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Home / Equine Research Bank / J Reprod Dev / Evaluation of the chemiluminescent enzyme immunoassay system...
The Journal of reproduction and development2017; 64(1); 41-47; doi: 10.1262/jrd.2017-099

Evaluation of the chemiluminescent enzyme immunoassay system for the measurement of testosterone in the serum and whole blood of stallions.

Abstract: Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions. The serum T concentrations measured by the two methods were highly correlated (r = 0.9865, n = 56). Second, to validate the use of whole blood as assay samples, T concentrations in whole blood and in the serum were measured by the CLEIA system. T concentrations in both samples were highly correlated (r = 0.9665, n = 64). Finally, to evaluate the practical value of the CLEIA system in clinical settings, T concentrations were measured in three stallions with reproductive abnormalities after the administration of human chorionic gonadotropin (hCG). Two stallions with small or absent testes in the scrotum showed an increase in T production in response to hCG administration and one stallion with seminoma did not. In conclusion, the CLEIA system was found to be a rapid and reliable tool for measuring T concentrations in stallions and may improve reproductive management in clinical settings and in breeding studs.
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Publication Date: 2017-11-11 PubMed ID: 29129877PubMed Central: PMC5830357DOI: 10.1262/jrd.2017-099Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study presents the validation and utility of a rapid chemiluminescent enzyme immunoassay system (CLEIA system) for measuring testosterone concentrations in stallion blood samples.

Objective of the Study

  • The study aimed to evaluate the effectiveness and accuracy of the Chemiluminescent Enzyme Immunoassay System (CLEIA System) that is primarily designed for humans, in measuring testosterone concentration in stallions (male horses).

Methodology

  • The research first involved measuring serum testosterone concentrations using the CLEIA system, and comparing these results with those measured by a different method (fluoroimmunoassay) that had already been validated for use in stallions.
  • To validate the use of whole blood as assay samples, testosterone concentrations in whole blood and in the serum were measured by the CLEIA system.
  • The practical application of the CLEIA system was evaluated in a clinical setting by measuring testosterone concentrations in three stallions diagnosed with reproductive abnormalities, post-administration of human chorionic gonadotropin (hCG).

Findings

  • The results using the CLEIA system and the fluoroimmunoassay were found to be highly correlated, supporting the reliability of the CLEIA system for horse samples.
  • Correlation was also observed between the testosterone concentrations in whole blood and in the serum, methods measured by the CLEIA system, validating the use of whole blood as an assay sample.
  • The practicality of the CLEIA system in a clinical setting was demonstrated when two out of three stallions showed an increase in testosterone production, in response to the administration of hCG, while one did not respond possibly due to the presence of a seminoma (a form of testicular tumor).

Conclusion

  • The study concluded that the CLEIA system provides reliable and rapid results for measuring testosterone concentrations in stallions.
  • The CLEIA system could help in improving reproductive management in clinical settings and in breeding studs.

Cite This Article

APA
Toishi Y, Tsunoda N, Nagata SI, Kirisawa R, Nagaoka K, Watanabe G, Yanagawa Y, Katagiri S, Taya K. (2017). Evaluation of the chemiluminescent enzyme immunoassay system for the measurement of testosterone in the serum and whole blood of stallions. J Reprod Dev, 64(1), 41-47. https://doi.org/10.1262/jrd.2017-099

Publication

The Journal of reproduction and development
ISSN: 1348-4400
NlmUniqueID: 9438792
Country: Japan
Language: English
Volume: 64
Issue: 1
Pages: 41-47

Researcher Affiliations

Toishi, Yuko
  • Shadai Stallion Station, Hokkaido 059-1432, Japan.
  • Laboratory of Veterinary Virology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido 069-8501, Japan.
Tsunoda, Nobuo
  • Shadai Stallion Station, Hokkaido 059-1432, Japan.
Nagata, Shun-Ichi
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
Kirisawa, Rikio
  • Laboratory of Veterinary Virology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido 069-8501, Japan.
Nagaoka, Kentaro
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
  • United Graduated School of Veterinarian Science, Gifu University, Gifu 501-1193, Japan.
Watanabe, Gen
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
  • United Graduated School of Veterinarian Science, Gifu University, Gifu 501-1193, Japan.
Yanagawa, Yojiro
  • Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0808, Japan.
Katagiri, Seiji
  • Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0808, Japan.
Taya, Kazuyoshi
  • Shadai Stallion Station, Hokkaido 059-1432, Japan.
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.

MeSH Terms

  • Animals
  • Horses
  • Immunoenzyme Techniques / methods
  • Luminescent Measurements / methods
  • Male
  • Testosterone / blood

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Citations

This article has been cited 1 times.
  1. Murase H, Ochi A, Tozaki T, Kakoi H, Munkhtuul T, Kurimoto S, Sato F, Hada T. A case of equine cryptorchidism with undetectable serum anti-Müllerian hormone.. J Vet Med Sci 2020 Feb 18;82(2):209-211.
    doi: 10.1292/jvms.18-0057pubmed: 31875577google scholar: lookup
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