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Home / Equine Research Bank / Appl Environ Microbiol / Evaluation of virulence factor profiling in the characteriza...
Applied and environmental microbiology2010; 76(22); 7509-7513; doi: 10.1128/AEM.00726-10

Evaluation of virulence factor profiling in the characterization of veterinary Escherichia coli isolates.

Abstract: Escherichia coli has been used as an indicator organism for fecal contamination of water and other environments and is often a commensal organism in healthy animals, yet a number of strains can cause disease in young or immunocompromised animals. In this study, 281 E. coli isolates from bovine, porcine, chicken, canine, equine, feline, and other veterinary sources were analyzed by BOXA1R PCR and by virulence factor profiling of 35 factors to determine whether they had utility in identifying the animal source of the isolates. The results of BOXA1R PCR analysis demonstrated a high degree of diversity; less than half of the isolates fell into one of 27 clusters with at least three isolates (based on 90% similarity). Nearly 60% of these clusters contained isolates from more than one animal source. Conversely, the results of virulence factor profiling demonstrated clustering by animal source. Three clusters, named Bovine, Chicken, and Porcine, based on discriminant components analysis, were represented by 90% or more of the respective isolates. A fourth group, termed Companion, was the most diverse, containing at least 84% of isolates from canine, feline, equine, and other animal sources. Based on these results, it appears that virulence factor profiling may have utility, helping identify the likely animal host species sources of certain E. coli isolates.
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Publication Date: 2010-10-01 PubMed ID: 20889790PubMed Central: PMC2976202DOI: 10.1128/AEM.00726-10Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates whether the profiling of virulence factors, traits that increase a microorganism’s capacity to cause disease, can help identify the animal source of Escherichia coli bacteria strains. The study demonstrated that this profiling technique may be useful in identifying the likely animal host species of certain E. coli strains.

Study Design and Methodology

  • The research examined 281 E. coli isolates sourced from various animals such as bovines, canines, horses, chickens, cats, pigs and others.
  • Two methods were used to analyze these isolates: BOXA1R PCR, a method of identifying genetic differences, and virulence factor profiling, which looks at 35 factors that potentially contribute to a bacterium’s ability to cause disease.
  • The aim was to discover whether these methods could identify the animal source of different E. coli isolates.

Results of BOXA1R PCR Analysis

  • BOXA1R PCR analysis revealed a high degree of genetic diversity among the isolates.
  • Less than half of the isolates formed one of 27 clusters based on at least 90% genetic similarity. This indicates a wide genetic variation in the E. coli isolates.
  • Interestingly, nearly 60% of the formed clusters contained isolates from more than one animal source, signifying cross-species transmission.

Results of Virulence Factor Profiling

  • On the other hand, virulence factor profiling demonstrated a greater correlation to the animal source of the isolates.
  • Three clusters, named Bovine, Chicken, and Porcine, were found, with 90% or more of the isolates in each cluster coming from the respective animal species.
  • A fourth group named “Companion” was the most diverse, containing at least 84% of isolates from animals like dogs, cats, horses, and others.

Conclusions

  • The study concluded that while BOXA1R PCR revealed genetic diversity among isolates, virulence factor profiling provided more useful results in identifying the likely animal host species sources of E. coli isolates.
  • This suggests that virulence factor profiling can potentially be utilized as a tool for tracing the source of certain E. coli infections in veterinary medicine.

Cite This Article

APA
David DE, Lynne AM, Han J, Foley SL. (2010). Evaluation of virulence factor profiling in the characterization of veterinary Escherichia coli isolates. Appl Environ Microbiol, 76(22), 7509-7513. https://doi.org/10.1128/AEM.00726-10

Publication

Applied and environmental microbiology
ISSN: 1098-5336
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 76
Issue: 22
Pages: 7509-7513

Researcher Affiliations

David, Donna E
  • National Center for Toxicological Research, Jefferson, AR 72079, USA.
Lynne, Aaron M
    Han, Jing
      Foley, Steven L

        MeSH Terms

        • Animals
        • Animals, Domestic
        • Bacterial Typing Techniques
        • Bacteriological Techniques / methods
        • Cluster Analysis
        • Escherichia coli / genetics
        • Escherichia coli / isolation & purification
        • Escherichia coli / pathogenicity
        • Escherichia coli Infections / microbiology
        • Escherichia coli Infections / veterinary
        • Escherichia coli Proteins / genetics
        • Genetic Variation
        • Polymerase Chain Reaction / methods
        • Virulence Factors / genetics

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        Citations

        This article has been cited 2 times.
        1. Kaldhone PR, Carlton A, Aljahdali N, Khajanchi BK, Sanad YM, Han J, Deck J, Ricke SC, Foley SL. Evaluation of Incompatibility Group I1 (IncI1) Plasmid-Containing Salmonella enterica and Assessment of the Plasmids in Bacteriocin Production and Biofilm Development.. Front Vet Sci 2019;6:298.
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        2. Gokulan K, Khare S, Rooney AW, Han J, Lynne AM, Foley SL. Impact of plasmids, including those encodingVirB4/D4 type IV secretion systems, on Salmonella enterica serovar Heidelberg virulence in macrophages and epithelial cells.. PLoS One 2013;8(10):e77866.
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