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Pathogens (Basel, Switzerland)2021; 10(4); 425; doi: 10.3390/pathogens10040425

Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea.

Abstract: Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7-100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701-818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.
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Publication Date: 2021-04-02 PubMed ID: 33918404PubMed Central: PMC8066002DOI: 10.3390/pathogens10040425Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to understand the genetic makeup of the Equine herpesvirus-1 (EHV-1), a common horse pathogen in South Korea, by comparing isolated samples with existing genetic sequences. The study found that all the isolated viruses were of a non-neuropathogenic type and were classified into two groups based on ORF34 and multi-locus sequence typing (MLST) analysis.

Objective and Methodology

  • The researchers aimed to understand the genetic variability of the equine herpesvirus-1 (EHV-1) found in South Korea. This was done by collecting samples from 338 horses, out of which 12 EHV-1 were isolated.
  • The genetic makeup of these isolates was then compared to existing sequence data using the ORF30, ORF33, ORF68, and ORF34 genes and multi-locus sequence typing (MLST).

Findings

  • The study confirmed that all isolated viruses belonged to the non-neuropathogenic type, indicated by N752 of the ORF30 gene and a highly conserved ORF33 gene (99.7-100%). This type doesn’t cause damage to the nervous system of the horse.
  • The researchers observed a 118 base pair (bp) deletion in the nucleotide sequences 701-818 of the ORF68 gene which made it impossible to classify the isolates using ORF68 analysis. Sequence deletions like this can often disrupt the functioning of the resulting protein and could be a significant factor in the pathogenicity or virulence of the virus.
  • On analysis of the ORF34 gene and multi-locus sequence typing (MLST), the EHV-1 isolates were classified into two groups: one group comprised of seven isolates, falling under group 1, clade 10, and the other group included the remaining five isolates which fell under group 7, clade 6. This kind of grouping allows scientists to understand transmission patterns and evolutionary relationships between different virus isolates.

Significance

  • The research provides valuable insights into the genetic makeup of the EHV-1 virus in South Korea, thus improving the understanding of this widespread horse pathogen.
  • Finding sequence variability and groupings assist in tracking virus evolution and transmission patterns, information that can be pivotal in controlling the incidence and spread of the disease in equine populations.
  • Identification of a non-neuropathogenic type of EHV-1 adds to the understanding of the kind of EHV-1 strains predominant in South Korean horses, providing key details that can guide treatment and prevention strategies.

Cite This Article

APA
Kang HW, Lee EY, Lee KK, Ko MK, Park JY, Kim YH, Lee K, Choi EJ, Kim J, So B, Park CK, Jeoung HY. (2021). Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea. Pathogens, 10(4), 425. https://doi.org/10.3390/pathogens10040425

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 4
PII: 425

Researcher Affiliations

Kang, Hyung-Woo
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
  • College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Korea.
Lee, Eun-Yong
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Lee, Kyoung-Ki
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Ko, Mi-Kyeong
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Park, Ji-Young
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Kim, Yeon-Hee
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Lee, Kyunghyun
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Choi, Eun-Jin
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Kim, Jongho
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
So, ByungJae
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.
Park, Choi-Kyu
  • College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Korea.
Jeoung, Hye-Young
  • Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si 39660, Korea.

Grant Funding

  • B-1543069-2019-20-03 / Animal and Plant Quarantine Agency

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
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