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Home / Equine Research Bank / PLoS Negl Trop Dis / Evidence of Leptospiral Presence in the Cumberland Gap Regio...
PLoS neglected tropical diseases2019; 13(12); e0007990; doi: 10.1371/journal.pntd.0007990

Evidence of Leptospiral Presence in the Cumberland Gap Region.

Abstract: Leptospirosis is a widespread zoonotic disease that causes reproductive losses and/or hepatorenal failure in a number of animal species. Wild reservoirs of the disease, such as rodents, harbor the causative bacterium, Leptospira spp., in their kidneys and contaminate the environment by excreting infected urine. In this study, we tested small wild mammals, environmental water, and livestock in the Cumberland Gap region of southeastern Appalachia for the presence of pathogenic Leptospira or leptospiral antibodies. Small wild mammals (n = 101) and environmental water samples (n = 89) were screened by a real time quantitative PCR that targets the pathogenic Leptospira-specific lipl32 gene. Kidneys from 63 small wild mammals (62.37%) and two water sources (2.25%) tested positive for leptospiral DNA. To identify the infecting leptospiral species in qPCR-positive water and kidney samples, a fragment of leptospiral rpoB gene was PCR amplified and sequenced. L. kirschneri and L. interrogans were the leptospiral species carried by small wild mammals. Furthermore, sera from livestock (n = 52; cattle and horses) were screened for leptospiral antibodies using microscopic agglutination test (MAT). Twenty sera (38.46%) from livestock had antibodies to one or more serovars of pathogenic Leptospira spp. In conclusion, results from our study show exposure to leptospiral infection in farm animals and the presence of this zoonotic pathogen in the environmental water and kidneys of a significant number of small wild mammals. The public health implications of these findings remain to be assessed.
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Publication Date: 2019-12-26 PubMed ID: 31877135PubMed Central: PMC6952108DOI: 10.1371/journal.pntd.0007990Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article documents an investigation of the prevalence of Leptospira, a bacterium that causes leptospirosis, in the Cumberland Gap region. The study included tests on small wild mammals, environmental water, and livestock, revealing a significant presence of pathogenic Leptospira.

Research Goals and Methods

  • The research was designed with the aim to understand the prevalence of leptospirosis, a zoonotic disease that affects several animal species, causing reproductive losses and hepatorenal issues.
  • The researchers sampled small wild mammals and environmental water in the Cumberland Gap region of southeastern Appalachia. They also screened livestock to study the prevalence and spread of the leptospiral infection.
  • The samples were tested using real-time quantitative PCR, specifically targeting the pathogenic Leptospira-specific lipl32 gene, to identify the presence of leptospiral DNA.

Results and Findings

  • The research found that 62.37% of the sampled kidneys from small wild mammals and 2.25% of the water sources contained leptospiral DNA. This indicates a substantial presence of Leptospira bacterium in the wild animal population and environmental water in the area.
  • Additionally, by amplifying and sequencing a fragment of leptospiral rpoB gene in qPCR-positive water and kidney samples, researchers were able to identify the infecting leptospiral species, L. kirschneri and L. interrogans, carried by small wild mammals.
  • Analysis of sera from livestock (cattles and horses) using the microscopic agglutination test (MAT) revealed that 38.46% had antibodies to one or more serovars of pathogenic Leptospira spp.

Implications

  • The findings underline the exposure to leptospiral infection in farm animals and highlight the presence of the zoonotic pathogen in environmental water sources and kidneys of a large number of small wild mammals.
  • The presence of leptospirosis in livestock animals suggests a possible risk of transmission to humans, underlining the potential public health risk.
  • While the study sighs light on the extent of leptospiral spread within the studied region, it underlines that the public health implications of the findings need further detailed assessment.

