Evidence of a second polymorphic ELA class I (ELA-B) locus and gene order for three loci of the equine major histocompatibility complex.
Abstract: Two antisera, B-442 and R-2046, were produced by immunizing offspring with purified peripheral blood lymphocytes from a parent matched for the ELA-A specificity carried on the unshared haplotype. Absorption analysis demonstrated that these antisera contained at least two families of cytotoxic antibodies, one directed against antigens present on T and B cells, and a second directed preferentially against antigens present on surface Ig positive cells. Immunoprecipitation studies using these antisera demonstrated that both antisera contain antibodies specific for glycoproteins with molecular weights characteristic of class I and class II MHC antigens. In lymphocyte typing tests of unfractionated lymphocytes, only the class I activity was readily detectable since the class II activity killed less than 25% of the cells. Family studies demonstrated that these antisera recognize products of genes linked to the ELA system. Based on two recombinants in an extended family it became apparent that the specificities detected by B-442 and R-2046 are not products of the ELA-A locus, but rather they are products of at least one other locus, defined in this paper as ELA-B. In this family a third recombinant was found between the A blood group system and the ELA-A locus. Based on these three recombinants, the most probable linear relationship of the following genes is: A blood group system/ELA-A/ELA-B.
Publication Date: 1987-01-01 PubMed ID: 2959176DOI: 10.1111/j.1365-2052.1987.tb00749.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article explores the discovery of a second polymorphic ELA class I (ELA-B) locus and determines the gene order for three loci of the equine major histocompatibility complex. Their studies indicate that the antibodies produced by these antisera recognize the products of genes linked to the ELA system.
Immunization and Antisera Production
- The researchers made use of two antisera, called B-442 and R-2046. These were produced by immunizing an offspring using purified peripheral blood lymphocytes from a parent that matched for ELA-A specificity.
- Absorption analysis revealed that these antisera contained two types of cytotoxic antibodies: one targeted antigens present on T and B cells, and the other was more directed against antigens on surface Ig positive cells.
Immunoprecipitation Studies
- Immunoprecipitation studies were conducted using the previously mentioned antisera. Both antisera were observed to have antibodies specific for glycoproteins that share similar molecular weights with class I and class II MHC antigens.
- In tests that were conducted with unfractionated lymphocytes, only the class I activity was easily detectable, while the class II activity resulted in less than 25% cell death.
Linkage to the ELA System
- Further family studies showed that the antisera recognize the products of genes connected to the ELA system.
- The specificities detected by B-442 and R-2046 are not products of the ELA-A locus per their findings. Instead, these appear to be products of a different locus, which the researchers define in the paper as ELA-B.
Determining Gene Order
- Through studying two recombinants in a large family, evidence emerged that supports the presence of the ELA-B locus.
- Additionally, within this family, a third recombinant was identified between the A blood group system and the ELA-A locus.
- Based on these three recombinants, the most probable linear relationship of the genes is determined to be A blood group system, ELA-A, and ELA-B in that order.
Cite This Article
APA
Bernoco D, Byrns G, Bailey E, Lew AM.
(1987).
Evidence of a second polymorphic ELA class I (ELA-B) locus and gene order for three loci of the equine major histocompatibility complex.
Anim Genet, 18(2), 103-118.
https://doi.org/10.1111/j.1365-2052.1987.tb00749.x Publication
Researcher Affiliations
- Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
MeSH Terms
- Animals
- Antigens, Surface / genetics
- B-Lymphocytes / immunology
- Gene Frequency
- Haplotypes
- Horses / immunology
- Immune Sera
- Lymphocyte Culture Test, Mixed
- Major Histocompatibility Complex
- T-Lymphocytes / immunology
Grant Funding
- HD-14487 / NICHD NIH HHS
Citations
This article has been cited 3 times.- Chung C, Mealey RH, McGuire TC. Evaluation of high functional avidity CTL to Gag epitope clusters in EIAV carrier horses. Virology 2005 Nov 25;342(2):228-39.
- Chung C, Mealey RH, McGuire TC. CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins. Virology 2004 Sep 15;327(1):144-54.
- Zhang W, Lonning SM, McGuire TC. Gag protein epitopes recognized by ELA-A-restricted cytotoxic T lymphocytes from horses with long-term equine infectious anemia virus infection. J Virol 1998 Dec;72(12):9612-20.
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