it compared favorably with the ADVIA analyzer.

The study concludes that the Sysmex Analyzer is an effective and accurate tool for complete blood cell count in dogs, cats, and horses. Despite some minor discrepancies, it has the potential to replace the older model, ADVIA analyzer.