Evaluation of the automated hematology analyzer Sysmex XT-2000iV ™ compared to the ADVIA ® 2120 for its use in dogs, cats, and horses. Part II: Accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging.
Abstract: The automated laser-based hematology analyzer Sysmex XT-2000iV™ provides a 5-part differential count and specific cytograms that are of great interest for large veterinary laboratories. The aim of the study was to validate the Sysmex XT-2000iV compared to the laser-based hematology analyzer ADVIA® 2120 and manual differential in dogs, cats, and horses as well as the impact of anticoagulant (heparin, ethylenediamine tetra-acetic acid [EDTA], and citrate) and storage at 22°C and 4°C. Consecutive fresh K(3)-EDTA blood samples from 216 cats, 314 dogs, and 174 horses were included. The impact of anticoagulant and sample storage was assessed in specimens obtained from an additional 9 cats, 10 dogs, and 10 horses. Agreement between both analyzers was excellent to good except for monocytes and canine reticulocytes. Spearman rank correlation coefficients (r (s)) between Sysmex XT-2000iV and manual differential were good to fair and ranged from 0.91 (cat lymphocytes) to 0.44 (cat monocytes). Hematocrit value (Hct), mean corpuscular hemoglobin (MCH), MCH concentration (MCHC; all: P < 0.001), and mean corpuscular volume (MCV; P < 0.01) were higher in canine citrated blood compared to heparin and EDTA. In cats, lymphocytes and monocytes were lower in heparinized blood compared to EDTA (P < 0.05), whereas in horses no significant effect was seen. Regarding storage time and temperature, white and red blood cell counts, hemoglobin, and MCH were stable. Hct, MCV, and MCHC were influenced by erythrocyte swelling. Differential count remained stable for 24 hr (22°C) and nearly 72 hr (4°C) except for monocytes. The overall performance of the Sysmex XT-2000iV was excellent and compared favorably with that of the ADVIA 2120. A special strength was the excellent detection of feline eosinophils.
Publication Date: 2012-03-01 PubMed ID: 22362937DOI: 10.1177/1040638711436243Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Analytical Methods
- Animal Health
- Blood Analysis
- Cells
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Imaging
- Diagnostic Technique
- Disease Diagnosis
- Equine Health
- Erythrocytes
- Hematology
- Horses
- In Vitro Research
- Laboratory Methods
- Leukocytes
- Veterinary Medicine
- Veterinary Procedure
- Veterinary Research
Summary
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This research focused on the evaluation of the Sysmex XT-2000iV, an automated laser-based hematology analyzer, for its application in veterinary laboratories, specifically for dogs, cats, and horses. The analyzer provides a 5-part differential count and specific cytograms. It was compared to the ADVIA 2120 and manual differential, considering the influence of specific anticoagulants and sample storage conditions. On the whole, the analyzer’s performance compared favorably to the ADVIA 2120, with noteworthy precision in detecting feline eosinophils.
Objective of Study and Analyzers Compared
- The study sought to validate the performance and accuracy of the Sysmex XT-2000iV automated laser-based hematology analyzer in comparison with the ADVIA 2120 hematology analyzer and manual differential.
- The Sysmex XT-2000iV is notable for its detailed 5-part differential count and specific cytograms.
- These systems were compared based on their use in dogs, cats, and horses.
Sample Selection and Storage Conditions
- Blood samples were collected from 216 cats, 314 dogs, and 174 horses for this study.
- The study also looked at the effect of anticoagulant use—the effect of heparin, ethylenediamine tetra-acetic acid (EDTA), and citrate was assessed—and temperature storage conditions (22°C and 4°C) on the results.
- A sample composed of blood samples from an additional 9 cats, 10 dogs, and 10 horses were used to determine this impact.
Validation Results and Impact of Anticoagulated and Stored Samples
- Results showed excellent to good agreement between the two analyzers, except for monocytes and canine reticulocytes.
- The impact of anticoagulant samples in dogs showed higher hematocrit value (Hct), mean corpuscular hemoglobin (MCH), and MCH concentration (MCHC) in samples with canine citrated blood in contrast with samples with heparin and EDTA.
- The study also found that in cats, a lower lymphocyte and monocyte count was seen in samples of heparinized blood as opposed to those with EDTA. In horses, no significant impact was observed due to anticoagulant use.
- The study findings indicate that certain key factors, including white and red blood cell counts, hemoglobin, and MCH, remain stable regardless of storage time and temperature. However, factors such as Hct, MCV, and MCHC were influenced by erythrocyte swelling.
- The differential count remained stable for 24 hours at 22°C and nearly 72 hours at 4°C, with the exception of monocytes.
Overall Performance of Sysmex XT-2000iV
- This study found the performance of the Sysmex XT-2000iV to be excellent and comparable to the ADVIA 2120 for use in large veterinary laboratories.
- One special strength demonstrated by the Sysmex XT-2000iV is the precise detection of feline eosinophils.
Cite This Article
APA
Bauer N, Nakagawa J, Dunker C, Failing K, Moritz A.
(2012).
Evaluation of the automated hematology analyzer Sysmex XT-2000iV ™ compared to the ADVIA ® 2120 for its use in dogs, cats, and horses. Part II: Accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging.
J Vet Diagn Invest, 24(1), 74-89.
https://doi.org/10.1177/1040638711436243 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University Giessen, Frankfurterstr. 129, 35392 Giessen, Germany. Natali.Bauer@vetmed.uni-giessen.de
MeSH Terms
- Animals
- Anticoagulants
- Autoanalysis / instrumentation
- Autoanalysis / veterinary
- Blood Preservation / methods
- Blood Preservation / veterinary
- Cat Diseases / blood
- Cat Diseases / diagnosis
- Cats
- Dog Diseases / blood
- Dog Diseases / diagnosis
- Dogs
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Leukocyte Count / instrumentation
- Leukocyte Count / veterinary
- Reproducibility of Results
- Reticulocyte Count / instrumentation
- Reticulocyte Count / veterinary
- Temperature
- Time Factors
Citations
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