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Pathogens (Basel, Switzerland)2021; 10(4); 425; doi: 10.3390/pathogens10040425

Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea.

Abstract: Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7-100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701-818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.
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Publication Date: 2021-04-02 PubMed ID: 33918404PubMed Central: PMC8066002DOI: 10.3390/pathogens10040425Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to understand the genetic makeup of the Equine herpesvirus-1 (EHV-1), a common horse pathogen in South Korea, by comparing isolated samples with existing genetic sequences. The study found that all the isolated viruses were of a non-neuropathogenic type and were classified into two groups based on ORF34 and multi-locus sequence typing (MLST) analysis.

Objective and Methodology

  • The researchers aimed to understand the genetic variability of the equine herpesvirus-1 (EHV-1) found in South Korea. This was done by collecting samples from 338 horses, out of which 12 EHV-1 were isolated.
  • The genetic makeup of these isolates was then compared to existing sequence data using the ORF30, ORF33, ORF68, and ORF34 genes and multi-locus sequence typing (MLST).

Findings

  • The study confirmed that all isolated viruses belonged to the non-neuropathogenic type, indicated by N752 of the ORF30 gene and a highly conserved ORF33 gene (99.7-100%). This type doesn’t cause damage to the nervous system of the horse.
  • The researchers observed a 118 base pair (bp) deletion in the nucleotide sequences 701-818 of the ORF68 gene which made it impossible to classify the isolates using ORF68 analysis. Sequence deletions like this can often disrupt the functioning of the resulting protein and could be a significant factor in the pathogenicity or virulence of the virus.
  • On analysis of the ORF34 gene and multi-locus sequence typing (MLST), the EHV-1 isolates were classified into two groups: one group comprised of seven isolates, falling under group 1, clade 10, and the other group included the remaining five isolates which fell under group 7, clade 6. This kind of grouping allows scientists to understand transmission patterns and evolutionary relationships between different virus isolates.

Significance

  • The research provides valuable insights into the genetic makeup of the EHV-1 virus in South Korea, thus improving the understanding of this widespread horse pathogen.
  • Finding sequence variability and groupings assist in tracking virus evolution and transmission patterns, information that can be pivotal in controlling the incidence and spread of the disease in equine populations.
  • Identification of a non-neuropathogenic type of EHV-1 adds to the understanding of the kind of EHV-1 strains predominant in South Korean horses, providing key details that can guide treatment and prevention strategies.

Cite This Article

APA
Kang HW, Lee EY, Lee KK, Ko MK, Park JY, Kim YH, Lee K, Choi EJ, Kim J, So B, Park CK, Jeoung HY. (2021). Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea. Pathogens, 10(4), 425. https://doi.org/10.3390/pathogens10040425

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 4
PII: 425

Researcher Affiliations

Kang, Hyung-Woo
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
  • College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Korea.
Lee, Eun-Yong
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Lee, Kyoung-Ki
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Ko, Mi-Kyeong
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Park, Ji-Young
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Kim, Yeon-Hee
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Lee, Kyunghyun
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Choi, Eun-Jin
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Kim, Jongho
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
So, ByungJae
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Park, Choi-Kyu
  • College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Korea.
Jeoung, Hye-Young
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.