Cite This Article

APA
Verma A, Beigel B, Smola CC, Kitts-Morgan S, Kish D, Nader P, Morgan J, Roberson J, Christmann U, Gruszynski K, Brandt L, Cho E, Murphy K, Goss R. (2019). Evidence of Leptospiral Presence in the Cumberland Gap Region. PLoS Negl Trop Dis, 13(12), e0007990. https://doi.org/10.1371/journal.pntd.0007990

Publication

PLoS neglected tropical diseases
ISSN: 1935-2735
NlmUniqueID: 101291488
Country: United States
Language: English
Volume: 13
Issue: 12
Pages: e0007990

Researcher Affiliations

Verma, Ashutosh
  • Center for Infectious, Zoonotic and Vector-borne diseases, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Beigel, Brittney
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Smola, Christopher Carl
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Kitts-Morgan, Susanna
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Kish, Daniel
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Nader, Paul
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Morgan, Joey
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Roberson, Jerry
  • Center for Infectious, Zoonotic and Vector-borne diseases, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Christmann, Undine
  • Center for Infectious, Zoonotic and Vector-borne diseases, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Gruszynski, Karen
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
  • Center for Animal and Human Health in Appalachia, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Brandt, LaRoy
  • Cumberland Mountain Research Center, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
  • School of Mathematics and Science, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Cho, Ellen
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Murphy, Kelly
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Goss, Ryan
  • College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.

MeSH Terms

  • Animals
  • Animals, Domestic
  • Appalachian Region / epidemiology
  • Bacterial Outer Membrane Proteins / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Kidney / microbiology
  • Leptospira / classification
  • Leptospira / genetics
  • Leptospira / isolation & purification
  • Leptospirosis / epidemiology
  • Leptospirosis / microbiology
  • Leptospirosis / veterinary
  • Lipoproteins / genetics
  • Real-Time Polymerase Chain Reaction
  • Rodentia
  • Water Microbiology

