FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Virol / Evidence for a relationship between equine abortion (herpes)...
Journal of virology1971; 7(6); 736-748; doi: 10.1128/JVI.7.6.736-748.1971

Evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the S phase of the KB cell mitotic cycle.

Abstract: Autoradiographic analyses of deoxyribonucleic acid (DNA) synthesis in randomly growing KB cell cultures infected with equine abortion virus (EAV) suggested that viral DNA synthesis was initiated only at times that coincided with the entry of noninfected control cells into the S phase of the cell cycle. Synchronized cultures of KB cells were infected at different stages of the cell cycle, and rates of synthesis of cellular and viral DNA were measured. When cells were infected at different times within the S phase, viral DNA synthesis was initiated 2 to 3 hr after infection. However, when cells in G1 and G2 were infected, the initiation of viral DNA synthesis was delayed and occurred only at times corresponding to the S phase. The times when viral DNA synthesis began were independent of the time of infection and differed by as much as 5 hr, depending on the stage of the cell cycle at which cells were infected. Viral one-step growth curves were also related to the S phase in a manner which indicated a relationship between the initiation of viral DNA synthesis and the S phase. These data support the concept that initiation of EAV DNA synthesis is dependent upon some cellular function(s) which is related to the S phase of the cell cycle.
Get Full Text
Publication Date: 1971-06-01 PubMed ID: 4254680PubMed Central: PMC356192DOI: 10.1128/JVI.7.6.736-748.1971Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the relationship between the synthesis of equine abortion virus (EAV) DNA and the S phase of the KB cell’s cycle, showing evidence that the initiation of EAV DNA synthesis occurs during the S phase likely due to certain cellular functions.

Background

  • The study undertakes a focused analysis of the relationship between the synthesis (creation) of the DNA of the equine abortion virus (EAV) and a specific phase of the KB cell cycle known as the S phase. The authors suggest that the initiation of viral DNA synthesis is directly related to the S phase of the cell cycle.

Methodology

  • The authors used autoradiographic techniques to analyze DNA synthesis in growing KB cell cultures infected with EAV.
  • The research involved infecting synchronized cultures of KB cells at different stages of the cell cycle and measuring the rates of synthesis of both cellular and viral DNA.
  • The cells were infected during different times within the S phase and at stages G1 and G2 of the cell cycle.

Findings

  • Critical to the findings was that the initiation of viral DNA synthesis was linked to the S phase of the cell cycle. If the cells were infected during the S phase, viral DNA synthesis began 2-3 hours after infection.
  • If the cells were infected during the G1 and G2 phases, the viral DNA synthesis was delayed and only occurred during times corresponding with the S phase.
  • The time at which viral DNA synthesis began was found to be independent of the infection time and differed by as much as 5 hours, depending on the stage of the cell cycle during which the cells were infected.
  • In addition, data showed that viral one-step growth curves were also related to the S phase. This indicates a relationship between the initiation of viral DNA synthesis and the S phase.

Conclusions

  • The research provides evidence to support the concept that the initiation of the EAV DNA synthesis relies on certain cellular functions that are associated with the S phase of the cell cycle.

Cite This Article

APA
Lawrence WC. (1971). Evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the S phase of the KB cell mitotic cycle. J Virol, 7(6), 736-748. https://doi.org/10.1128/JVI.7.6.736-748.1971

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 7
Issue: 6
Pages: 736-748

Researcher Affiliations

Lawrence, W C

    MeSH Terms

    • Animals
    • Autoradiography
    • Carbon Isotopes
    • Carcinoma
    • Cell Line
    • Centrifugation, Density Gradient
    • Cesium
    • Chlorides
    • Culture Media
    • Culture Techniques
    • DNA / biosynthesis
    • DNA / isolation & purification
    • DNA, Viral / biosynthesis
    • DNA, Viral / isolation & purification
    • Floxuridine / pharmacology
    • Herpesviridae / growth & development
    • Herpesviridae / metabolism
    • Herpesviridae / pathogenicity
    • Horse Diseases / microbiology
    • Horses
    • Humans
    • Hypoxanthines / metabolism
    • Mitosis
    • Mouth Neoplasms
    • Thymidine / metabolism
    • Time Factors
    • Tritium
    • Virus Replication