Grant Funding

  • B-1543069-2019-20-03 / Animal and Plant Quarantine Agency

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 39 references
  1. Malik P, Bálint A, Dán A, Pálfi V. Molecular characterisation of the ORF68 region of equine herpesvirus-1 strains isolated from aborted fetuses in Hungary between 1977 and 2008.. Acta Vet Hung 2012 Mar;60(1):175-87.
    doi: 10.1556/avet.2012.015pubmed: 22366142google scholar: lookup
  2. Telford EA, Watson MS, McBride K, Davison AJ. The DNA sequence of equine herpesvirus-1.. Virology 1992 Jul;189(1):304-16.
    doi: 10.1016/0042-6822(92)90706-Upubmed: 1318606google scholar: lookup
  3. Stasiak K, Dunowska M, Hills SF, Rola J. Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland.. Arch Virol 2017 Aug;162(8):2329-2335.
    doi: 10.1007/s00705-017-3376-3pmc: PMC5506511pubmed: 28451902google scholar: lookup
  4. Kydd JH, Slater J, Osterrieder N, Lunn DP, Antczak DF, Azab W, Balasuriya U, Barnett C, Brosnahan M, Cook C, Damiani A, Elton D, Frampton A, Gilkerson J, Goehring L, Horohov D, Maxwell L, Minke J, Morley P, Nauwynck H, Newton R, Perkins G, Pusterla N, Soboll-Hussey G, Traub-Dargatz J, Townsend H, Van de Walle GR, Wagner B. Third International Havemeyer Workshop on Equine Herpesvirus type 1.. Equine Vet J 2012 Sep;44(5):513-7.
    doi: 10.1111/j.2042-3306.2012.00604.xpubmed: 22888774google scholar: lookup
  5. Sutton GA, Viel L, Carman PS, Boag BL. Pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo.. Can J Vet Res 1998 Jan;62(1):49-55.
    pmc: PMC1189442pubmed: 9442940
  6. Walker C, Love DN, Whalley JM. Comparison of the pathogenesis of acute equine herpesvirus 1 (EHV-1) infection in the horse and the mouse model: a review.. Vet Microbiol 1999 Aug 16;68(1-2):3-13.
    doi: 10.1016/S0378-1135(99)00056-5pubmed: 10501157google scholar: lookup
  7. Szeredi L, Tenk M, Jánosi S, Pálfi V, Hotzel H, Sachse K, Pospischil A, Bozsó M, Glávits R, Molnár T. A survey of equine abortion and perinatal foal losses in Hungary during a three-year period (1998-2000).. Acta Vet Hung 2008 Sep;56(3):353-67.
    doi: 10.1556/avet.56.2008.3.9pubmed: 18828487google scholar: lookup
  8. Szeredi L, Pálfi V, Molnár T. Comparison of methods for the diagnosis of equine herpesvirus type 1 infection.. Acta Vet Hung 2003;51(2):153-63.
    doi: 10.1556/avet.51.2003.2.3pubmed: 12737042google scholar: lookup
  9. Borchers K, Slater J. A nested PCR for the detection and differentiation of EHV-1 and EHV-4.. J Virol Methods 1993 Dec 31;45(3):331-6.
    doi: 10.1016/0166-0934(93)90117-Apubmed: 8106604google scholar: lookup
  10. Nugent J, Birch-Machin I, Smith KC, Mumford JA, Swann Z, Newton JR, Bowden RJ, Allen GP, Davis-Poynter N. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks.. J Virol 2006 Apr;80(8):4047-60.
    doi: 10.1128/JVI.80.8.4047-4060.2006pmc: PMC1440451pubmed: 16571821google scholar: lookup
  11. Smith KL, Allen GP, Branscum AJ, Frank Cook R, Vickers ML, Timoney PJ, Balasuriya UB. The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions.. Vet Microbiol 2010 Feb 24;141(1-2):5-11.
    doi: 10.1016/j.vetmic.2009.07.030pubmed: 19733451google scholar: lookup
  12. Spiesschaert B, Osterrieder N, Azab W. Comparative analysis of glycoprotein B (gB) of equine herpesvirus type 1 and type 4 (EHV-1 and EHV-4) in cellular tropism and cell-to-cell transmission.. Viruses 2015 Feb 3;7(2):522-42.
    doi: 10.3390/v7020522pmc: PMC4353902pubmed: 25654240google scholar: lookup
  13. Lee SK, Lee JK, Lee I. Molecular Detection of Equus caballus Papillomavirus Type 2 in Genital Swabs From Healthy Horses in the Republic of Korea.. J Equine Vet Sci 2019 Jan;72:97-100.
    doi: 10.1016/j.jevs.2018.10.015pubmed: 30929791google scholar: lookup