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 42 references
  1. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM. Leptospirosis: a zoonotic disease of global importance.. Lancet Infect Dis 3: 757–771.
    doi: 10.1016/s1473-3099(03)00830-2pubmed: 14652202google scholar: lookup
  2. Levett PN. Leptospirosis.. Clin Microbiol Rev 14: 296–326.
    doi: 10.1128/CMR.14.2.296-326.2001pmc: PMC88975pubmed: 11292640google scholar: lookup
  3. Ko AI, Goarant C, Picardeau M. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen.. Nat Rev Microbiol 7: 736–747.
    doi: 10.1038/nrmicro2208pmc: PMC3384523pubmed: 19756012google scholar: lookup
  4. Costa F, Hagan JE, Calcagno J, Kane M, Torgerson P, Martinez-Silveira MS. Global Morbidity and Mortality of Leptospirosis: A Systematic Review.. PLoS Negl Trop Dis 9(9): e0003898.
    doi: 10.1371/journal.pntd.0003898pmc: PMC4574773pubmed: 26379143google scholar: lookup
  5. Adler B, de la Pena Moctezuma A. Leptospira and leptospirosis.. Vet Microbiol 140: 287–296.
    doi: 10.1016/j.vetmic.2009.03.012pubmed: 19345023google scholar: lookup
  6. Faine S, Adler B, Bolin C, Perolat P. Leptospira and Leptospirosis, 2nd edition, MediSci.. .
  7. Carter CN, Cohen N, Steinman MN, Smith JL, Erol E, Brown S. Seroepidemiology of equine leptospirosis utilizing diagnostic laboratory specimens from 29 states (US) and one Canadian province.. Proceedings of 55th Annual AAVLD Meet p51.
  8. Ward MP, Glickman LT, Guptill LE. Prevalence of and risk factors for leptospirosis among dogs in the United States and Canada: 677 cases (1970–1998).. J Am Vet Med Assoc 220(1):53–58.
    doi: 10.2460/javma.2002.220.53pubmed: 12680448google scholar: lookup
  9. Glickman LT, Moore GE, Glickman NW, Caldanaro RJ, Aucoin D, Lewis HB. Purdue University-Banfield National Companion Animal Surveillance Program for emerging and zoonotic diseases.. Vector Borne Zoonotic Dis 6(1):14–23.
    doi: 10.1089/vbz.2006.6.14pubmed: 16584323google scholar: lookup
  10. Moore GE, Guptill LF, Glickman NW, Caldanaro RJ, Aucoin D, Glickman LT. Canine leptospirosis, United States, 2002–2004.. Emerg Infect Dis 12(3):501–503.
    doi: 10.3201/eid1203.050809pmc: PMC3291439pubmed: 16704794google scholar: lookup
  11. White AM, Zambrana-Torrelio C, Allen T, Rostal MK, Wright AK, Ball EC, Daszak P, Karesh WB. Hotspots of canine leptospirosis in the United States of America.. Vet J 222:29–35.
    doi: 10.1016/j.tvjl.2017.02.009pubmed: 28410673google scholar: lookup
  12. http://www.tnwatchablewildlife.org
  13. Vein J, Perrin A, Berny PJ, Benoit E, Leblond A, Kodjo A. Adaptation of a real-time PCR method for the detection and quantification of pathogenic leptospires in environmental water.. Can J Microbiol 58(7):828–835.
    doi: 10.1139/w2012-060pubmed: 22698616google scholar: lookup
  14. Gilpin BJ, Devane M, Nourozi F, Robson B, Scholes P, Lin S. Recommendations for the processing and storage of water samples before polymerase chain reaction (PCR) analysis. New Zealand Journal of Marine and Freshwater Research 47:4, 582–586.
  15. Rawlins J, Portanova A, Zuckerman I, Loftis A, Ceccato P, Willingham AL, Verma A. Molecular detection of leptospiral DNA in environmental water on St. Kitts.. Int J Environ Res Public Health 11(8):7953–7960.
    doi: 10.3390/ijerph110807953pmc: PMC4143842pubmed: 25105546google scholar: lookup
  16. Levett PN, Morey RE, Galloway RL, Turner DE, Steigerwalt AG, Mayer LW. Detection of pathogenic leptospires by real-time quantitative PCR.. J Med Microbiol 54 (Pt 1):45–49.
    doi: 10.1099/jmm.0.45860-0pubmed: 15591254google scholar: lookup
  17. Stoddard RA, Gee JE, Wilkins PP, McCaustland K, Hoffmaster AR. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene.. Diagn Microbiol Infect Dis 64(3):247–255.