    References

    This article includes 34 references
    1. Bello LJ. Synthesis of DNA-like RNA in synchronized cultures of mammalian cells.. Biochim Biophys Acta 1968 Mar 18;157(1):8-15.
      pubmed: 5642645doi: 10.1016/0005-2787(68)90258-xgoogle scholar: lookup
    2. DARLINGTON RW, RANDALL CC. The nucleic acid content of equine abortion virus.. Virology 1963 Mar;19:322-7.
      pubmed: 14025123doi: 10.1016/0042-6822(63)90071-0google scholar: lookup
    3. O'Callaghan DJ, Hyde JM, Gentry GA, Randall CC. Kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells.. J Virol 1968 Aug;2(8):793-804.
      pubmed: 4302745doi: 10.1128/JVI.2.8.793-804.1968google scholar: lookup
    4. Groyon RM, Kniazeff AJ. Vaccinia virus infection of synchronized pig kidney cells.. J Virol 1967 Dec;1(6):1255-64.
      pubmed: 4192871doi: 10.1128/JVI.1.6.1255-1264.1967google scholar: lookup
    5. SALZMAN NP. The rate of formation of vaccinia deoxyribonucleic acid and vaccinia virus.. Virology 1960 Jan;10:150-2.
      pubmed: 14441184doi: 10.1016/0042-6822(60)90015-5google scholar: lookup
    6. Lawrence WC. Nucleic acid and protein synthesis in KB cells infected with equine abortion virus (equine herpesvirus type 1).. Am J Vet Res 1971 Jan;32(1):41-9.
      pubmed: 5099956
    7. Randall CC, Gafford LG, Gentry GA, Lawson LA. Lability of host-cell DNA in growing cell cultures due to Mycoplasma.. Science 1965 Sep 3;149(3688):1098-9.
      pubmed: 5826522doi: 10.1126/science.149.3688.1098google scholar: lookup
    8. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.. Proc Soc Exp Biol Med 1955 Jul;89(3):362-4.
      pubmed: 13254761doi: 10.3181/00379727-89-21811google scholar: lookup
    9. ROBBINS E, MARCUS PI. MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS.. Science 1964 May 29;144(3622):1152-3.
      pubmed: 14148440doi: 10.1126/science.144.3622.1152google scholar: lookup
    10. O'Callaghan DJ, Cheevers WP, Gentry GA, Randall CC. Kinetics of cellular and viral DNA synthesis in equine abortion (herpes) virus infection of L-M cells.. Virology 1968 Sep;36(1):104-14.
      pubmed: 5669981doi: 10.1016/0042-6822(68)90120-7google scholar: lookup
    11. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochem J 1956 Feb;62(2):315-23.
      pubmed: 13293190doi: 10.1042/bj0620315google scholar: lookup
    12. XEROS N. Deoxyriboside control and synchronization of mitosis.. Nature 1962 May 19;194:682-3.
      pubmed: 14008663doi: 10.1038/194682a0google scholar: lookup
    13. SALZMAN NP, SHATKIN AJ, SEBRING ED, MUNYON W. On the replication of vaccinia virus.. Cold Spring Harb Symp Quant Biol 1962;27:237-44.
      pubmed: 13991264doi: 10.1101/sqb.1962.027.001.023google scholar: lookup
    14. TERASIMA T, TOLMACH LJ. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells.. Exp Cell Res 1963 Apr;30:344-62.
      pubmed: 13980637doi: 10.1016/0014-4827(63)90306-9google scholar: lookup
    15. KAPLAN AS, BEN-PORAT T. The effect of pseudorabies virus on the nucleic acid metabolism and on the nuclei of rabbit kidney cells.. Virology 1959 Jul;8(3):352-66.
      pubmed: 13669354doi: 10.1016/0042-6822(59)90035-2google scholar: lookup
    16. Tennant RW, Layman KR, Hand RE. Effect of cell physiological state on infection by rat virus.. J Virol 1969 Dec;4(6):872-8.
      pubmed: 16789120doi: 10.1128/JVI.4.6.872-878.1969google scholar: lookup
    17. BOOTSMA D, BUDKE L, VOS O. STUDIES ON SYNCHRONOUS DIVISION OF TISSUE CULTURE CELLS INITIATED BY EXCESS THYMIDINE.. Exp Cell Res 1964 Jan;33:301-9.
      pubmed: 14109144doi: 10.1016/s0014-4827(64)81035-1google scholar: lookup
