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Evaluation of the PATHFAST Chemiluminescent Enzyme Immunoassay for Measuring Progesterone in Whole Blood and Serum of Mares.
Abstract: Evaluation of a new chemiluminescent enzyme immunoassay, the PATHFAST assay system (PATHFAST), for measurement of circulating progesterone in mares was performed. Five mares at the mid-luteal stage were administrated a single i.m. injection of prostaglandin F2α analog (PGF2α; cloprostenol 250 μg/ml), and then blood samples were collected from the jugular vein at 0, 15, 30 and 45 min, at one-hour intervals until 24 and at 48 hr via a catheter in the jugular vein. To monitor the physiological changes in circulating progesterone in mares after induced luteolysis, concentrations of progesterone in whole blood and serum samples were measured by PATHFAST. In addition, concentrations of progesterone in serum samples measured by PATHFAST were compared with those measured by radioimmunoassay (RIA) and enzyme immunoassay (EIA). Using PATHFAST, the serum concentrations of progesterone in mares correlated highly with those of whole blood samples (r=0.9672, n=88). The serum concentrations of progesterone as measured by PATHFAST correlated well with RIA (r=0.9654, n=88) and EIA (r=0.9323, n=112). An abrupt decline in circulating progesterone in whole blood samples was observed within 2 hr (50%), followed by a gradual decline until 48 hr later. The results for progesterone in whole blood samples correlated highly with those in serum samples, and the declining pattern paralleled that of the serum samples. These results demonstrated that PATHFAST is useful in the equine clinic as an accurate diagnostic tool for rapid assay of progesterone within 26 min, using unextracted whole blood.
Publication Date: 2013-09-30 PubMed ID: 24834001PubMed Central: PMC4013986DOI: 10.1294/jes.24.47Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study explored the use of a new immunoassay, PATHFAST, for testing progesterone levels in horses. The research found that the assay could accurately detect changes in progesterone levels following a hormonal treatment, demonstrating that the assay could be a practical tool in equine clinics.
Research aims and methodology
- The main aim of this study was to evaluate the potential of the PATHFAST chemiluminescent enzyme immunoassay as a diagnostic tool to measure progesterone in horses (mares).
- Five mid-luteal stage mares were administered a prostaglandin F2α analog injection and then had blood samples taken at various time intervals over 48 hours.
- Progesterone concentrations in both whole blood and serum samples were measured using PATHFAST.
- These concentrations were then compared with those measured through two other established testing methods: radioimmunoassay (RIA) and enzyme immunoassay (EIA).
Results and findings
- The researchers found that the PATHFAST assay was able to accurately detect levels of progesterone in both whole blood and serum samples, exhibiting a high correlation between the two sample types.
- Furthermore, PATHFAST results showed a strong correspondence with results from RIA and EIA, which are proven testing methodologies. This emphasizes the reliability of the new assay.
- The study recorded a sharp decline in progesterone levels in whole blood samples within two hours of the hormonal treatment, followed by a more gradual decline up to 48 hours later.
Implications and conclusions
- The findings suggest that the PATHFAST assay is an effective tool for quickly assessing progesterone levels in horses, needing only 26 minutes for an assay using unextracted whole blood.
- This augments the significance of PATHFAST as an excellent diagnostic tool in equine clinics for measuring progesterone, offering advantages in terms of speed, accuracy, and convenience.
Cite This Article
APA
Toishi Y, Tsunoda N, Tagami M, Hashimoto H, Kato F, Suzuki T, Nagaoka K, Watanabe G, Tokuyama S, Okuda K, Taya K.
(2013).
Evaluation of the PATHFAST Chemiluminescent Enzyme Immunoassay for Measuring Progesterone in Whole Blood and Serum of Mares.
J Equine Sci, 24(3), 47-51.
https://doi.org/10.1294/jes.24.47 Publication
Researcher Affiliations
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
- Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan ; Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
- Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan ; Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
- Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
- Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.
- Shadai Corporation, Hokkaido 059-1432, Japan.
References
This article includes 5 references
- Chuko M, Otsuki J, Nagai Y. Raid whole blood assay on PATHFAST for quantification of gonadal hormones and the clinical evaluation in assisted reproductive technology. Jpn. J. Med. Pham. Sci. 2010;63:111–119.
- Maeda K, Otuki J, Terai K, Nagai Y. Rapid whole blood assay on PATHFAST for quantification of testosterone and the clinical evaluation in assisted reproductive technology. Jpn. J. Med. Pham. Sci. 2011;66:693–700.
- Okuda K, Uenoyama Y, Fujita Y, Iga K, Sakamoto K, Kimura T. Functional oxytocin receptors in bovine granulosa cells.. Biol Reprod 1997 Mar;56(3):625-31.
- Sugie Y, Igami K, Shoji K, Arai N, Tazaki Y, Kouta H, Okamura Y, Tashiro S, Yokoi H. Performance evaluation of the new rapid fertility assays in whole blood and plasma on PATHFAST.. Clin Lab 2011;57(1-2):99-106.
- Taya K, Watanabe G, Sasamoto S. Radioimmunoassay for progesterone, testosterone and estradiol-17β using 125I-iodohistamine radioligands. Jpn. J. Anim. Reprod. 1985;31:186–197.
Citations
This article has been cited 2 times.- Satoh M, Higuchi T, Inoue S, Gotoh T, Murase H, Nambo Y. Factors affecting the prognosis for uterine torsion: the effect of treatment based on measurements of serum progesterone and estradiol concentrations after surgery. J Equine Sci 2017;28(4):163-167.
- Toishi Y, Tsunoda N, Kume K, Nagaoka K, Watanabe G, Taya K. PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum. J Reprod Dev 2016 Dec 20;62(6):631-634.
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