    doi: 10.1016/j.diagmicrobio.2009.03.014pubmed: 19395218google scholar: lookup
  18. La Scola B, Bui LT, Baranton G, Khamis A, Raoult D. Partial rpoB gene sequencing for identification of Leptospira species.. FEMS Microbiol Lett 263(2):142–147.
    doi: 10.1111/j.1574-6968.2006.00377.xpubmed: 16978348google scholar: lookup
  19. Schneider AG, Casanovas-Massana A, Hacker KP, Wunder EA Jr, Begon M, Reis MG, Childs JE, Costa F, Lindow JC, Ko AI. Quantification of pathogenic Leptospira in the soils of a Brazilian urban slum.. PLoS Negl Trop Dis 12(4):e0006415.
    doi: 10.1371/journal.pntd.0006415pmc: PMC5906024pubmed: 29624576google scholar: lookup
  20. World Health Organization. Human leptospirosis: Guidance for diagnosis, surveillance and control. .
  21. Briskin EA, Casanovas-Massana A, Ryff KR, Morales-Estrada S, Hamond C, Perez-Rodriguez NM, Benavidez KM, Weinberger DM, Castro-Arellano I, Wunder EA, Sharp TM, Rivera-Garcia B, Ko AI. Seroprevalence, risk factors, and rodent reservoirs of leptospirosis in an urban community of Puerto Rico, 2015.. J Infect Dis pii: jiz339.
    doi: 10.1093/infdis/jiz339pmc: PMC6761939pubmed: 31342075google scholar: lookup
  22. Vieira AS, Narduche L, Martins G, Schabib Péres IA, Zimmermann NP, Juliano RS, Pellegrin AO, Lilenbaum W. Detection of wild animals as carriers of Leptospira by PCR in the Pantanal biome, Brazil.. Acta Trop 163:87–89.
    doi: 10.1016/j.actatropica.2016.08.001pubmed: 27496621google scholar: lookup
  23. Shiokawa K, Llanes A, Hindoyan A, Cruz-Martinez L, Welcome S, Rajeev S. Peridomestic small Indian mongoose: An invasive species posing as potential zoonotic risk for leptospirosis in the Caribbean.. Acta Trop 190:166–170.
    doi: 10.1016/j.actatropica.2018.11.019pubmed: 30465742google scholar: lookup
  24. Himsworth CG, Bidulka J, Parsons KL, Feng AY, Tang P, Jardine CM, Kerr T, Mak S, Robinson J, Patrick DM. Ecology of Leptospira interrogans in Norway rats (Rattus norvegicus) in an inner-city neighborhood of Vancouver, Canada.. PLoS Negl Trop Dis 7(6):e2270.
    doi: 10.1371/journal.pntd.0002270pmc: PMC3688548pubmed: 23818996google scholar: lookup
  25. Runge M, von Keyserlingk M, Braune S, Becker D, Plenge-Bönig A, Freise JF, Pelz HJ, Esther A. Distribution of rodenticide resistance and zoonotic pathogens in Norway rats in Lower Saxony and Hamburg, Germany.. Pest Manag Sci 69(3):403–408.
    doi: 10.1002/ps.3369pubmed: 22888034google scholar: lookup
  26. Aviat F, Blanchard B, Michel V, Blanchet B, Branger C, Hars J, Mansotte F, Brasme L, De Champs C, Bolut P, Mondot P, Faliu J, Rochereau S, Kodjo A, Andre-Fontaine G. Leptospira exposure in the human environment in France: A survey in feral rodents and in fresh water.. Comp Immunol Microbiol Infect Dis 32(6):463–476.
    doi: 10.1016/j.cimid.2008.05.004pubmed: 18639932google scholar: lookup
  27. Trueba G, Zapata S, Madrid K, Cullen P, Haake D. Cell aggregation: a mechanism of pathogenic Leptospira to survive in fresh water.. Int Microbiol 7(1):35–40.
    pubmed: 15179605
  28. Smith DJ, Self HR. Observations on the survival of Leptospira australis A in soil and water.. J Hyg (Lond) 53(4):436–444.
    pmc: PMC2217981pubmed: 13278528
  29. Yupiana Y, Wilson PR, Weston JF, Vallée E, Collins-Emerson JM, Benschop J, Scotland T, Heuer C. Epidemiological investigation of Leptospira spp. in a dairy farming enterprise after the occurrence of three human leptospirosis cases.. Zoonoses Public Health 66(5):470–479.
    doi: 10.1111/zph.12578pubmed: 30942554google scholar: lookup
  30. Siqueira CC, Fraga DBM, Chagas-Junior AD, Athanazio DA, Silva MMN, Cerqueira RB, da C McBride FW, Pinna MH, Ayres MCC. Seroprevalence and risk factors associated with equine leptospirosis in the metropolitan region of Salvador and Recôncavo Baiano region, Bahia state (NE Brazil).. Trop Anim Health Prod .
