Evaluation of tissue adhesive to contain axonal regeneration in horses.
Abstract: Bilateral palmar and plantar digital neurectomies were completed in 10 horses (a total of 80 neurectomies) using one of three methods: (1) simple transection (guillotine method); (2) epineural capping; (3) n-butyl cyanoacrylate injected into the epineural sheath to act as a nerve sealant. Horses were regularly evaluated clinically for tenderness in and around the surgical site, as well as skin sensation at the coronary band in the heel region, during the 12-week course of the study. None of the surgical sites exhibited any signs of drainage or infection. Horses were then euthanatized, the nerve stumps were dissected from surrounding tissues, and the length and width of the tissue mass that had formed on the end of the nerve was recorded. Longitudinal and transverse sections of the nerve endings were examined histologically for numbers of proliferating axon sprouts (neuroma formation); whether the axons had penetrated the epineurium; degree of Schwann cell proliferation; degree of chronic inflammation; extent of foreign body reaction; extent of retrograde degeneration of the nerve bundles; and amount of fibrovascular proliferation. The proportion of legs exhibiting tenderness or heel sensation did not differ significantly between the three different treatments at any of the six different times they were examined. There was no difference between the three treatments in the length or width of the fibrous tissue scar on the ends of the nerves or in the number of sprouting axons from the ends of the nerves. Of 80 nerves examined, only two nerves were not confined to the epineurium.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1995-07-01 PubMed ID: 7571382DOI: 10.1111/j.1532-950x.1995.tb01335.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study sought to evaluate the effectiveness of a tissue adhesive, n-butyl cyanoacrylate, in containing axonal regeneration in horses, in comparison to two other methods. The findings suggested that there were no significant differences between the three treatments in terms of healing tendency and post-surgery complications.
Methods and Procedure
- The authors carried out bilateral palmar and plantar digital neurectomies (nerve removals) on 10 horses, totaling 80 neurectomies.
- The neurectomies were performed using one of three methods: a simple transection, epineural capping, and injection of n-butyl cyanoacrylate into the epineural sheath to serve as a nerve sealant.
Observation and Assessment
- During the 12-week study period, the horses were regularly assessed for any tenderness around the surgical site and skin sensation at the coronary band in the heel region.
- At the end of the study, the nerve stumps were dissected, and the tissue that had formed on the nerve was measured. Additionally, longitudinal and transverse sections of the nerve endings were studied histologically to examine numerous factors including formation of new axon sprouts (neuromas), degree of Schwann cell proliferation, chronic inflammation, foreign body reaction, retrograde degeneration of nerve bundles, and extent of fibrovascular proliferation.
Results
- No significant drainage or infection was observed at any of the surgical sites over the course of the study.
- The results indicated no significant differences between the three treatments regarding the proportion of legs that displayed tenderness or heel sensation at any of the examined times.
- Furthermore, there were no differences among the three methods concerning the length or width of the fibrous tissue scar on the nerve ends or in the number of sprouting axons from the nerve ends.
Conclusion
- Of the 80 nerves studied, only two did not stay within the epineurium, indicating a high rate of containment for all three methods. Overall, the initial results suggest that there is not a significant difference between using the tissue adhesive and the other two methods for neurectomy in horses regarding healing tendencies and post-surgery complications.
Cite This Article
APA
Turner AS, Trotter GW, Powers BE.
(1995).
Evaluation of tissue adhesive to contain axonal regeneration in horses.
Vet Surg, 24(4), 308-314.
https://doi.org/10.1111/j.1532-950x.1995.tb01335.x Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, USA.
MeSH Terms
- Animals
- Axons / drug effects
- Axons / pathology
- Axons / physiology
- Drug Evaluation
- Enbucrilate / analogs & derivatives
- Enbucrilate / pharmacology
- Foot / innervation
- Foot / surgery
- Forelimb / innervation
- Forelimb / surgery
- Hindlimb / innervation
- Hindlimb / surgery
- Horses / surgery
- Neoplasms, Nerve Tissue / prevention & control
- Nerve Regeneration / drug effects
- Neuroma / prevention & control
- Neuroma / veterinary
- Peripheral Nerves / drug effects
- Peripheral Nerves / pathology
- Peripheral Nerves / physiology
- Peripheral Nerves / surgery
- Postoperative Care / veterinary
- Tissue Adhesives / pharmacology
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