Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus.
Abstract: In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity.
Publication Date: 2018-12-18 PubMed ID: 30607135PubMed Central: PMC6306293DOI: 10.1294/jes.29.111Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Antibodies
- Clinical Study
- Comparative Study
- Diagnostic Technique
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Viral Arteritis
- Horses
- Immunology
- Infection
- Infectious Disease
- Laboratory Methods
- Sensitivity and Specificity
- Serodiagnosis
- Serology
- Specificity
- Veterinary Medicine
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article discusses a comparison study of two Enzyme-Linked Immunosorbent Assays (ELISAs), produced by different manufacturers, for their efficacy in the detection of antibodies against the equine arteritis virus. The comparable sensitivity but notably different specificity rates of the two assays were found, with the VMRD-ELISA showing superior performance.
Objective of Research
- This study aims to establish an effective system for detecting the equine arteritis virus in Japan.
- To achieve this, the research compares two ELISAs used to detect antibodies against the equine arteritis virus.
- The two ELISAs in question are produced by Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, USA.
Methods Used
- The researchers tested a number of horse serum samples using both the Nisseiken and VMRD ELISAs.
- The number of samples that tested positive to Virus-Neutralizing (VN) antibodies in each ELISA were analyzed and recorded.
Results
- Out of 160 VN antibody-positive serum samples, 159 tested positive in both the Nisseiken-ELISA and VMRD-ELISA.
- Of the VN antibody-negative sera, 134 samples were negative in the Nisseiken-ELISA while a larger number (154) were negative in the VMRD-ELISA.
- Thus, both ELISAs demonstrated a nearly perfect sensitivity rate of 99.4%.
Interpretation of Findings
- The specificity of the two ELISAs varied significantly. The VMRD-ELISA demonstrated higher specificity at 98.1%, compared to the Nisseiken-ELISA, which only had a specificity rate of 85.4%.
- The superior specificity of the VMRD-ELISA made it a more reliable diagnostic tool for detecting the equine arteritis virus.
- The higher specificity of the VMRD-ELISA means it has a lower chance of giving false-positive results compared to the Nisseiken-ELISA.
Conclusion
- Although both the Nisseiken and VMRD ELISAs demonstrated high sensitivity in detecting antibodies against the equine arteritis virus, the VMRD-ELISA had significantly higher specificity.
- This makes the VMRD-ELISA more reliable in serological diagnosis, as it has a lower probability of giving false-positive results.
- As such, the VMRD-ELISA could potentially offer a more reliable tool for serological diagnosis in Japan.
Cite This Article
APA
Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T.
(2018).
Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus.
J Equine Sci, 29(4), 111-115.
https://doi.org/10.1294/jes.29.111 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
References
This article includes 15 references
- Balasuriya U.B.. Equine viral arteritis.. Vet. Clin. North Am. Equine Pract. 30: 543–560.
- Chirnside E.D., Francis P.M., de Vries A.A., Sinclair R., Mumford J.A.. Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus.. J. Virol. Methods 54: 1–13.
- Cho H.J., Entz S.C., Deregt D., Jordan L.T., Timoney P.J., McCollum W.H.. Detection of antibodies to equine arteritis virus by a monoclonal antibody-based blocking ELISA.. Can. J. Vet. Res. 64: 38–43.
- Chung C., Wilson C., Timoney P., Adams E., Adams D.S., Chung J.S., Evermann J.F., Shuck K., Lee S.S., McGuire T.C.. Comparison of an improved competitive enzyme-linked immunosorbent assay with the World Organization for Animal Health-prescribed serum neutralization assay for detection of antibody to equine arteritis virus.. J. Vet. Diagn. Invest. 25: 182–188.
- Duthie S., Mills H., Burr P.. The efficacy of a commercial ELISA as an alternative to virus neutralisation test for the detection of antibodies to EAV.. Equine Vet. J. 40: 182–183.
- Kondo T., Fukunaga Y., Sekiguchi K., Sugiura T., Imagawa H.. Enzyme-linked immunosorbent assay for serological survey of equine arteritis virus in racehorses.. J. Vet. Med. Sci. 60: 1043–1045.
- Legrand L., Pitel P.H., Fortier G., Pronost S., Cullinane A.. Testing for antibodies to equine arteritis virus.. Vet. Rec. 164: 437.
- MacLachlan N.J.. Laboratory diagnosis of viral infections.. pp.101–123. In: Fenner’s Veterinary Virology, 4th ed. (MacLachlan, N. J. and Dubovi, E. J. eds.), Academic Press, London..
- Newton J.R., Geraghty R.J., Castillo-Olivares J., Cardwell J.M., Mumford J.A.. Evidence that use of an inactivated equine herpesvirus vaccine induces serum cytotoxicity affecting the equine arteritis virus neutralisation test.. Vaccine 22: 4117–4123.
- Nugent J., Sinclair R., deVries A.A., Eberhardt R.Y., Castillo-Olivares J., Davis Poynter N., Rottier P.J., Mumford J.A.. Development and evaluation of ELISA procedures to detect antibodies against the major envelope protein (GL) of equine arteritis virus.. J. Virol. Methods 90: 167–183.
- OIE. Equine Viral Arteritis.. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2013. Paris, France: World Organization for Animal Health..
- Pfahl K., Chung C., Singleton M.D., Shuck K.M., Go Y.Y., Zhang J., Campos J., Adams E., Adams D.S., Timoney P.J., Balasuriya U.B.. Further evaluation and validation of a commercially available competitive ELISA (cELISA) for the detection of antibodies specific to equine arteritis virus (EAV).. Vet. Rec. 178: 95.
- Timoney P.J., McCollum W.H.. Equine viral arteritis.. Vet. Clin. North Am. Equine Pract. 9: 295–309.
- Turan N., Ekici H., Yilmaz H., Kondo T., Hasoksuz M., Sato I., Tuchiya K., Fukunaga Y.. Detection of antibodies to equine arteritis virus in horse sera using recombinant chimaeric N/GL protein.. Vet. Rec. 161: 352–354.
- Wagner H.M., Balasuriya U.B., James MacLachlan N.. The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins.. Comp. Immunol. Microbiol. Infect. Dis. 26: 251–260.
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