    doi: 10.1007/s11250-019-01956-5pubmed: 31289965google scholar: lookup
  31. Divers TJ, Chang YF, Irby NL, Smith JL, Carter CN. Leptospirosis: An important infectious disease in North American horses.. Equine Vet J 51(3):287–292.
    doi: 10.1111/evj.13069pubmed: 30629756google scholar: lookup
  32. Mayer-Scholl A, Hammerl JA, Schmidt S, Ulrich RG, Pfeffer M, Woll D, Scholz HC, Thomas A, Nöckler K. Leptospira spp. in rodents and shrews in Germany.. Int J Environ Res Public Health 11(8):7562–7574.
    doi: 10.3390/ijerph110807562pmc: PMC4143818pubmed: 25062275google scholar: lookup
  33. Turk N, Milas Z, Margaletic J, Staresina V, Slavica A, Riquelme-Sertour N, Bellenger E, Baranton G, Postic D. Molecular characterization of Leptospira spp. strains isolated from small rodents in Croatia.. Epidemiol Infect 130(1):159–166.
    doi: 10.1017/s0950268802008026pmc: PMC2869950pubmed: 12613757google scholar: lookup
  34. Kositanont U, Naigowit P, Imvithaya A, Singchai C, Puthavathana P. Prevalence of antibodies to Leptospira serovars in rodents and shrews trapped in low and high endemic areas in Thailand.. J Med Assoc Thai 86(2):136–142.
    pubmed: 12678151
  35. Krijger IM, Ahmed AAA, Goris MGA, Groot Koerkamp PWG, Meerburg BG. Prevalence of Leptospira Infection in Rodents from Bangladesh.. Int J Environ Res Public Health 16(12).
    pmc: PMC6616592pubmed: 31207905
  36. Ciceroni L, Stepan E, Pinto A, Pizzocaro P, Dettori G, Franzin L, Lupidi R, Mansueto S, Manera A, Ioli A, Marcuccio L, Grillo R, Ciarrocchi S, Cinco M. Epidemiological trend of human leptospirosis in Italy between 1994 and 1996.. Eur J Epidemiol 16(1):79–86.
    doi: 10.1023/a:1007658607963pubmed: 10780347google scholar: lookup
  37. Jansen A, Schöneberg I, Frank C, Alpers K, Schneider T, Stark K. Leptospirosis in Germany, 1962–2003.. Emerg Infect Dis 11(7):1048–1054.
    doi: 10.3201/eid1107.041172pmc: PMC3371786pubmed: 16022779google scholar: lookup
  38. Hochedez P, Escher M, Decoussy H, Pasgrimaud L, Martinez R, Rosine J, Théodose R, Bourhy P, Picardeau M, Olive C, Ledrans M, Cabie A. Outbreak of leptospirosis among canyoning participants, Martinique, 2011.. Euro Surveill 18(18):20472.
    pubmed: 23725775
  39. Schmalzle SA, Tabatabai A, Mazzeffi M, Matta A, Hollis A, Zubrow M, Rajagopal K, Thom K, Scalea T. Recreational 'mud fever': Leptospira interrogans induced diffuse alveolar hemorrhage and severe acute respiratory distress syndrome in a U.S. Navy seaman following 'mud-run' in Hawaii.. IDCases 15:e00529.
    doi: 10.1016/j.idcr.2019.e00529pmc: PMC6441746pubmed: 30976519google scholar: lookup
  40. Muñoz-Zanzi C, Mason MR, Encina C, Astroza A, Romero A. Leptospira contamination in household and environmental water in rural communities in southern Chile.. Int J Environ Res Public Health 11(7):6666–6680.
    doi: 10.3390/ijerph110706666pmc: PMC4113836pubmed: 24972030google scholar: lookup
  41. Ganoza CA, Matthias MA, Collins-Richards D, Brouwer KC, Cunningham CB, Segura ER, Gilman RH, Gotuzzo E, Vinetz JM. Determining risk for severe leptospirosis by molecular analysis of environmental surface waters for pathogenic Leptospira.. PLoS Med 3(8):e308.
    doi: 10.1371/journal.pmed.0030308pmc: PMC1551915pubmed: 16933963google scholar: lookup
  42. Casanovas-Massana A, Costa F, Riediger IN, Cunha M, de Oliveira D, Mota DC, Sousa E, Querino VA, Nery N Jr, Reis MG, Wunder EA Jr, Diggle PJ, Ko AI. Spatial and temporal dynamics of pathogenic Leptospira in surface waters from the urban slum environment.. Water Res 130:176–184.
    doi: 10.1016/j.watres.2017.11.068pmc: PMC5767135pubmed: 29220718google scholar: lookup
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