  14. Goehring LS, van Winden SC, van Maanen C, Sloet van Oldruitenborgh-Oosterbaan MM. Equine herpesvirus type 1-associated myeloencephalopathy in The Netherlands: a four-year retrospective study (1999-2003).. J Vet Intern Med 2006 May-Jun;20(3):601-7.
    doi: 10.1111/j.1939-1676.2006.tb02903.xpubmed: 16734096google scholar: lookup
  15. Garvey M, Lyons R, Hector RD, Walsh C, Arkins S, Cullinane A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017.. Pathogens 2019 Jan 15;8(1).
    doi: 10.3390/pathogens8010007pmc: PMC6471309pubmed: 30650561google scholar: lookup
  16. Preziuso S, Sgorbini M, Marmorini P, Cuteri V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes.. Viruses 2019 Sep 13;11(9).
    doi: 10.3390/v11090851pmc: PMC6784080pubmed: 31540321google scholar: lookup
  17. Bryant NA, Wilkie GS, Russell CA, Compston L, Grafham D, Clissold L, McLay K, Medcalf L, Newton R, Davison AJ, Elton DM. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks.. Transbound Emerg Dis 2018 Jun;65(3):817-832.
    doi: 10.1111/tbed.12809pmc: PMC5947664pubmed: 29423949google scholar: lookup
  18. Bak U.-B., Lim C.H., Kang B.H., Lee S.Y.. A pathological survey on equine viral rhinopneumonitis occurred in Korea.. Korean J. Vet. Res. 1981;21:11–23.
  19. Moon H., Kang W.-C., Kim E.-J., Kim J.-H., Ko H.-J., Yang J.-H., Son W.-G., Lee D.-S.. Detection of equine herpesvirus type-1 in naturally aborted equine fetuses in Jeiu by polymerase chain reaction.. Korean J. Vet. Serv. 2001;24:83–88.
  20. Choi S.-K., Kim J.-H., Cho G.-J.. A diagnosis of equine herpesvirus type 1 (EHV-1) myeloencephalopathy using real-time PCR.. Korean J. Vet. Serv. 2014;37:59–65.
    doi: 10.7853/kjvs.2014.37.1.59google scholar: lookup
  21. Jung JY, Lee KH, Rhyoo MY, Byun JW, Bae YC, Choi E, Kim C, Jean YH, Lee MH, Yoon SS. Meningoencephalitis caused by Halicephalobus gingivalis in a thoroughbred gelding.. J Vet Med Sci 2014 Mar 1;76(2):281-4.
    doi: 10.1292/jvms.13-0437pmc: PMC3982813pubmed: 24107465google scholar: lookup
  22. Sutton G, Garvey M, Cullinane A, Jourdan M, Fortier C, Moreau P, Foursin M, Gryspeerdt A, Maisonnier V, Marcillaud-Pitel C, Legrand L, Paillot R, Pronost S. Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion.. Viruses 2019 Oct 4;11(10).
    doi: 10.3390/v11100916pmc: PMC6832873pubmed: 31590336google scholar: lookup
  23. Seo MG, Ouh IO, Lee SK, Lee JS, Kwon OD, Kwak D. Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea.. Pathogens 2020 Feb 11;9(2).
    doi: 10.3390/pathogens9020110pmc: PMC7168308pubmed: 32053974google scholar: lookup
  24. Shakya AK, O'Callaghan DJ, Kim SK. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11.. Front Vet Sci 2017;4:211.
    doi: 10.3389/fvets.2017.00211pmc: PMC5732242pubmed: 29312962google scholar: lookup
  25. OIE (World Organization for Animal Health). OIE Terrestrial Manual. OIE; Paris, France: 2018. Chapter 3.5.9: Equine rhinopneumonitis (infection with equid herpesvirus-1 and -4). .
  26. Ko S., Kang J.-G., Yeh J.-Y., Moon J.-S., Choi G.-C., Won S., Chae J.-S.. First Report on Molecular Detection of Equine Upper Respiratory Infectious Viruses in Republic of Korea.. J. Equine Vet. Sci. 2013;33:628–636.
    doi: 10.1016/j.jevs.2012.11.001google scholar: lookup
  27. Tsujimura K, Oyama T, Katayama Y, Muranaka M, Bannai H, Nemoto M, Yamanaka T, Kondo T, Kato M, Matsumura T. Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan.. J Vet Med Sci 2011 Dec;73(12):1663-7.
    doi: 10.1292/jvms.11-0140pubmed: 21828961google scholar: lookup
  28. Matczuk AK, Skarbek M, Jackulak NA, Bażanów BA. Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland.. Virol J 2018 Dec 3;15(1):186.
    doi: 10.1186/s12985-018-1093-5pmc: PMC6276253pubmed: 30509297google scholar: lookup
  29. Pronost S, Cook RF, Fortier G, Timoney PJ, Balasuriya UB. Relationship between equine herpesvirus-1 myeloencephalopathy and viral genotype.. Equine Vet J 2010 Nov;42(8):672-4.
