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Veterinary research2012; 43(1); 53; doi: 10.1186/1297-9716-43-53

Evidence of host adaptation in Lawsonia intracellularis infections.

Abstract: Lawsonia intracellularis is the causative agent of proliferative enteropathy, an endemic disease in pigs and an emerging concern in horses. Enterocyte hyperplasia is a common lesion in every case but there are differences regarding clinical and pathological presentations among affected species. We hypothesize that host susceptibility to L. intracellularis infection depends on the species of origin of the bacterial isolate. The objective of this study was to evaluate the susceptibilities of pigs and horses to L. intracellularis infection using either a porcine or an equine isolate. Methods: Twelve foals and eighteen pigs were equally divided into three groups and infected with either a porcine or an equine isolate (109L. Intracellularis/challenged animal), and a saline solution (negative control group). The animals were monitored regarding clinical signs, average of daily weight gain, fecal shedding of the bacteria by PCR and humoral serological response. Results: Foals infected with the equine isolate developed moderate to severe clinical signs and maintained a lower average of weight gain compared to control foals. Fecal quantitative PCR in equine isolate-infected foals revealed higher amounts of bacterial DNA associated with longer duration of shedding compared with porcine isolate-infected foals. All four foals infected with the equine isolate demonstrated higher IgG titers in the serum compared with porcine isolate-infected foals. In the pig trial, diarrhea and seroconversion were only observed in animals infected with the porcine isolate. Pathological changes typical of proliferative enteropathy were observed in the necropsied foal infected with equine isolate and in the two necropsied pigs infected with the porcine isolate. Conclusions: Evident clinical signs, longer periods of bacterial shedding and stronger serologic immune responses were observed in animals infected with species-specific isolates. These results show that host susceptibility is driven by the origin of the isolated L. intracellularis strain.
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Publication Date: 2012-06-20 PubMed ID: 22715937PubMed Central: PMC3443049DOI: 10.1186/1297-9716-43-53Google Scholar: Lookup
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Summary

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The research article investigates the host adaptation in Lawsonia intracellularis infections. The study provides evidence that the susceptibility of hosts (specifically pigs and horses) to L. intracellularis infection depends on whether the bacterial isolate comes from the same species.

Research Objective

The researchers aimed to examine the susceptibility of pigs and horses to L. intracellularis, by using either a porcine (pig) or an equine (horse) bacterial isolate. The study wanted to understand whether the species origin of the bacterial isolate influenced the host’s response to infection.

Methodology

  • Twelve horses and eighteen pigs were divided into three groups. Two groups were infected with either a pig or horse isolate of the bacteria, and the third group was administered a saline solution (control group).
  • The research team monitored clinical signs, daily weight gain average, bacteria fecal shedding through PCR testing, and humoral serological response.

Results

  • Horses infected with the horse-specific isolate showed moderate to severe clinical signs, experienced a lower average weight gain compared to control horses, and revealed a higher quantity of bacterial DNA with a longer duration of shedding.
  • All horses infected with the horse-specific isolate demonstrated higher IgG titers (an indication of the immune system’s response to infection) compared to those infected with the pig isolate.
  • For pigs, diarrhea and seroconversion (the time period during which a specific antibody develops and becomes detectable in the blood) occurred only in those animals infected with the pig isolate.
  • Post-mortem examinations showed that lesions typical of proliferative enteropathy (the disease caused by L. intracellularis) were observed only in animals infected with species-specific bacterial isolates.

Conclusions

The study provided evidence that there is a strong correlation between the origin of the bacterial isolate and its corresponding host’s susceptibility. This was demonstrated by more severe clinical signs, increased bacterial shedding duration, and stronger immune response observed in animals infected with species-specific isolates. These findings show that the susceptibilities of pigs and horses to L. intracellularis infection are driven by the species origin of the L. intracellularis strain.

Cite This Article

APA
Vannucci FA, Pusterla N, Mapes SM, Gebhart C. (2012). Evidence of host adaptation in Lawsonia intracellularis infections. Vet Res, 43(1), 53. https://doi.org/10.1186/1297-9716-43-53

Publication

Veterinary research
ISSN: 1297-9716
NlmUniqueID: 9309551
Country: England
Language: English
Volume: 43
Issue: 1
Pages: 53

Researcher Affiliations

Vannucci, Fabio A
  • Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St, Paul, MN, 55108, USA. vannu008@umn.edu.
Pusterla, Nicola
    Mapes, Samantha M
      Gebhart, Connie

        MeSH Terms

        • Animals
        • Bacterial Shedding
        • Desulfovibrionaceae Infections / immunology
        • Desulfovibrionaceae Infections / microbiology
        • Disease Susceptibility / immunology
        • Disease Susceptibility / microbiology
        • Disease Susceptibility / veterinary
        • Feces / microbiology
        • Horse Diseases / immunology
        • Horse Diseases / microbiology
        • Horses
        • Immunoenzyme Techniques / veterinary
        • Immunoglobulin G / blood
        • Intestinal Diseases / immunology
        • Intestinal Diseases / microbiology
        • Intestinal Diseases / veterinary
        • Lawsonia Bacteria / isolation & purification
        • Lawsonia Bacteria / physiology
        • Polymerase Chain Reaction / veterinary
        • Random Allocation
        • Species Specificity
        • Swine
        • Swine Diseases / immunology
        • Swine Diseases / microbiology

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        Citations

        This article has been cited 12 times.
        1. Suarez-Duarte ME, Santos RL, Pereira CER, Resende TP, Araujo MD, Correia PA, Barbosa JCR, Laub RP, Rodrigues DLN, Aburjaile FF, Guedes RMC. In Silico Evaluation of Lawsonia intracellularis Genes Orthologous to Genes Associated with Pathogenesis in Other Intracellular Bacteria. Microorganisms 2024 Aug 6;12(8).
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