Evolution of 17-β-estradiol, estrone and estrone-sulfate concentrations in late pregnancy of different breeds of mares using Liquid Chromatography and Mass Spectrometry.
Abstract: This study describes 17-β-estradiol (E2), estrone (E1) and estrone-sulfate (E1S) concentrations between 4 and 11 months in healthy equine pregnancies of two different breeds using Liquid Chromatography coupled to Mass-Spectrometry (LC-MS). In 2 stud-farms including 15 Spanish PureBred (SPB) and 11 Showjumping (SJ) types mares, combined thickness of the uterus and the placenta (CTUP) was measured and blood was sampled monthly between 4 and 11 months of gestation. Concentrations of E2, E1 and E1S were assayed with LC-MS in mares with normal CTUP. Effects of breed, day of pregnancy and mare's parity and age on estrogens concentrations were investigated. Peak of E2 was observed at 5 months (median: 46.4 pg/mL; maximum: 201.5 pg/mL). A strong correlation was observed between E1 and E1S (p < 0.0001, r = 0.85). Peak of E1 (median: 571.0 pg/mL; maximum: 1641.9 pg/mL) and E1S (median: 573.6 ng/mL; maximum: 997.6 ng/mL) concentrations was observed at the 5th month and then E1S decreased quicker than E1 until the end of pregnancy. Higher E2 and E1 concentrations were observed in SJ than in SPB mares between the 6th and the 8th months. No difference between breeds was observed for E1S monthly evolution. Estrogen peak values were all observed at 5 months. Unlike recent LC-MS studies, E1S values observed here were in the same range than those previously established using immuno-assays. After the 6th month, E1S decreased quicker than E1. Effect of breed only observed on non-sulfonated estrogens should be further confirmed. These findings confirm that sulfonation activity of the allantochorion may be limited after the 6th month.
Copyright © 2022 Elsevier Inc. All rights reserved.
Publication Date: 2022-06-11 PubMed ID: 35738034DOI: 10.1016/j.theriogenology.2022.06.004Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the concentration of three types of estrogens in two different horse breeds during late pregnancy and observes how factors such as breed, stage of pregnancy, mare’s age and parity affect these levels.
Research Methodology
- The study was conducted in two stud-farms including 15 Spanish PureBred (SPB) and 11 Showjumping (SJ) type horses.
- Between the 4th and 11th months of pregnancy, monthly blood samples were taken from the healthy mares, and the combined thickness of the uterus and the placenta was measured.
- The blood samples were then assayed to measure the concentration of three estrogens – 17-β-estradiol (E2), estrone (E1) and estrone-sulfate (E1S) using Liquid Chromatography coupled to Mass-Spectrometry (LC-MS).
Research Findings
- The peak of E2 was observed at the 5th month of pregnancy, with a median concentration of 46.4 pg/mL and a maximum concentration of 201.5 pg/mL.
- A strong correlation was observed between the concentration of E1 and E1S (p < 0.0001, r = 0.85).
- The peaks of E1 and E1S concentrations were also observed at the 5th month of pregnancy, but after this, E1S decreased more quickly than E1 until the end of the pregnancy.
- Higher E2 and E1 concentrations were observed in SJ mares as opposed to SPB mares between the 6th and the 8th months of pregnancy.
- No difference was observed in the monthly evolution of E1S between the two breeds.
Conclusion and Further Study
- The findings of this study confirm that the sulfonation activity of the allantochorion may be limited after the 6th month of pregnancy.
- The effect of breed on non-sulfonated estrogens needs further confirmation as it was only observed in this study and has not been corroborated by other research.
- The E1S values observed in this study were in the same range as those established using immuno-assay techniques, contradicting recent LC-MS studies which have reported different results.
Cite This Article
APA
Ledeck J, Dufour P, Evrard É, Le Goff C, Peeters S, Brutinel F, Egyptien S, Deleuze S, Cavalier É, Ponthier J.
(2022).
Evolution of 17-β-estradiol, estrone and estrone-sulfate concentrations in late pregnancy of different breeds of mares using Liquid Chromatography and Mass Spectrometry.
Theriogenology, 189, 86-91.
https://doi.org/10.1016/j.theriogenology.2022.06.004 Publication
Researcher Affiliations
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium.
- Clinical Chemistry, University Hospital (CHU), Liège University, Belgium.
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium.
- Clinical Chemistry, University Hospital (CHU), Liège University, Belgium.
- Clinical Chemistry, University Hospital (CHU), Liège University, Belgium.
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium.
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium.
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium.
- Clinical Chemistry, University Hospital (CHU), Liège University, Belgium.
- Equine Theriogenology, Equine Clinical Sciences Department, FARAH Comparative Veterinary Medicine, Liège University, Belgium. Electronic address: Jerome.Ponthier@uliege.be.
MeSH Terms
- Animals
- Chromatography, Liquid / veterinary
- Estradiol
- Estrogens
- Estrone
- Female
- Horses
- Mass Spectrometry / veterinary
- Pregnancy
- Sulfates
Conflict of Interest Statement
Declaration of competing interest Authors have no conflict of interest to declare.
Citations
This article has been cited 1 times.- Miller JL, Reddy A, Harman RM, Van de Walle GR. A xenotransplantation mouse model to study physiology of the mammary gland from large mammals. PLoS One 2024;19(2):e0298390.
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