    doi: 10.1111/j.2042-3306.2010.00307.xpubmed: 21039794google scholar: lookup
  30. Pusterla N, Hatch K, Crossley B, Wademan C, Barnum S, Flynn K. Equine herpesvirus-1 genotype did not significantly affect clinical signs and disease outcome in 65 horses diagnosed with equine herpesvirus-1 myeloencephalopathy.. Vet J 2020 Jan;255:105407.
    doi: 10.1016/j.tvjl.2019.105407pubmed: 31982080google scholar: lookup
  31. Vissani MA, Becerra ML, Olguín Perglione C, Tordoya MS, Miño S, Barrandeguy M. Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina.. Vet Microbiol 2009 Nov 18;139(3-4):361-4.
    doi: 10.1016/j.vetmic.2009.06.025pubmed: 19589651google scholar: lookup
  32. Vaz PK, Horsington J, Hartley CA, Browning GF, Ficorilli NP, Studdert MJ, Gilkerson JR, Devlin JM. Evidence of widespread natural recombination among field isolates of equine herpesvirus 4 but not among field isolates of equine herpesvirus 1.. J Gen Virol 2016 Mar;97(3):747-755.
    doi: 10.1099/jgv.0.000378pmc: PMC5381393pubmed: 26691326google scholar: lookup
  33. Gryspeerdt A., Vandekerckhove A., Van Doorsselaere J., Van de Walle G., Nauwynck H.. Description of an unusually large outbreak of nervous system disorders caused by equine herpesvirus 1 (EHV1) in 2009 in Belgium.. Vlaams Diergeneeskd. Tijdschr. 2011;80:147–153.
  34. Barbić L, Lojkić I, Stevanović V, Bedeković T, Starešina V, Lemo N, Lojkić M, Madić J. Two outbreaks of neuropathogenic equine herpesvirus type 1 with breed-dependent clinical signs.. Vet Rec 2012 Mar 3;170(9):227.
    doi: 10.1136/vr.100150pubmed: 22262701google scholar: lookup
  35. Damiani AM, de Vries M, Reimers G, Winkler S, Osterrieder N. A severe equine herpesvirus type 1 (EHV-1) abortion outbreak caused by a neuropathogenic strain at a breeding farm in northern Germany.. Vet Microbiol 2014 Aug 27;172(3-4):555-62.
    doi: 10.1016/j.vetmic.2014.06.023pubmed: 25042527google scholar: lookup
  36. Guo X, Izume S, Okada A, Ohya K, Kimura T, Fukushi H. Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson's gazelle.. J Vet Med Sci 2014 Sep;76(9):1309-12.
    doi: 10.1292/jvms.14-0183pmc: PMC4197165pubmed: 24920546google scholar: lookup
  37. Said A, Damiani A, Osterrieder N. Ubiquitination and degradation of the ORF34 gene product of equine herpesvirus type 1 (EHV-1) at late times of infection.. Virology 2014 Jul;460-461:11-22.
    doi: 10.1016/j.virol.2014.05.009pubmed: 25010266google scholar: lookup
  38. Kirisawa R, Ui S, Takahashi A, Kawakami Y, Iwai H. Comparison of the genomes of attenuated equine herpesvirus-1 strains with their parent virulent strain.. Virology 1994 May 1;200(2):651-60.
    doi: 10.1006/viro.1994.1228pubmed: 7909970google scholar: lookup
  39. Pagamjav O, Sakata T, Matsumura T, Yamaguchi T, Fukushi H. Natural recombinant between equine herpesviruses 1 and 4 in the ICP4 gene.. Microbiol Immunol 2005;49(2):167-79.
    doi: 10.1111/j.1348-0421.2005.tb03716.xpubmed: 15722602google scholar: lookup

Citations

This article has been cited 2 times.
  1. Hu Y, Jia Q, Liu J, Sun W, Bao Z, Che C, Wu G, Fan B, Jarhen, Ran D. Molecular characteristics and pathogenicity of an equid alphaherpesvirus 1 strain isolated in China. Virus Genes 2022 Aug;58(4):284-293.
    doi: 10.1007/s11262-022-01910-ypubmed: 35567668google scholar: lookup
  2. Petano-Duque JM, Urueña-Martinez E, Cabezas-Callejas LL, Perilla-Amaya J, Rueda-García V, Rondón-Barragán IS, Lopera-Vásquez R. Molecular and Serological Investigation of Equine Herpesvirus Type 1 (EHV-1) and Type 4 (EHV-4) in Horses In Ibagué, Tolima. Vet Med Int 2025;2025:1661949.
    doi: 10.1155/vmi/1661949pubmed: 39949613google scholar: lookup
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