    18. GREEN M. Studies on the biosynthesis of viral DNA.. Cold Spring Harb Symp Quant Biol 1962;27:219-35.
      pubmed: 13950095doi: 10.1101/sqb.1962.027.001.022google scholar: lookup
    19. Pfeiffer SE, Tolmach LJ. Selecting synchronous populations of mammalian cells.. Nature 1967 Jan 14;213(5072):139-42.
      pubmed: 6030567doi: 10.1038/213139a0google scholar: lookup
    20. Kaplan AS, Ben-Porat T. Metabolism of animal cells infected with nuclear DNA viruses.. Annu Rev Microbiol 1968;22:427-50.
      pubmed: 4301616doi: 10.1146/annurev.mi.22.100168.002235google scholar: lookup
    21. Tennant RW, Hand RE Jr. Requirement of cellular synthesis for Kilham rat virus replication.. Virology 1970 Dec;42(4):1054-63.
      pubmed: 4992351doi: 10.1016/0042-6822(70)90353-3google scholar: lookup
    22. Kroon AM, Borst P, Bruggen EF, Ruttenberg GJ. Mitochondrial DNA from sheep heart.. Proc Natl Acad Sci U S A 1966 Dec;56(6):1836-43.
      pubmed: 16591428doi: 10.1073/pnas.56.6.1836google scholar: lookup
    23. CAIRNS J. The initiation of vaccinia infection.. Virology 1960 Jul;11:603-23.
      pubmed: 13806834doi: 10.1016/0042-6822(60)90103-3google scholar: lookup
    24. LOCKART RZ Jr, EAGLE H. Requirements for growth of single human cells.. Science 1959 Jan 30;129(3344):252-4.
      pubmed: 13624712doi: 10.1126/science.129.3344.252google scholar: lookup
    25. KAPLAN AS. Analysis of the intracellular development of a DNA-containing mammalian virus (pseudorabies) by means of ultraviolet light irradiation.. Virology 1962 Mar;16:305-13.
      pubmed: 14453720doi: 10.1016/0042-6822(62)90251-9google scholar: lookup
    26. Lawrence WC, Ginsberg HS. Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid.. J Virol 1967 Oct;1(5):851-67.
      pubmed: 5621482doi: 10.1128/JVI.1.5.851-867.1967google scholar: lookup
    27. Hodge LD, Scharff MD. Effect of adenovirus on host cell DNA synthesis in synchronized cells.. Virology 1969 Apr;37(4):554-64.
      pubmed: 5781860doi: 10.1016/0042-6822(69)90273-6google scholar: lookup
    28. Galavazi G, Bootsma D. Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. II. Population dynamics.. Exp Cell Res 1966 Feb;41(2):438-51.
      pubmed: 5929557doi: 10.1016/s0014-4827(66)80150-7google scholar: lookup
    29. Darlington RW, James C. Biological and morphological aspects of the growth of equine abortion virus.. J Bacteriol 1966 Jul;92(1):250-7.
      pubmed: 5941279doi: 10.1128/jb.92.1.250-257.1966google scholar: lookup
    30. Hampton EG. H-1 virus growth in synchronized rat embryo cells.. Can J Microbiol 1970 Apr;16(4):266-8.
      pubmed: 5462976doi: 10.1139/m70-049google scholar: lookup
    31. SOEHNER RL, GENTRY GA, RANDALL CC. SOME PHYSICOCHEMICAL CHARACTERISTICS OF EQUINE ABORTION VIRUS NUCLEIC ACID.. Virology 1965 Jul;26:394-405.
      pubmed: 14319712doi: 10.1016/0042-6822(65)90003-6google scholar: lookup
    32. Gershon D, Sachs L, Winocour E. The induction of cellular DNA synthesis by simian virus 40 in contact-inhibited and in x-irradiated cells.. Proc Natl Acad Sci U S A 1966 Sep;56(3):918-25.
      pubmed: 16578647doi: 10.1073/pnas.56.3.918google scholar: lookup
    33. Shimojo H, Yamashita T. Induction of DNA synthesis by adenoviruses in contact-inhibited hamster cells.. Virology 1968 Nov;36(3):422-33.
      pubmed: 5722184doi: 10.1016/0042-6822(68)90167-0google scholar: lookup
    34. Puck TT. Phasing, Mitotic Delay, and Chromosomal Aberrations in Mammalian Cells.. Science 1964 May 1;144(3618):565-6.
      pubmed: 17836382doi: 10.1126/science.144.3618.565-cgoogle scholar: lookup

    Citations

    This article has been cited 11 times.
    1. Rhode SL 3rd. Replication process of the parvovirus H-1. I. Kinetics in a parasynchronous cell system. J Virol 1973 Jun;11(6):856-61.
      doi: 10.1128/JVI.11.6.856-861.1973pubmed: 4736535google scholar: lookup
    2. Cohen GH, Factor MN, Ponce de Leon M. Inhibition of herpes simplex virus type 2 replication by thymidine. J Virol 1974 Jul;14(1):20-5.
      doi: 10.1128/JVI.14.1.20-25.1974pubmed: 4365718google scholar: lookup
    3. Pages J, Manteuil S, Stehelin D, Fiszman M, Marx M, Girard M. Relationship between replication of simian virus 40 DNA and specific events of the host cell cycle. J Virol 1973 Jul;12(1):99-107.
      doi: 10.1128/JVI.12.1.99-107.1973pubmed: 4353504google scholar: lookup
    4. Cohen GH. Ribonucleotide reductase activity of synchronized KB cells infected with herpes simplex virus. J Virol 1972 Mar;9(3):408-18.
      doi: 10.1128/JVI.9.3.408-418.1972pubmed: 4335659google scholar: lookup
    5. Cohen GH, Vaughan RK, Lawrence WC. Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus. J Virol 1971 Jun;7(6):783-91.
      doi: 10.1128/JVI.7.6.783-791.1971pubmed: 4327586google scholar: lookup
    6. Kit S. Thymidine kinase, DNA synthesis and cancer. Mol Cell Biochem 1976 Jun 15;11(3):161-82.
      doi: 10.1007/BF01744997pubmed: 940549google scholar: lookup
    7. Kisary J. Interaction in replication between goose parvovirus strain B and duck plague herpesvirus. Arch Virol 1979;59(1-2):81-8.
      doi: 10.1007/BF01317897pubmed: 426633google scholar: lookup
    8. Kraft V, Tischer I. Cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts. Arch Virol 1978;57(3):243-54.
      doi: 10.1007/BF01315088pubmed: 209769google scholar: lookup
    9. Muller MT, Hudson JB. Cell cycle dependency of murine cytomegalovirus replication in synchronized 3T3 cells. J Virol 1977 May;22(2):267-72.
      doi: 10.1128/JVI.22.2.267-272.1977pubmed: 194054google scholar: lookup
    10. DeMarchi JM, Kaplan AS. Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. J Virol 1976 Jun;18(3):1063-70.
      doi: 10.1128/JVI.18.3.1063-1070.1976pubmed: 178912google scholar: lookup
    11. Estes JE, Huang ES. Stimulation of cellular thymidine kinases by human cytomegalovirus. J Virol 1977 Oct;24(1):13-21.
      doi: 10.1128/JVI.24.1.13-21.1977pubmed: 20515google scholar: lookup
    Subscribe to our newsletter
    Join 99,001 horse owners like you who receive our email updates on horse health and nutrition.